##gff-version 3
##sequence-region BBa_K2053002 1 776
BBa_K2053002	.	null	1	36	.	+	0	ID=annotation2493012;Name=pET43.1a
BBa_K2053002	.	CDS	466	501	.	+	0	ID=annotation2493020;Name=Linker 2
BBa_K2053002	.	sequence_feature	730	776	.	+	0	ID=annotation2493024;Name=Terminator T7
BBa_K2053002	.	CDS	160	465	.	+	0	ID=annotation2493019;Name=CBPa
BBa_K2053002	.	tag	40	57	.	+	0	ID=annotation2493015;Name=His-Tag
BBa_K2053002	.	stop_codon	727	729	.	+	0	ID=annotation2493023;Name=Stop codon
BBa_K2053002	.	sequence_feature	58	78	.	+	0	ID=annotation2493016;Name=TEV cleavage site
BBa_K2053002	.	stop_codon	724	726	.	+	0	ID=annotation2493022;Name=Stop codon
BBa_K2053002	.	CDS	79	123	.	+	0	ID=annotation2493017;Name=Si4
BBa_K2053002	.	ribosome_entry_site	23	28	.	+	0	ID=annotation2493013;Name=RBS
BBa_K2053002	.	CDS	124	159	.	+	0	ID=annotation2493018;Name=Linker 1
BBa_K2053002	.	start_codon	37	39	.	+	0	ID=annotation2493014;Name=Start codon
BBa_K2053002	.	CDS	502	723	.	+	0	ID=annotation2493021;Name=BpA
>BBa_K2053002
aataattttgtttaactttaagaaggagatatacatatgcatcaccatcaccatcacgaaaacctgtattttcagggcg
gcggtgggactcatcaccatcgccctcatccacatccgtcgatgggcagcgcgggcagcgcggcgggcagcggcgaatt
tgccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaa
attactaacacatctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaag
gacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaa
cttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaaccggtggcggtgcg
ggcagcggtagcggtggcagcggcgcgtctaaaaccgcggcgctggcgcagcacgacgaagcggttgacaacaaattca
acaaagaacagcagaacgcgttctacgaaatcctgcacctgccgaacctgaacgaagaacagcgtaacgcgttcatcca
gtctctgaaagacgacccgtctcagtctgcgaacctgctggcggaagcgaaaaaactgaacgacgcgcaggcgccgaaa
gttgacggttcttaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg