LOCUS       BBa_K2055019            2715 bp    DNA     linear       09-May-2024
DEFINITION  Part designed for verification and characterization of the heat 
            the shock promoter and Inverter.
ACCESSION   BBa_K2055019
VERSION     BBa_K2055019.1  
FEATURES             Location/Qualifiers
     promoter        1..89
                     /label=BBa_K2055013
     misc_feature    102..105
                     /label=conserved
     RBS             98..109
                     /label=BBa_B0034
     CDS             116..595
                     /label=BBa_K2055009
     promoter        604..622
                     /label=BBa_K2055010
     misc_feature    635..638
                     /label=conserved
     RBS             631..642
                     /label=BBa_B0034
     CDS             649..1734
                     /label=BBa_K2055007
     stem_loop       1750..1769
                     /label=T7 TE
     misc_feature    1776..1776
                     /label=stop
     polyA_site      1770..1783
                     /label=polya
     misc_feature    1743..1783
                     /label=BBa_B0012
     terminator      1743..1783
                     /label=BBa_B0012
     misc_feature    1794..1810
                     /label=lac O1
     promoter        1811..1816
                     /label=-35
     misc_feature    1817..1833
                     /label=lac O1
     promoter        1834..1839
                     /label=-10
     misc_feature    1792..1845
                     /label=BBa_R0011
     promoter        1792..1845
                     /label=BBa_R0011
     misc_feature    1859..1862
                     /label=conserved
     RBS             1855..1866
                     /label=BBa_B0034
     CDS             1873..2547
                     /label=mrfp1
     misc_feature    2554..2578
                     /label=Help:Barcodes
     CDS             1873..2578
                     /label=BBa_E1010
     stem_loop       2598..2641
                     /label=stem_loop
     misc_feature    2587..2666
                     /label=BBa_B0010
     terminator      2587..2666
                     /label=BBa_B0010
     stem_loop       2682..2701
                     /label=T7 TE
     misc_feature    2708..2708
                     /label=stop
     polyA_site      2702..2715
                     /label=polya
     misc_feature    2675..2715
                     /label=BBa_B0012
     terminator      2675..2715
                     /label=BBa_B0012
ORIGIN
        1 actcgtaccc ataacgttgc atgccagagt ccaggcgtac agtcgcttgg ctggcgtcct
       61 attgatgccc gtgaaggagg tgtaaaatgt actagagaaa gaggagaaat actagatgat
      121 tgatgagagg atgaactcgg acgaactgaa acgggctggt ctcaaggcca ccctcccacg
      181 actcaagatc ctacggatat tcgaagattc ggatgctcga catctgaccg caggtgagat
      241 ctatcggctc ttgctggaaa ccggcgaaga agtgggcttg gcgaccgtat atcgtgtact
      301 gacgcagttc gagatggcag gtttggtccg ccgccatcat tttgagggcg ataaggccgt
      361 gttcgagctg aacgagaccg gtcaccacga ccacatggtg tgtactgcgt gtggaaaggt
      421 gctcgagttt ttcgacgaaa tgctggaagc ccggcaacgc gagctcgccg ccaatcgcgg
      481 tttcttcatt tcggatcata gtctctatct ttatggcacc tgtctgggca tgcaggacgt
      541 gggaatctgc tcgcttcgcg acgacgacgc gccaggtgcc agtactgact gataatacta
      601 gagtgtaata agactcattc gttactagag aaagaggaga aatactagat gaaacccgta
      661 acgctctacg atgtggcgga atacgctggc gtgtcttacc agaccgtcag cagggttgtg
      721 aatcaagcct cccatgtatc ggcgaaaacc cgtgagaaag tggaggcggc aatggctgaa
      781 ctgaactata tcccaaaccg agttgctcaa cagctcgcag gcaaacagag tctcctcatt
      841 ggcgtcgcca cgtcctcgtt ggctctgcat gccccgagcc agattgtggc ggcgatcaag
      901 tcccgcgccg accagcttgg agcgtcggtg gttgtctcta tggtggaacg gtcaggcgtt
      961 gaggcgtgca aggctgcggt acataacctg ctggcccagc gagtaagcgg cctcattatt
     1021 aactaccctc tggacgatca ggacgctatc gctgtcgagg ccgcatgtac aaacgtcccc
     1081 gccctgttcc tcgacgtctc ggatcagacg cccatcaaca gtataatttt ttctcatgaa
     1141 gacggcaccc ggttaggcgt tgagcatctg gtagccttgg gccaccagca gattgccctc
     1201 ctggcaggcc ctctttcgag tgtaagcgcc cgcttgcgcc ttgcaggctg gcataagtat
     1261 cttacccgaa accagatcca gccgatcgcg gaacgcgagg gagattggtc cgccatgtca
     1321 ggatttcaac agacgatgca aatgctcaac gaaggtatcg taccgacggc catgttggtc
     1381 gcgaacgacc aaatggccct gggcgcgatg cgggcgataa cggaaagcgg cctacgcgtc
     1441 ggagccgaca tctcagtggt cggctacgac gatacggaag actcctcctg ctatattccc
     1501 cccctcacga ccattaagca ggactttcgc ctgttgggac aaacgtccgt tgatcgactc
     1561 cttcagctgt cgcagggtca agccgtgaaa ggaaatcagc tcctgcctgt cagtctggtg
     1621 aaacgcaaga ccaccttggc ccccaatacc caaaccgcca gcccccgcgc gcttgcggat
     1681 tctctcatgc agttggctag acaggttagt cgcctggaat cgggacagtg ataatactag
     1741 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag
     1801 cggataacaa ttgacattgt gagcggataa caagatactg agcacatact agagaaagag
     1861 gagaaatact agatggcttc ctccgaagac gttatcaaag agttcatgcg tttcaaagtt
     1921 cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt
     1981 ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc
     2041 gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg
     2101 gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt
     2161 atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt
     2221 gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg
     2281 cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct
     2341 ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa
     2401 gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac
     2461 atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt
     2521 gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg tagatcgcta
     2581 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2641 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     2701 ctttctgcgt ttata
//