LOCUS       BBa_K2053002             777 bp    DNA     linear       23-Dec-2024
DEFINITION  Fusion Si4-cellulose-binding domain-B domain protein A
ACCESSION   BBa_K2053002
VERSION     BBa_K2053002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..36
                     /label=pET43.1a
     RBS             23..28
                     /label=RBS
     misc_feature    37..39
                     /label=Start codon
     misc_feature    40..57
                     /label=His-Tag
     misc_feature    58..78
                     /label=TEV cleavage site
     CDS             79..123
                     /label=Si4
     CDS             124..159
                     /label=Linker 1
     CDS             160..465
                     /label=CBPa
     CDS             466..501
                     /label=Linker 2
     CDS             502..723
                     /label=BpA
     misc_feature    724..726
                     /label=Stop codon
     misc_feature    727..729
                     /label=Stop codon
     misc_feature    730..776
                     /label=Terminator T7
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgc atcaccatca ccatcacgaa
       61 aacctgtatt ttcagggcgg cggtgggact catcaccatc gccctcatcc acatccgtcg
      121 atgggcagcg cgggcagcgc ggcgggcagc ggcgaatttg ccggctcatc aatgtcagtt
      181 gaattttaca actctaacaa atcagcacaa acaaactcaa ttacaccaat aatcaaaatt
      241 actaacacat ctgacagtga tttaaattta aatgacgtaa aagttagata ttattacaca
      301 agtgatggta cacaaggaca aactttctgg tgtgaccatg ctggtgcatt attaggaaat
      361 agctatgttg ataacactag caaagtgaca gcaaacttcg ttaaagaaac agcaagccca
      421 acatcaacct atgatacata tgttgaattt ggatttgcaa ccggtggcgg tgcgggcagc
      481 ggtagcggtg gcagcggcgc gtctaaaacc gcggcgctgg cgcagcacga cgaagcggtt
      541 gacaacaaat tcaacaaaga acagcagaac gcgttctacg aaatcctgca cctgccgaac
      601 ctgaacgaag aacagcgtaa cgcgttcatc cagtctctga aagacgaccc gtctcagtct
      661 gcgaacctgc tggcggaagc gaaaaaactg aacgacgcgc aggcgccgaa agttgacggt
      721 tcttaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttg
//