BBa_K2055015
type: ComponentDefinition
persistent identity: BBa K2055015
display id: BBa_K2055015
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter + Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of Fe
created: 2016-09-27T11:00:00Z
modified: 2016-10-14T08:58:35Z
mutable provenance: Composite part
owned by: james
owned by: myers
mutable description: E. coli constitutive promoter with Ferric Uptake Regulator & LacI Inverter. Activates gene expression in presence of iron.
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30725
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: LacI and FUR proteins are optimized for Acidithiobacillus ferrooxidans. It has two RBS after the constitutive promoter and before the FUR CDS.
star: false
creator: Raul Garza & Carlos Vasquez
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0012
component: BBa_K2055007
component: BBa_R0011
component: BBa_K2055009
component: BBa_B0034
component: BBa_K2055010
component: BBa_B0032
component: BBa_K2055013
sequence annotation: BBa_K2055013
sequence annotation: BBa_B0032
sequence annotation: BBa_B0034
sequence annotation: BBa_K2055009
sequence annotation: BBa_K2055010
sequence annotation: BBa_K2055007
sequence annotation: BBa_B0012
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_K2055015_sequence/1

BBa_B0012
type: Component
persistent identity: component2511684
display id: component2511684
version: 1
top level: BBa_K2055015
definition: BBa_B0012
access: public

BBa_K2055007
type: Component
persistent identity: component2511683
display id: component2511683
version: 1
top level: BBa_K2055015
definition: BBa_K2055007
access: public

BBa_R0011
type: Component
persistent identity: component2511688
display id: component2511688
version: 1
top level: BBa_K2055015
definition: lacI+pL
access: public

BBa_K2055009
type: Component
persistent identity: component2511679
display id: component2511679
version: 1
top level: BBa_K2055015
definition: BBa_K2055009
access: public

BBa_B0034
type: Component
persistent identity: component2511678
display id: component2511678
version: 1
top level: BBa_K2055015
definition: BBa_B0034
access: public

BBa_B0034
type: Component
persistent identity: component2511682
display id: component2511682
version: 1
top level: BBa_K2055015
definition: BBa_B0034
access: public

BBa_K2055010
type: Component
persistent identity: component2511680
display id: component2511680
version: 1
top level: BBa_K2055015
definition: BBa_K2055010
access: public

BBa_B0032
type: Component
persistent identity: component2511675
display id: component2511675
version: 1
top level: BBa_K2055015
definition: BBa_B0032
access: public

BBa_K2055013
type: Component
persistent identity: component2511673
display id: component2511673
version: 1
top level: BBa_K2055015
definition: BBa_K2055013
access: public

BBa_K2055013
type: SequenceAnnotation
persistent identity: annotation2511673
display id: annotation2511673
version: 1
top level: BBa_K2055015
location: https://synbiohub.org/public/igem/BBa_K2055015/annotation2511673/range2511673/1
component: BBa_K2055013

https://synbiohub.org/public/igem/BBa_K2055015/annotation2511673/range2511673/1
type: Range
persistent identity: range2511673
display id: range2511673
version: 1
top level: BBa_K2055015
direction: direction/reverse
start: 1
end: 89
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2511675
display id: annotation2511675
version: 1
top level: BBa_K2055015
location: https://synbiohub.org/public/igem/BBa_K2055015/annotation2511675/range2511675/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K2055015/annotation2511675/range2511675/1
type: Range
persistent identity: range2511675
display id: range2511675
version: 1
top level: BBa_K2055015
direction: direction/forward
start: 98
end: 110
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2511678
display id: annotation2511678
version: 1
top level: BBa_K2055015
location: https://synbiohub.org/public/igem/BBa_K2055015/annotation2511678/range2511678/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2055015/annotation2511678/range2511678/1
type: Range
persistent identity: range2511678
display id: range2511678
version: 1
top level: BBa_K2055015
direction: direction/reverse
start: 119
end: 130
orientation: inline

