Its organism of origin is Salmonella typhimurium.
DNA construct constituted by the pgolS promoter and the coding sequence of CueR. As the pgolS promoter has a basal transcription level, some CueR would be constantly expressed. CueR binds either copper or gold, though it is more selective for copper. CueR is a transcription factor that stimulates the transcription at the pgolS promoter when bound to copper/gold. Thus, this construct increases its expression in the presence of gold or copper through the production of a copper/gold sensitive protein.
The coding sequence is optimized for its expression in C. violaceum
Yili Natalia Padilla Flores, Jos?? Garc??a Lima, Marcelo ??lvarez Mata
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
cueR DNA-binding transcriptional regulator
Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
CueR binds either copper or gold, though it is more selective for copper. CueR is a transcription factor that stimulates the transcription at the pgolS promoter when bound to copper/gold.
The coding sequence is optimized for its expression in C. violaceum.
It is positively regulated by the transcription factor GolS. GolS induces transcription from this promoter once GolS is activated upon binding a gold ion.
This part was de novo synthesized by iGEM10_UIUC-Illinois. http://parts.igem.org/Part:BBa_K1127008
mRFP protein generator TERM+ RBS+(B0030)
Nicolo' Politi, Federica Sampietro, Davide Bianchini
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
Campbell et al., PNAS v99 p7877 URL
monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
RBS.1 (strong) -- modified from R. Weiss
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
Double terminator consisting of BBa_B0010 and BBa_B0012
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1