BBa_K2055000
type: ComponentDefinition
persistent identity: BBa K2055000
display id: BBa_K2055000
version: 1
was generated by: iGEM to SBOL conversion
description: Gold/copper inducible regulator
created: 2016-09-28T11:00:00Z
modified: 2016-10-19T08:24:26Z
mutable provenance: Its organism of origin is Salmonella typhimurium.
owned by: james
owned by: myers
mutable description: DNA construct constituted by the pgolS promoter and the coding sequence of CueR. As the pgolS promoter has a basal transcription level, some CueR would be constantly expressed. CueR binds either copper or gold, though it is more selective for copper. CueR is a transcription factor that stimulates the transcription at the pgolS promoter when bound to copper/gold. Thus, this construct increases its expression in the presence of gold or copper through the production of a copper/gold sensitive protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30723
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The coding sequence is optimized for its expression in C. violaceum
star: false
creator: Yili Natalia Padilla Flores, Jos?? Garc??a Lima, Marcelo ??lvarez Mata
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_K2055069
component: BBa_B0034
component: BBa_K2055169
component: BBa_K516030
sequence annotation: BBa_K2055169
sequence annotation: BBa_B0034
sequence annotation: BBa_K2055069
sequence annotation: BBa_K516030
sequence: https://synbiohub.org/public/igem/BBa_K2055000_sequence/1

BBa_K2055069
type: Component
persistent identity: component2523931
display id: component2523931
version: 1
top level: BBa_K2055000
definition: CueR
access: public

BBa_B0034
type: Component
persistent identity: component2523930
display id: component2523930
version: 1
top level: BBa_K2055000
definition: BBa_B0034
access: public

BBa_K2055169
type: Component
persistent identity: component2523928
display id: component2523928
version: 1
top level: BBa_K2055000
definition: BBa_K2055169
access: public

BBa_K516030
type: Component
persistent identity: component2523947
display id: component2523947
version: 1
top level: BBa_K2055000
definition: BBa_K516030
access: public

BBa_B0034
type: Component
persistent identity: component2523933
display id: component2523933
version: 1
top level: BBa_K2055000
definition: BBa_B0034
access: public

BBa_K2055169
type: SequenceAnnotation
persistent identity: annotation2523928
display id: annotation2523928
version: 1
top level: BBa_K2055000
location: https://synbiohub.org/public/igem/BBa_K2055000/annotation2523928/range2523928/1
component: BBa_K2055169

https://synbiohub.org/public/igem/BBa_K2055000/annotation2523928/range2523928/1
type: Range
persistent identity: range2523928
display id: range2523928
version: 1
top level: BBa_K2055000
direction: direction/reverse
start: 1
end: 39
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2523930
display id: annotation2523930
version: 1
top level: BBa_K2055000
location: https://synbiohub.org/public/igem/BBa_K2055000/annotation2523930/range2523930/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2055000/annotation2523930/range2523930/1
type: Range
persistent identity: range2523930
display id: range2523930
version: 1
top level: BBa_K2055000
direction: direction/reverse
start: 48
end: 59
orientation: inline

BBa_K2055069
type: SequenceAnnotation
persistent identity: annotation2523931
display id: annotation2523931
version: 1
top level: BBa_K2055000
location: https://synbiohub.org/public/igem/BBa_K2055000/annotation2523931/range2523931/1
component: BBa_K2055069

https://synbiohub.org/public/igem/BBa_K2055000/annotation2523931/range2523931/1
type: Range
persistent identity: range2523931
display id: range2523931
version: 1
top level: BBa_K2055000
direction: direction/reverse
start: 66
end: 482
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2523933
display id: annotation2523933
version: 1
top level: BBa_K2055000
location: https://synbiohub.org/public/igem/BBa_K2055000/annotation2523933/range2523933/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2055000/annotation2523933/range2523933/1
type: Range
persistent identity: range2523933
display id: range2523933
version: 1
top level: BBa_K2055000
direction: direction/reverse
start: 491
end: 502
orientation: inline

BBa_K516030
type: SequenceAnnotation
persistent identity: annotation2523947
display id: annotation2523947
version: 1
top level: BBa_K2055000
location: https://synbiohub.org/public/igem/BBa_K2055000/annotation2523947/range2523947/1
component: BBa_K516030

https://synbiohub.org/public/igem/BBa_K2055000/annotation2523947/range2523947/1
type: Range
persistent identity: range2523947
display id: range2523947
version: 1
top level: BBa_K2055000
direction: direction/reverse
start: 511
end: 1374
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

CueR
type: ComponentDefinition
persistent identity: BBa K2055069
display id: BBa_K2055069
version: 1
was generated by: iGEM to SBOL conversion
description: cueR DNA-binding transcriptional regulator
created: 2016-09-28T11:00:00Z
modified: 2016-09-29T04:34:44Z
mutable provenance: Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
owned by: james
owned by: myers
mutable description: CueR binds either copper or gold, though it is more selective for copper. CueR is a transcription factor that stimulates the transcription at the pgolS promoter when bound to copper/gold.
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30723
owner id: 30723
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The coding sequence is optimized for its expression in C. violaceum.
star: false
creator: Yili Natalia Padilla Flores
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2055069_sequence/1