BBa_K2055009
type: SequenceAnnotation
persistent identity: annotation2511679
display id: annotation2511679
version: 1
top level: BBa_K2055015
location: https://synbiohub.org/public/igem/BBa_K2055015/annotation2511679/range2511679/1
component: BBa_K2055009

https://synbiohub.org/public/igem/BBa_K2055015/annotation2511679/range2511679/1
type: Range
persistent identity: range2511679
display id: range2511679
version: 1
top level: BBa_K2055015
direction: direction/reverse
start: 137
end: 616
orientation: inline

BBa_K2055010
type: SequenceAnnotation
persistent identity: annotation2511680
display id: annotation2511680
version: 1
top level: BBa_K2055015
location: https://synbiohub.org/public/igem/BBa_K2055015/annotation2511680/range2511680/1
component: BBa_K2055010

https://synbiohub.org/public/igem/BBa_K2055015/annotation2511680/range2511680/1
type: Range
persistent identity: range2511680
display id: range2511680
version: 1
top level: BBa_K2055015
direction: direction/reverse
start: 625
end: 643
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2511682
display id: annotation2511682
version: 1
top level: BBa_K2055015
location: https://synbiohub.org/public/igem/BBa_K2055015/annotation2511682/range2511682/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2055015/annotation2511682/range2511682/1
type: Range
persistent identity: range2511682
display id: range2511682
version: 1
top level: BBa_K2055015
direction: direction/reverse
start: 652
end: 663
orientation: inline

BBa_K2055007
type: SequenceAnnotation
persistent identity: annotation2511683
display id: annotation2511683
version: 1
top level: BBa_K2055015
location: https://synbiohub.org/public/igem/BBa_K2055015/annotation2511683/range2511683/1
component: BBa_K2055007

https://synbiohub.org/public/igem/BBa_K2055015/annotation2511683/range2511683/1
type: Range
persistent identity: range2511683
display id: range2511683
version: 1
top level: BBa_K2055015
direction: direction/reverse
start: 670
end: 1755
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2511684
display id: annotation2511684
version: 1
top level: BBa_K2055015
location: https://synbiohub.org/public/igem/BBa_K2055015/annotation2511684/range2511684/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K2055015/annotation2511684/range2511684/1
type: Range
persistent identity: range2511684
display id: range2511684
version: 1
top level: BBa_K2055015
direction: direction/forward
start: 1764
end: 1804
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2511688
display id: annotation2511688
version: 1
top level: BBa_K2055015
location: https://synbiohub.org/public/igem/BBa_K2055015/annotation2511688/range2511688/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K2055015/annotation2511688/range2511688/1
type: Range
persistent identity: range2511688
display id: range2511688
version: 1
top level: BBa_K2055015
direction: direction/forward
start: 1813
end: 1866
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K2055007
type: ComponentDefinition
persistent identity: BBa K2055007
display id: BBa_K2055007
version: 1
was generated by: iGEM to SBOL conversion
description: LacI for Acidithiobacillus
created: 2016-09-27T11:00:00Z
modified: 2016-09-28T06:11:09Z
mutable provenance: The RBS used is the BBa_B0034, the terminator is the BBA_B0012, the LacI reculated Promoter is the BBa_R0011. The sequence for the FUR Box was obtained from the results of the paper of Quatrini et al. (Citation in our wiki), choosing the Fur box with the best score. The sequence for the FUR protein was obtained from the Acidithiobacillus ferrooxidans genome.
owned by: james
owned by: myers
mutable description: Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of iron. LacI and FUR proteins are optimized for Acidithiobacillus ferrooxidans.
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30725
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: It does not have a promoter.
star: false
creator: Carmen Padilla, Melissa Gonzalez & Melissa Rios
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2055007_sequence/1

BBa_K2055009
type: ComponentDefinition
persistent identity: BBa K2055009
display id: BBa_K2055009
version: 1
was generated by: iGEM to SBOL conversion
description: FUR Protein
created: 2016-09-27T11:00:00Z
modified: 2016-09-28T04:06:55Z
mutable provenance: The sequence for the FUR Box was obtained from the results of the paper of Quatrini et al. (Citation in our wiki).
owned by: james
owned by: myers
mutable description: Ferric Uptace Regulator Protein codon-optimized for Acidithiobacillus Ferrooxidans, the FUR Protein in presence of iron will block the transcription because it will bind to its FUR Box.
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30725
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: You will have to add a FUR Box to repress the transcription with this FUR protein in presence of iron.
star: false
creator: Carlos Vasquez
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2055009_sequence/1

BBa_K2055010
type: ComponentDefinition
persistent identity: BBa K2055010
display id: BBa_K2055010
version: 1
was generated by: iGEM to SBOL conversion
description: FUR Box
created: 2016-09-27T11:00:00Z
modified: 2016-09-28T04:07:16Z
mutable provenance: It is the FUR Box with the highest score in the work of: Quatrini, R., Lefimil, C., Veloso, F. A., Pedroso, I., Holmes, D. S., & Jedlicki, E. (2007). Bioinformatic prediction and experimental verification of Fur-regulated genes in the extreme acidophile Acidithiobacillus ferrooxidans. Nucleic Acids Research, 35(7), 2153???2166. http://doi.org/10.1093/nar/gkm068
owned by: james
owned by: myers
mutable description: This is a FUR Box with high affinity to BBa_K2055009. In presence of iron, it will block the downstream transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30725
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: It needs iron to block transcription.
star: false
creator: Carlos Vasquez
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K2055010_sequence/1

BBa_K2055013
type: ComponentDefinition
persistent identity: BBa K2055013
display id: BBa_K2055013
version: 1
was generated by: iGEM to SBOL conversion
description: Heat Shock Promoter
created: 2016-09-27T11:00:00Z
modified: 2016-09-28T04:19:49Z
mutable provenance: Ribeiro, D. A., Del Bem, L. E., Vicentini, R., Ferraz, L. F., Murakami, M. T., & Ottoboni, L. M. (2011). The small heat shock proteins from Acidithiobacillus ferrooxidans: gene expression, phylogenetic analysis, and structural modeling. BMC microbiology, 11(1), 1.
owned by: james
owned by: myers
mutable description: σ32-dependent promoter for for the Afe_1437 gene in Acidithiobacillus ferrooxidans
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30725
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Putative promoter in Acidithiobacillus ferrooxidans. Must check its characterization.
star: false
creator: Raul Garza
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K2055013_sequence/1

https://synbiohub.org/public/igem/BBa_K2055015_sequence/1
type: Sequence
persistent identity: BBa K2055015 sequence
display id: BBa_K2055015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actcgtacccataacgttgcatgccagagtccaggcgtacagtcgcttggctggcgtcctattgatgcccgtgaaggaggtgtaaaatgtactagagtcacacaggaaagtactagagaaagaggagaaatactagatgattgatgagaggatgaactcggacgaactgaaacgggctggtctcaaggccaccctcccacgactcaagatcctacggatattcgaagattcggatgctcgacatctgaccgcaggtgagatctatcggctcttgctggaaaccggcgaagaagtgggcttggcgaccgtatatcgtgtactgacgcagttcgagatggcaggtttggtccgccgccatcattttgagggcgataaggccgtgttcgagctgaacgagaccggtcaccacgaccacatggtgtgtactgcgtgtggaaaggtgctcgagtttttcgacgaaatgctggaagcccggcaacgcgagctcgccgccaatcgcggtttcttcatttcggatcatagtctctatctttatggcacctgtctgggcatgcaggacgtgggaatctgctcgcttcgcgacgacgacgcgccaggtgccagtactgactgataatactagagtgtaataagactcattcgttactagagaaagaggagaaatactagatgaaacccgtaacgctctacgatgtggcggaatacgctggcgtgtcttaccagaccgtcagcagggttgtgaatcaagcctcccatgtatcggcgaaaacccgtgagaaagtggaggcggcaatggctgaactgaactatatcccaaaccgagttgctcaacagctcgcaggcaaacagagtctcctcattggcgtcgccacgtcctcgttggctctgcatgccccgagccagattgtggcggcgatcaagtcccgcgccgaccagcttggagcgtcggtggttgtctctatggtggaacggtcaggcgttgaggcgtgcaaggctgcggtacataacctgctggcccagcgagtaagcggcctcattattaactaccctctggacgatcaggacgctatcgctgtcgaggccgcatgtacaaacgtccccgccctgttcctcgacgtctcggatcagacgcccatcaacagtataattttttctcatgaagacggcacccggttaggcgttgagcatctggtagccttgggccaccagcagattgccctcctggcaggccctctttcgagtgtaagcgcccgcttgcgccttgcaggctggcataagtatcttacccgaaaccagatccagccgatcgcggaacgcgagggagattggtccgccatgtcaggatttcaacagacgatgcaaatgctcaacgaaggtatcgtaccgacggccatgttggtcgcgaacgaccaaatggccctgggcgcgatgcgggcgataacggaaagcggcctacgcgtcggagccgacatctcagtggtcggctacgacgatacggaagactcctcctgctatattccccccctcacgaccattaagcaggactttcgcctgttgggacaaacgtccgttgatcgactccttcagctgtcgcagggtcaagccgtgaaaggaaatcagctcctgcctgtcagtctggtgaaacgcaagaccaccttggcccccaatacccaaaccgccagcccccgcgcgcttgcggattctctcatgcagttggctagacaggttagtcgcctggaatcgggacagtgataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2055007_sequence/1
type: Sequence
persistent identity: BBa K2055007 sequence
display id: BBa_K2055007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaacccgtaacgctctacgatgtggcggaatacgctggcgtgtcttaccagaccgtcagcagggttgtgaatcaagcctcccatgtatcggcgaaaacccgtgagaaagtggaggcggcaatggctgaactgaactatatcccaaaccgagttgctcaacagctcgcaggcaaacagagtctcctcattggcgtcgccacgtcctcgttggctctgcatgccccgagccagattgtggcggcgatcaagtcccgcgccgaccagcttggagcgtcggtggttgtctctatggtggaacggtcaggcgttgaggcgtgcaaggctgcggtacataacctgctggcccagcgagtaagcggcctcattattaactaccctctggacgatcaggacgctatcgctgtcgaggccgcatgtacaaacgtccccgccctgttcctcgacgtctcggatcagacgcccatcaacagtataattttttctcatgaagacggcacccggttaggcgttgagcatctggtagccttgggccaccagcagattgccctcctggcaggccctctttcgagtgtaagcgcccgcttgcgccttgcaggctggcataagtatcttacccgaaaccagatccagccgatcgcggaacgcgagggagattggtccgccatgtcaggatttcaacagacgatgcaaatgctcaacgaaggtatcgtaccgacggccatgttggtcgcgaacgaccaaatggccctgggcgcgatgcgggcgataacggaaagcggcctacgcgtcggagccgacatctcagtggtcggctacgacgatacggaagactcctcctgctatattccccccctcacgaccattaagcaggactttcgcctgttgggacaaacgtccgttgatcgactccttcagctgtcgcagggtcaagccgtgaaaggaaatcagctcctgcctgtcagtctggtgaaacgcaagaccaccttggcccccaatacccaaaccgccagcccccgcgcgcttgcggattctctcatgcagttggctagacaggttagtcgcctggaatcgggacagtgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2055009_sequence/1
type: Sequence
persistent identity: BBa K2055009 sequence
display id: BBa_K2055009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgatgagaggatgaactcggacgaactgaaacgggctggtctcaaggccaccctcccacgactcaagatcctacggatattcgaagattcggatgctcgacatctgaccgcaggtgagatctatcggctcttgctggaaaccggcgaagaagtgggcttggcgaccgtatatcgtgtactgacgcagttcgagatggcaggtttggtccgccgccatcattttgagggcgataaggccgtgttcgagctgaacgagaccggtcaccacgaccacatggtgtgtactgcgtgtggaaaggtgctcgagtttttcgacgaaatgctggaagcccggcaacgcgagctcgccgccaatcgcggtttcttcatttcggatcatagtctctatctttatggcacctgtctgggcatgcaggacgtgggaatctgctcgcttcgcgacgacgacgcgccaggtgccagtactgactgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2055010_sequence/1
type: Sequence
persistent identity: BBa K2055010 sequence
display id: BBa_K2055010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtaataagactcattcgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2055013_sequence/1
type: Sequence
persistent identity: BBa K2055013 sequence
display id: BBa_K2055013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: actcgtacccataacgttgcatgccagagtccaggcgtacagtcgcttggctggcgtcctattgatgcccgtgaaggaggtgtaaaatg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z