BBa_K516030
1
BBa_K516030
mRFP protein generator TERM+ RBS+(B0030)
2011-09-07T11:00:00Z
2015-05-08T01:12:32Z
Registry
Released HQ 2013
mRFP protein generator TERM+ RBS+(B0030)
false
false
_681_
0
4150
9
In stock
true
--
false
Nicolo' Politi, Federica Sampietro, Davide Bianchini
component2250572
1
BBa_B0030
component2250576
1
BBa_E1010
component2250583
1
BBa_B0015
annotation2250583
1
BBa_B0015
range2250583
1
736
864
annotation2250572
1
BBa_B0030
range2250572
1
1
15
annotation2250576
1
BBa_E1010
range2250576
1
22
727
BBa_K2055000
1
BBa_K2055000
Gold/copper inducible regulator
2016-09-28T11:00:00Z
2016-10-19T08:24:26Z
Its organism of origin is Salmonella typhimurium.
DNA construct constituted by the pgolS promoter and the coding sequence of CueR. As the pgolS promoter has a basal transcription level, some CueR would be constantly expressed. CueR binds either copper or gold, though it is more selective for copper. CueR is a transcription factor that stimulates the transcription at the pgolS promoter when bound to copper/gold. Thus, this construct increases its expression in the presence of gold or copper through the production of a copper/gold sensitive protein.
false
false
_2523_
30725
30723
9
false
The coding sequence is optimized for its expression in C. violaceum
false
Yili Natalia Padilla Flores, Jos?? Garc??a Lima, Marcelo ??lvarez Mata
component2523933
1
BBa_B0034
component2523931
1
BBa_K2055069
component2523930
1
BBa_B0034
component2523947
1
BBa_K516030
component2523928
1
BBa_K2055169
annotation2523933
1
BBa_B0034
range2523933
1
491
502
annotation2523947
1
BBa_K516030
range2523947
1
511
1374
annotation2523928
1
BBa_K2055169
range2523928
1
1
39
annotation2523931
1
BBa_K2055069
range2523931
1
66
482
annotation2523930
1
BBa_B0034
range2523930
1
48
59
BBa_K2055069
1
CueR
cueR DNA-binding transcriptional regulator
2016-09-28T11:00:00Z
2016-09-29T04:34:44Z
Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
CueR binds either copper or gold, though it is more selective for copper. CueR is a transcription factor that stimulates the transcription at the pgolS promoter when bound to copper/gold.
false
false
_2523_
30723
30723
9
false
The coding sequence is optimized for its expression in C. violaceum.
false
Yili Natalia Padilla Flores
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K2055169
1
BBa_K2055169
Gold inducible promoter
2016-09-28T11:00:00Z
2016-09-29T05:35:57Z
Salmonella typhimurium LT2
It is positively regulated by the transcription factor GolS. GolS induces transcription from this promoter once GolS is activated upon binding a gold ion.
false
false
_2523_
30723
30723
9
false
This part was de novo synthesized by iGEM10_UIUC-Illinois.
http://parts.igem.org/Part:BBa_K1127008
false
Yili Natalia Padilla Flores
annotation2485449
1
pGolS
range2485449
1
1
39
BBa_E1010
1
mRFP1
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
2004-07-27T11:00:00Z
2015-08-31T04:07:26Z
Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a>
Released HQ 2013
monomeric RFP:
Red Fluorescent Protein.
Excitation peak: 584 nm
Emission peak: 607 nm
false
false
_11_1_
0
52
7
In stock
false
TAATAA double stop codon added (DE).
Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
true
Drew Endy
annotation1014044
1
mrfp1
range1014044
1
1
675
annotation2214014
1
Help:Barcodes
range2214014
1
682
706
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_E1010_sequence
1
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K516030_sequence
1
attaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K2055000_sequence
1
cttgaccttcccacaatggcaagctttaggctttctgattactagagaaagaggagaaatactagatgaatattagcgatgtggcgaaaaaaaccggtttaaccagcaaagccattcggttttatgaagagaaagggctggtgacgccgccattacgtagcgaaaatggttatcgcacctacacgcagaagcatttaaacgagctgacgttgctgcgacaggcgaggcaggtggggtttaatctggaagagtgtggcgaactggtcaatctgtttaacgatccgcgacgccatagcgcagatgtgaaaaagcggacgctggaaaaggtcgcggagatcgagcgccatatcagcgagctgcaatcaatgcgcgatcaactgttggcgctggcggagagttgtcccggcgatgacagcgccgactgcccgattattgataatctttccggctgctgtcatcacaaggcgcaaaagccacgttgatactagagaaagaggagaaatactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K2055069_sequence
1
atgaatattagcgatgtggcgaaaaaaaccggtttaaccagcaaagccattcggttttatgaagagaaagggctggtgacgccgccattacgtagcgaaaatggttatcgcacctacacgcagaagcatttaaacgagctgacgttgctgcgacaggcgaggcaggtggggtttaatctggaagagtgtggcgaactggtcaatctgtttaacgatccgcgacgccatagcgcagatgtgaaaaagcggacgctggaaaaggtcgcggagatcgagcgccatatcagcgagctgcaatcaatgcgcgatcaactgttggcgctggcggagagttgtcccggcgatgacagcgccgactgcccgattattgataatctttccggctgctgtcatcacaaggcgcaaaagccacgttga
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K2055169_sequence
1
cttgaccttcccacaatggcaagctttaggctttctgat
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z