LOCUS       BBa_K2055015            1867 bp    DNA     linear       22-Dec-2024
DEFINITION  Promoter + Inverter with Ferric Uptake Regulator & LacI. Activates 
            gene expression in presence of Fe
ACCESSION   BBa_K2055015
VERSION     BBa_K2055015.1  
FEATURES             Location/Qualifiers
     promoter        1..89
                     /label=BBa_K2055013
     RBS             98..110
                     /label=RBS-3\Weak
     RBS             104..107
                     /label=RBS
     misc_feature    98..110
                     /label=BBa_B0032
     RBS             98..110
                     /label=BBa_B0032
     misc_feature    123..126
                     /label=conserved
     RBS             119..130
                     /label=BBa_B0034
     CDS             137..616
                     /label=BBa_K2055009
     promoter        625..643
                     /label=BBa_K2055010
     misc_feature    656..659
                     /label=conserved
     RBS             652..663
                     /label=BBa_B0034
     CDS             670..1755
                     /label=BBa_K2055007
     stem_loop       1771..1790
                     /label=T7 TE
     misc_feature    1797..1797
                     /label=stop
     polyA_site      1791..1804
                     /label=polya
     misc_feature    1764..1804
                     /label=BBa_B0012
     terminator      1764..1804
                     /label=BBa_B0012
     misc_feature    1815..1831
                     /label=lac O1
     promoter        1832..1837
                     /label=-35
     misc_feature    1838..1854
                     /label=lac O1
     promoter        1855..1860
                     /label=-10
     misc_feature    1813..1866
                     /label=BBa_R0011
     promoter        1813..1866
                     /label=BBa_R0011
ORIGIN
        1 actcgtaccc ataacgttgc atgccagagt ccaggcgtac agtcgcttgg ctggcgtcct
       61 attgatgccc gtgaaggagg tgtaaaatgt actagagtca cacaggaaag tactagagaa
      121 agaggagaaa tactagatga ttgatgagag gatgaactcg gacgaactga aacgggctgg
      181 tctcaaggcc accctcccac gactcaagat cctacggata ttcgaagatt cggatgctcg
      241 acatctgacc gcaggtgaga tctatcggct cttgctggaa accggcgaag aagtgggctt
      301 ggcgaccgta tatcgtgtac tgacgcagtt cgagatggca ggtttggtcc gccgccatca
      361 ttttgagggc gataaggccg tgttcgagct gaacgagacc ggtcaccacg accacatggt
      421 gtgtactgcg tgtggaaagg tgctcgagtt tttcgacgaa atgctggaag cccggcaacg
      481 cgagctcgcc gccaatcgcg gtttcttcat ttcggatcat agtctctatc tttatggcac
      541 ctgtctgggc atgcaggacg tgggaatctg ctcgcttcgc gacgacgacg cgccaggtgc
      601 cagtactgac tgataatact agagtgtaat aagactcatt cgttactaga gaaagaggag
      661 aaatactaga tgaaacccgt aacgctctac gatgtggcgg aatacgctgg cgtgtcttac
      721 cagaccgtca gcagggttgt gaatcaagcc tcccatgtat cggcgaaaac ccgtgagaaa
      781 gtggaggcgg caatggctga actgaactat atcccaaacc gagttgctca acagctcgca
      841 ggcaaacaga gtctcctcat tggcgtcgcc acgtcctcgt tggctctgca tgccccgagc
      901 cagattgtgg cggcgatcaa gtcccgcgcc gaccagcttg gagcgtcggt ggttgtctct
      961 atggtggaac ggtcaggcgt tgaggcgtgc aaggctgcgg tacataacct gctggcccag
     1021 cgagtaagcg gcctcattat taactaccct ctggacgatc aggacgctat cgctgtcgag
     1081 gccgcatgta caaacgtccc cgccctgttc ctcgacgtct cggatcagac gcccatcaac
     1141 agtataattt tttctcatga agacggcacc cggttaggcg ttgagcatct ggtagccttg
     1201 ggccaccagc agattgccct cctggcaggc cctctttcga gtgtaagcgc ccgcttgcgc
     1261 cttgcaggct ggcataagta tcttacccga aaccagatcc agccgatcgc ggaacgcgag
     1321 ggagattggt ccgccatgtc aggatttcaa cagacgatgc aaatgctcaa cgaaggtatc
     1381 gtaccgacgg ccatgttggt cgcgaacgac caaatggccc tgggcgcgat gcgggcgata
     1441 acggaaagcg gcctacgcgt cggagccgac atctcagtgg tcggctacga cgatacggaa
     1501 gactcctcct gctatattcc ccccctcacg accattaagc aggactttcg cctgttggga
     1561 caaacgtccg ttgatcgact ccttcagctg tcgcagggtc aagccgtgaa aggaaatcag
     1621 ctcctgcctg tcagtctggt gaaacgcaag accaccttgg cccccaatac ccaaaccgcc
     1681 agcccccgcg cgcttgcgga ttctctcatg cagttggcta gacaggttag tcgcctggaa
     1741 tcgggacagt gataatacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1801 tatatactag agaattgtga gcggataaca attgacattg tgagcggata acaagatact
     1861 gagcaca
//