BBa_K2053000
1
BBa_K2053000
T. pseudonana silaffin kinase (tpSTK1)
2016-10-10T11:00:00Z
2016-10-11T05:16:42Z
This part comes from Thalassiosira pseudonana and we took the sequence by using its GenBank accession number (EED92887) [Sheppard V et al, J Biol Chem. 2010 Jan 8;285(2):1166-76, doi: 10.1074/jbc.M109.039529, Epub 2009 Nov 4].
Thalassiosira pseudonana silaffin kinase (tpSTK1) phosphorylates diatom silaffins. We propose to use it for this property. Indeed, we hope that phosphorylation of silica-binding peptide increases its biosilification properties.
false
false
_2521_
31618
31618
9
false
Polyhistidine tag (6 His) was added at the N-terminal
false
Mathieu Hubert
annotation2492901
1
TEV cleavage site
range2492901
1
22
42
annotation2492897
1
His-Tag
range2492897
1
4
21
annotation2492899
1
Stop codon
range2492899
1
1573
1575
annotation2493026
1
tpSTK1
range2493026
1
43
1569
annotation2492900
1
Stop codon
range2492900
1
1570
1572
annotation2492898
1
Start codon
range2492898
1
1
3
BBa_K2053000_sequence
1
atgcatcaccatcaccatcacgaaaacctgtattttcagggccgtgttatccgtaacgtttctctggttgttatcgttacctcttgcaccttcctggtttctgttacctctttcacctctcagcgtaccgttacctctgcgtctccgtcttacaccaactctaacaacaacctgtactctccgccgctgttcgcgtctggtccgccggttatctctaactggcgttaccagtcttcttctggtaccatctctggtaccgttaccaaccacccgaccatcccggacggtgactctatcaccacctctccggttcgtgacggtaacggtatcaacgaaaacaccatcgttcagaccaaatctggttctaaatacaaactgtctaacgttgcgtggggtgcgaccatcccggcggttgaaaccaaaaaagcgatgcagaaagaagcggaagcggcggaaaaagcgcgtaaagttgaagcggaaaaagcgaaaaccgcggcggcgaaagcgaaaaccgcgtctaaagcgaccccgaaagcgacctctccggcggcggcgaccaccaaacagccgtcttctgcggaactgcgtcgtctggcgaaatctaccttctctctgaccggcaacaccgttggttctaacggtaaatacctgctggcgggtaaaccgcgtcagtcttctggtcgtgcggcgaaaatctggaccgcgtacatcgcggacccgatcgaaccgggtgttccgctgggtttctctgaaggtgacgaatctaaagttcagccgctgaccatcaaactgtctccggacgttgaccgtctgtacctggaaaacgcgaactacaacaaagttcagtctggtctgttcatgggtcgtttcgttaaaaaaatggactacatcgaaaacgttccgtcttctgaccgttctctggacggtaaagtttctgcgctgatcatcgaatctggtcagtacgacctgaaagcgctgctgtctgcgcgtaaaggtctgccgctggcgggtaaagcgctgcgtgacgcggcggcggcggcgggtcagtgcatccaggcggttcactcttctaacctggtttggaccgacctgaaaaccgaaaacttcatcgttgttcgtaacggtaacctgtaccaggaagttgaagacgttaaaggttctatgggtctgccgggtgttaaaggtatcgacctggaatctgttatcgcgaaaggtggtaacccgatcgactactctccggaagcgtgcccgccggaatttgcgaaagcgtacatggaaggtatcggtggtgacttcgttctggactactcttacgacatgtggtctctgggtatgatgctgtacgaactgtctaccggcaaagcgtacttcgacaaaaaaggtccgtctcaggttaccaaactgctgtgcaccgacaccttcgaagcggacgtttctgcggttccggacgaaaaactgcgtgacctgatcggtaactgcctgaacctggacccgcgtaaacgtccggacatcaccggcttcctgctgcacccgtacttcctgctgtctggtatcggtccgatctctttctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z