BBa_K2055169
type: ComponentDefinition
persistent identity: BBa K2055169
display id: BBa_K2055169
version: 1
was generated by: iGEM to SBOL conversion
description: Gold inducible promoter
created: 2016-09-28T11:00:00Z
modified: 2016-09-29T05:35:57Z
mutable provenance: Salmonella typhimurium LT2
owned by: james
owned by: myers
mutable description: It is positively regulated by the transcription factor GolS. GolS induces transcription from this promoter once GolS is activated upon binding a gold ion.
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30723
owner id: 30723
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This part was de novo synthesized by iGEM10_UIUC-Illinois. http://parts.igem.org/Part:BBa_K1127008
star: false
creator: Yili Natalia Padilla Flores
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pGolS
sequence: https://synbiohub.org/public/igem/BBa_K2055169_sequence/1

pGolS
type: SequenceAnnotation
persistent identity: annotation2485449
display id: annotation2485449
version: 1
top level: BBa_K2055169
location: https://synbiohub.org/public/igem/BBa_K2055169/annotation2485449/range2485449/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K2055169/annotation2485449/range2485449/1
type: Range
persistent identity: range2485449
display id: range2485449
version: 1
top level: BBa_K2055169
direction: direction/forward
start: 1
end: 39
orientation: inline

BBa_K516030
type: ComponentDefinition
persistent identity: BBa K516030
display id: BBa_K516030
version: 1
was generated by: iGEM to SBOL conversion
description: mRFP protein generator TERM+ RBS+(B0030)
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:32Z
mutable provenance: Registry
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP protein generator TERM+ RBS+(B0030)
discontinued: false
dominant: false
experience: experience/Works
group u list: _681_
m user id: 0
owner id: 4150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: --
star: false
creator: Nicolo' Politi, Federica Sampietro, Davide Bianchini
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0015
component: BBa_E1010
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K516030_sequence/1

BBa_B0015
type: Component
persistent identity: component2250583
display id: component2250583
version: 1
top level: BBa_K516030
definition: BBa_B0015
access: public

BBa_E1010
type: Component
persistent identity: component2250576
display id: component2250576
version: 1
top level: BBa_K516030
definition: mRFP1
access: public

BBa_B0030
type: Component
persistent identity: component2250572
display id: component2250572
version: 1
top level: BBa_K516030
definition: BBa_B0030
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2250572
display id: annotation2250572
version: 1
top level: BBa_K516030
location: https://synbiohub.org/public/igem/BBa_K516030/annotation2250572/range2250572/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K516030/annotation2250572/range2250572/1
type: Range
persistent identity: range2250572
display id: range2250572
version: 1
top level: BBa_K516030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2250576
display id: annotation2250576
version: 1
top level: BBa_K516030
location: https://synbiohub.org/public/igem/BBa_K516030/annotation2250576/range2250576/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K516030/annotation2250576/range2250576/1
type: Range
persistent identity: range2250576
display id: range2250576
version: 1
top level: BBa_K516030
direction: direction/reverse
start: 22
end: 727
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2250583
display id: annotation2250583
version: 1
top level: BBa_K516030
location: https://synbiohub.org/public/igem/BBa_K516030/annotation2250583/range2250583/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K516030/annotation2250583/range2250583/1
type: Range
persistent identity: range2250583
display id: range2250583
version: 1
top level: BBa_K516030
direction: direction/reverse
start: 736
end: 864
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: BBa_B0030
sequence annotation: RBS-1\Strong
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K2055000_sequence/1
type: Sequence
persistent identity: BBa K2055000 sequence
display id: BBa_K2055000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttgaccttcccacaatggcaagctttaggctttctgattactagagaaagaggagaaatactagatgaatattagcgatgtggcgaaaaaaaccggtttaaccagcaaagccattcggttttatgaagagaaagggctggtgacgccgccattacgtagcgaaaatggttatcgcacctacacgcagaagcatttaaacgagctgacgttgctgcgacaggcgaggcaggtggggtttaatctggaagagtgtggcgaactggtcaatctgtttaacgatccgcgacgccatagcgcagatgtgaaaaagcggacgctggaaaaggtcgcggagatcgagcgccatatcagcgagctgcaatcaatgcgcgatcaactgttggcgctggcggagagttgtcccggcgatgacagcgccgactgcccgattattgataatctttccggctgctgtcatcacaaggcgcaaaagccacgttgatactagagaaagaggagaaatactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2055069_sequence/1
type: Sequence
persistent identity: BBa K2055069 sequence
display id: BBa_K2055069_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatattagcgatgtggcgaaaaaaaccggtttaaccagcaaagccattcggttttatgaagagaaagggctggtgacgccgccattacgtagcgaaaatggttatcgcacctacacgcagaagcatttaaacgagctgacgttgctgcgacaggcgaggcaggtggggtttaatctggaagagtgtggcgaactggtcaatctgtttaacgatccgcgacgccatagcgcagatgtgaaaaagcggacgctggaaaaggtcgcggagatcgagcgccatatcagcgagctgcaatcaatgcgcgatcaactgttggcgctggcggagagttgtcccggcgatgacagcgccgactgcccgattattgataatctttccggctgctgtcatcacaaggcgcaaaagccacgttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2055169_sequence/1
type: Sequence
persistent identity: BBa K2055169 sequence
display id: BBa_K2055169_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttgaccttcccacaatggcaagctttaggctttctgat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K516030_sequence/1
type: Sequence
persistent identity: BBa K516030 sequence
display id: BBa_K516030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z