BBa_K2053000 1 BBa_K2053000 T. pseudonana silaffin kinase (tpSTK1) 2016-10-10T11:00:00Z 2016-10-11T05:16:42Z This part comes from Thalassiosira pseudonana and we took the sequence by using its GenBank accession number (EED92887) [Sheppard V et al, J Biol Chem. 2010 Jan 8;285(2):1166-76, doi: 10.1074/jbc.M109.039529, Epub 2009 Nov 4]. Thalassiosira pseudonana silaffin kinase (tpSTK1) phosphorylates diatom silaffins. We propose to use it for this property. Indeed, we hope that phosphorylation of silica-binding peptide increases its biosilification properties. false false _2521_ 31618 31618 9 false Polyhistidine tag (6 His) was added at the N-terminal false Mathieu Hubert annotation2492897 1 His-Tag range2492897 1 4 21 annotation2492901 1 TEV cleavage site range2492901 1 22 42 annotation2493026 1 tpSTK1 range2493026 1 43 1569 annotation2492900 1 Stop codon range2492900 1 1570 1572 annotation2492898 1 Start codon range2492898 1 1 3 annotation2492899 1 Stop codon range2492899 1 1573 1575 BBa_K2053000_sequence 1 atgcatcaccatcaccatcacgaaaacctgtattttcagggccgtgttatccgtaacgtttctctggttgttatcgttacctcttgcaccttcctggtttctgttacctctttcacctctcagcgtaccgttacctctgcgtctccgtcttacaccaactctaacaacaacctgtactctccgccgctgttcgcgtctggtccgccggttatctctaactggcgttaccagtcttcttctggtaccatctctggtaccgttaccaaccacccgaccatcccggacggtgactctatcaccacctctccggttcgtgacggtaacggtatcaacgaaaacaccatcgttcagaccaaatctggttctaaatacaaactgtctaacgttgcgtggggtgcgaccatcccggcggttgaaaccaaaaaagcgatgcagaaagaagcggaagcggcggaaaaagcgcgtaaagttgaagcggaaaaagcgaaaaccgcggcggcgaaagcgaaaaccgcgtctaaagcgaccccgaaagcgacctctccggcggcggcgaccaccaaacagccgtcttctgcggaactgcgtcgtctggcgaaatctaccttctctctgaccggcaacaccgttggttctaacggtaaatacctgctggcgggtaaaccgcgtcagtcttctggtcgtgcggcgaaaatctggaccgcgtacatcgcggacccgatcgaaccgggtgttccgctgggtttctctgaaggtgacgaatctaaagttcagccgctgaccatcaaactgtctccggacgttgaccgtctgtacctggaaaacgcgaactacaacaaagttcagtctggtctgttcatgggtcgtttcgttaaaaaaatggactacatcgaaaacgttccgtcttctgaccgttctctggacggtaaagtttctgcgctgatcatcgaatctggtcagtacgacctgaaagcgctgctgtctgcgcgtaaaggtctgccgctggcgggtaaagcgctgcgtgacgcggcggcggcggcgggtcagtgcatccaggcggttcactcttctaacctggtttggaccgacctgaaaaccgaaaacttcatcgttgttcgtaacggtaacctgtaccaggaagttgaagacgttaaaggttctatgggtctgccgggtgttaaaggtatcgacctggaatctgttatcgcgaaaggtggtaacccgatcgactactctccggaagcgtgcccgccggaatttgcgaaagcgtacatggaaggtatcggtggtgacttcgttctggactactcttacgacatgtggtctctgggtatgatgctgtacgaactgtctaccggcaaagcgtacttcgacaaaaaaggtccgtctcaggttaccaaactgctgtgcaccgacaccttcgaagcggacgtttctgcggttccggacgaaaaactgcgtgacctgatcggtaactgcctgaacctggacccgcgtaaacgtccggacatcaccggcttcctgctgcacccgtacttcctgctgtctggtatcggtccgatctctttctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z