##gff-version 3
##sequence-region BBa_K2055000 1 1374
BBa_K2055000	.	engineered_region	511	1374	.	+	0	ID=BBa_K516030;Name=BBa_K516030
BBa_K2055000	.	terminator	1246	1374	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516030
BBa_K2055000	.	terminator	1246	1325	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2055000	.	stem_loop	1257	1300	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2055000	.	engineered_region	1246	1325	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2055000	.	terminator	1334	1374	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2055000	.	engineered_region	1334	1374	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2055000	.	stop_codon	1367	1367	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2055000	.	stem_loop	1341	1360	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2055000	.	polyA_site	1361	1374	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2055000	.	ribosome_entry_site	511	525	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K516030
BBa_K2055000	.	engineered_region	511	525	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K2055000	.	ribosome_entry_site	518	522	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K2055000	.	ribosome_entry_site	511	525	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K2055000	.	CDS	532	1237	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K516030
BBa_K2055000	.	CDS	532	1206	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K2055000	.	engineered_tag	1213	1237	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K2055000	.	promoter	1	39	.	+	0	ID=BBa_K2055169;Name=BBa_K2055169
BBa_K2055000	.	promoter	1	39	.	+	0	ID=annotation2485449;Name=pGolS;Parent=BBa_K2055169
BBa_K2055000	.	ribosome_entry_site	491	502	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2055000	.	conserved_region	495	498	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2055000	.	ribosome_entry_site	48	59	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2055000	.	conserved_region	52	55	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2055000	.	CDS	66	482	.	+	0	ID=BBa_K2055069;Name=BBa_K2055069
>BBa_K2055000
cttgaccttcccacaatggcaagctttaggctttctgattactagagaaagaggagaaatactagatgaatattagcga
tgtggcgaaaaaaaccggtttaaccagcaaagccattcggttttatgaagagaaagggctggtgacgccgccattacgt
agcgaaaatggttatcgcacctacacgcagaagcatttaaacgagctgacgttgctgcgacaggcgaggcaggtggggt
ttaatctggaagagtgtggcgaactggtcaatctgtttaacgatccgcgacgccatagcgcagatgtgaaaaagcggac
gctggaaaaggtcgcggagatcgagcgccatatcagcgagctgcaatcaatgcgcgatcaactgttggcgctggcggag
agttgtcccggcgatgacagcgccgactgcccgattattgataatctttccggctgctgtcatcacaaggcgcaaaagc
cacgttgatactagagaaagaggagaaatactagagattaaagaggagaaatactagatggcttcctccgaagacgtta
tcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtga
aggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatc
ctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcct
tcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccct
gcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaa
accatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctga
aactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgc
ttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaa
ggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata