LOCUS       BBa_K2055016            2682 bp    DNA     linear       22-Dec-2024
DEFINITION  Part designed for verification and characterization of the 
            FUR/LacI inverter
ACCESSION   BBa_K2055016
VERSION     BBa_K2055016.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    69..72
                     /label=conserved
     RBS             65..76
                     /label=BBa_B0034
     CDS             83..562
                     /label=BBa_K2055009
     promoter        571..589
                     /label=BBa_K2055010
     misc_feature    602..605
                     /label=conserved
     RBS             598..609
                     /label=BBa_B0034
     CDS             616..1701
                     /label=BBa_K2055007
     stem_loop       1717..1736
                     /label=T7 TE
     misc_feature    1743..1743
                     /label=stop
     polyA_site      1737..1750
                     /label=polya
     misc_feature    1710..1750
                     /label=BBa_B0012
     terminator      1710..1750
                     /label=BBa_B0012
     misc_feature    1761..1777
                     /label=lac O1
     promoter        1778..1783
                     /label=-35
     misc_feature    1784..1800
                     /label=lac O1
     promoter        1801..1806
                     /label=-10
     misc_feature    1759..1812
                     /label=BBa_R0011
     promoter        1759..1812
                     /label=BBa_R0011
     misc_feature    1826..1829
                     /label=conserved
     RBS             1822..1833
                     /label=BBa_B0034
     CDS             1840..2514
                     /label=mrfp1
     misc_feature    2521..2545
                     /label=Help:Barcodes
     CDS             1840..2545
                     /label=BBa_E1010
     stem_loop       2565..2608
                     /label=stem_loop
     misc_feature    2554..2633
                     /label=BBa_B0010
     terminator      2554..2633
                     /label=BBa_B0010
     stem_loop       2649..2668
                     /label=T7 TE
     misc_feature    2675..2675
                     /label=stop
     polyA_site      2669..2682
                     /label=polya
     misc_feature    2642..2682
                     /label=BBa_B0012
     terminator      2642..2682
                     /label=BBa_B0012
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagtcacaca ggaaagtact
       61 agagaaagag gagaaatact agatgattga tgagaggatg aactcggacg aactgaaacg
      121 ggctggtctc aaggccaccc tcccacgact caagatccta cggatattcg aagattcgga
      181 tgctcgacat ctgaccgcag gtgagatcta tcggctcttg ctggaaaccg gcgaagaagt
      241 gggcttggcg accgtatatc gtgtactgac gcagttcgag atggcaggtt tggtccgccg
      301 ccatcatttt gagggcgata aggccgtgtt cgagctgaac gagaccggtc accacgacca
      361 catggtgtgt actgcgtgtg gaaaggtgct cgagtttttc gacgaaatgc tggaagcccg
      421 gcaacgcgag ctcgccgcca atcgcggttt cttcatttcg gatcatagtc tctatcttta
      481 tggcacctgt ctgggcatgc aggacgtggg aatctgctcg cttcgcgacg acgacgcgcc
      541 aggtgccagt actgactgat aatactagag tgtaataaga ctcattcgtt actagagaaa
      601 gaggagaaat actagatgaa acccgtaacg ctctacgatg tggcggaata cgctggcgtg
      661 tcttaccaga ccgtcagcag ggttgtgaat caagcctccc atgtatcggc gaaaacccgt
      721 gagaaagtgg aggcggcaat ggctgaactg aactatatcc caaaccgagt tgctcaacag
      781 ctcgcaggca aacagagtct cctcattggc gtcgccacgt cctcgttggc tctgcatgcc
      841 ccgagccaga ttgtggcggc gatcaagtcc cgcgccgacc agcttggagc gtcggtggtt
      901 gtctctatgg tggaacggtc aggcgttgag gcgtgcaagg ctgcggtaca taacctgctg
      961 gcccagcgag taagcggcct cattattaac taccctctgg acgatcagga cgctatcgct
     1021 gtcgaggccg catgtacaaa cgtccccgcc ctgttcctcg acgtctcgga tcagacgccc
     1081 atcaacagta taattttttc tcatgaagac ggcacccggt taggcgttga gcatctggta
     1141 gccttgggcc accagcagat tgccctcctg gcaggccctc tttcgagtgt aagcgcccgc
     1201 ttgcgccttg caggctggca taagtatctt acccgaaacc agatccagcc gatcgcggaa
     1261 cgcgagggag attggtccgc catgtcagga tttcaacaga cgatgcaaat gctcaacgaa
     1321 ggtatcgtac cgacggccat gttggtcgcg aacgaccaaa tggccctggg cgcgatgcgg
     1381 gcgataacgg aaagcggcct acgcgtcgga gccgacatct cagtggtcgg ctacgacgat
     1441 acggaagact cctcctgcta tattcccccc ctcacgacca ttaagcagga ctttcgcctg
     1501 ttgggacaaa cgtccgttga tcgactcctt cagctgtcgc agggtcaagc cgtgaaagga
     1561 aatcagctcc tgcctgtcag tctggtgaaa cgcaagacca ccttggcccc caatacccaa
     1621 accgccagcc cccgcgcgct tgcggattct ctcatgcagt tggctagaca ggttagtcgc
     1681 ctggaatcgg gacagtgata atactagagt cacactggct caccttcggg tgggcctttc
     1741 tgcgtttata tactagagaa ttgtgagcgg ataacaattg acattgtgag cggataacaa
     1801 gatactgagc acatactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt
     1861 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc
     1921 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa
     1981 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac
     2041 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc
     2101 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt
     2161 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc
     2221 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc
     2281 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg
     2341 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt
     2401 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac
     2461 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa
     2521 cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc
     2581 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     2641 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//