BBa_K2053002
1
Si4-CBPa-B
Fusion Si4-cellulose-binding domain-B domain protein A
2016-10-10T11:00:00Z
2016-10-11T05:19:06Z
Silica-binding peptide (Si4) was taken from iGEM Leeds 2013 (BBa_K1028000), cellulose-binding domain of cellulose-binding protein A was taken from iGEM Bielefeld-Germany 2012 team (BBa_K863110) and B domain of protein A was taken from iGEM Warsaw 2008 (BBa_K103003).
It is a fusion protein between the phage displayed silica-binding peptide (Si4) developed by Naik et al (Journal of Nanoscience and Nanotechnology, Volume 2, Number 1, February 2002, pp. 95-100(6)), the Clostridium cellulovorans cellulose-binding domain of cellulose-binding protein A (CBPa) with the B domain of staphylococcus aureus protein A (BpA). This fusion protein is used to bind silica and antibodies to cellulose. We use these functions to functionalize a silica-cellulose composite-based patch into an immunodetection-based system.
false
false
_2521_
31618
31618
9
false
Polyhistidine tag (6 His) was added at the N-terminal end as well as linkers to join Si4 to CBPa and also CBPa to BpA. pET43.1a-derived ribosome-binding site (RBS) and T7 terminator were added.
false
Mathieu Hubert
annotation2493017
1
Si4
range2493017
1
79
123
annotation2493021
1
BpA
range2493021
1
502
723
annotation2493012
1
pET43.1a
range2493012
1
1
36
annotation2493013
1
RBS
range2493013
1
23
28
annotation2493014
1
Start codon
range2493014
1
37
39
annotation2493024
1
Terminator T7
range2493024
1
730
776
annotation2493015
1
His-Tag
range2493015
1
40
57
annotation2493016
1
TEV cleavage site
range2493016
1
58
78
annotation2493019
1
CBPa
range2493019
1
160
465
annotation2493022
1
Stop codon
range2493022
1
724
726
annotation2493020
1
Linker 2
range2493020
1
466
501
annotation2493018
1
Linker 1
range2493018
1
124
159
annotation2493023
1
Stop codon
range2493023
1
727
729
BBa_K2053002_sequence
1
aataattttgtttaactttaagaaggagatatacatatgcatcaccatcaccatcacgaaaacctgtattttcagggcggcggtgggactcatcaccatcgccctcatccacatccgtcgatgggcagcgcgggcagcgcggcgggcagcggcgaatttgccggctcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacatctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaaccggtggcggtgcgggcagcggtagcggtggcagcggcgcgtctaaaaccgcggcgctggcgcagcacgacgaagcggttgacaacaaattcaacaaagaacagcagaacgcgttctacgaaatcctgcacctgccgaacctgaacgaagaacagcgtaacgcgttcatccagtctctgaaagacgacccgtctcagtctgcgaacctgctggcggaagcgaaaaaactgaacgacgcgcaggcgccgaaagttgacggttcttaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z