LOCUS       BBa_K2055014            1749 bp    DNA     linear       16-Jun-2024
DEFINITION  Inverter with Ferric Uptake Regulator & LacI. Activates gene 
            expression in presence of iron.
ACCESSION   BBa_K2055014
VERSION     BBa_K2055014.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..498
                     /label=BBa_K2055009
     promoter        507..525
                     /label=BBa_K2055010
     misc_feature    538..541
                     /label=conserved
     RBS             534..545
                     /label=BBa_B0034
     CDS             552..1637
                     /label=BBa_K2055007
     stem_loop       1653..1672
                     /label=T7 TE
     misc_feature    1679..1679
                     /label=stop
     polyA_site      1673..1686
                     /label=polya
     misc_feature    1646..1686
                     /label=BBa_B0012
     terminator      1646..1686
                     /label=BBa_B0012
     misc_feature    1697..1713
                     /label=lac O1
     promoter        1714..1719
                     /label=-35
     misc_feature    1720..1736
                     /label=lac O1
     promoter        1737..1742
                     /label=-10
     misc_feature    1695..1748
                     /label=BBa_R0011
     promoter        1695..1748
                     /label=BBa_R0011
ORIGIN
        1 aaagaggaga aatactagat gattgatgag aggatgaact cggacgaact gaaacgggct
       61 ggtctcaagg ccaccctccc acgactcaag atcctacgga tattcgaaga ttcggatgct
      121 cgacatctga ccgcaggtga gatctatcgg ctcttgctgg aaaccggcga agaagtgggc
      181 ttggcgaccg tatatcgtgt actgacgcag ttcgagatgg caggtttggt ccgccgccat
      241 cattttgagg gcgataaggc cgtgttcgag ctgaacgaga ccggtcacca cgaccacatg
      301 gtgtgtactg cgtgtggaaa ggtgctcgag tttttcgacg aaatgctgga agcccggcaa
      361 cgcgagctcg ccgccaatcg cggtttcttc atttcggatc atagtctcta tctttatggc
      421 acctgtctgg gcatgcagga cgtgggaatc tgctcgcttc gcgacgacga cgcgccaggt
      481 gccagtactg actgataata ctagagtgta ataagactca ttcgttacta gagaaagagg
      541 agaaatacta gatgaaaccc gtaacgctct acgatgtggc ggaatacgct ggcgtgtctt
      601 accagaccgt cagcagggtt gtgaatcaag cctcccatgt atcggcgaaa acccgtgaga
      661 aagtggaggc ggcaatggct gaactgaact atatcccaaa ccgagttgct caacagctcg
      721 caggcaaaca gagtctcctc attggcgtcg ccacgtcctc gttggctctg catgccccga
      781 gccagattgt ggcggcgatc aagtcccgcg ccgaccagct tggagcgtcg gtggttgtct
      841 ctatggtgga acggtcaggc gttgaggcgt gcaaggctgc ggtacataac ctgctggccc
      901 agcgagtaag cggcctcatt attaactacc ctctggacga tcaggacgct atcgctgtcg
      961 aggccgcatg tacaaacgtc cccgccctgt tcctcgacgt ctcggatcag acgcccatca
     1021 acagtataat tttttctcat gaagacggca cccggttagg cgttgagcat ctggtagcct
     1081 tgggccacca gcagattgcc ctcctggcag gccctctttc gagtgtaagc gcccgcttgc
     1141 gccttgcagg ctggcataag tatcttaccc gaaaccagat ccagccgatc gcggaacgcg
     1201 agggagattg gtccgccatg tcaggatttc aacagacgat gcaaatgctc aacgaaggta
     1261 tcgtaccgac ggccatgttg gtcgcgaacg accaaatggc cctgggcgcg atgcgggcga
     1321 taacggaaag cggcctacgc gtcggagccg acatctcagt ggtcggctac gacgatacgg
     1381 aagactcctc ctgctatatt ccccccctca cgaccattaa gcaggacttt cgcctgttgg
     1441 gacaaacgtc cgttgatcga ctccttcagc tgtcgcaggg tcaagccgtg aaaggaaatc
     1501 agctcctgcc tgtcagtctg gtgaaacgca agaccacctt ggcccccaat acccaaaccg
     1561 ccagcccccg cgcgcttgcg gattctctca tgcagttggc tagacaggtt agtcgcctgg
     1621 aatcgggaca gtgataatac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1681 tttatatact agagaattgt gagcggataa caattgacat tgtgagcgga taacaagata
     1741 ctgagcaca
//