##gff-version 3
##sequence-region BBa_K2055019 1 2715
BBa_K2055019	.	terminator	1743	1783	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K2055019	.	engineered_region	1743	1783	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2055019	.	stop_codon	1776	1776	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2055019	.	stem_loop	1750	1769	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2055019	.	polyA_site	1770	1783	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2055019	.	terminator	2675	2715	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K2055019	.	engineered_region	2675	2715	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2055019	.	stop_codon	2708	2708	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2055019	.	stem_loop	2682	2701	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2055019	.	polyA_site	2702	2715	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2055019	.	CDS	116	595	.	+	0	ID=BBa_K2055009;Name=BBa_K2055009
BBa_K2055019	.	CDS	1873	2578	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K2055019	.	engineered_tag	2554	2578	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K2055019	.	CDS	1873	2547	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K2055019	.	ribosome_entry_site	631	642	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2055019	.	conserved_region	635	638	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2055019	.	ribosome_entry_site	1855	1866	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2055019	.	conserved_region	1859	1862	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2055019	.	promoter	604	622	.	+	0	ID=BBa_K2055010;Name=BBa_K2055010
BBa_K2055019	.	terminator	2587	2666	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K2055019	.	engineered_region	2587	2666	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2055019	.	stem_loop	2598	2641	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2055019	.	ribosome_entry_site	98	109	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2055019	.	conserved_region	102	105	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2055019	.	promoter	1	89	.	+	0	ID=BBa_K2055013;Name=BBa_K2055013
BBa_K2055019	.	CDS	649	1734	.	+	0	ID=BBa_K2055007;Name=BBa_K2055007
BBa_K2055019	.	promoter	1792	1845	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K2055019	.	promoter	1834	1839	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K2055019	.	promoter	1811	1816	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K2055019	.	operator	1817	1833	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K2055019	.	engineered_region	1792	1845	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K2055019	.	operator	1794	1810	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
>BBa_K2055019
actcgtacccataacgttgcatgccagagtccaggcgtacagtcgcttggctggcgtcctattgatgcccgtgaaggag
gtgtaaaatgtactagagaaagaggagaaatactagatgattgatgagaggatgaactcggacgaactgaaacgggctg
gtctcaaggccaccctcccacgactcaagatcctacggatattcgaagattcggatgctcgacatctgaccgcaggtga
gatctatcggctcttgctggaaaccggcgaagaagtgggcttggcgaccgtatatcgtgtactgacgcagttcgagatg
gcaggtttggtccgccgccatcattttgagggcgataaggccgtgttcgagctgaacgagaccggtcaccacgaccaca
tggtgtgtactgcgtgtggaaaggtgctcgagtttttcgacgaaatgctggaagcccggcaacgcgagctcgccgccaa
tcgcggtttcttcatttcggatcatagtctctatctttatggcacctgtctgggcatgcaggacgtgggaatctgctcg
cttcgcgacgacgacgcgccaggtgccagtactgactgataatactagagtgtaataagactcattcgttactagagaa
agaggagaaatactagatgaaacccgtaacgctctacgatgtggcggaatacgctggcgtgtcttaccagaccgtcagc
agggttgtgaatcaagcctcccatgtatcggcgaaaacccgtgagaaagtggaggcggcaatggctgaactgaactata
tcccaaaccgagttgctcaacagctcgcaggcaaacagagtctcctcattggcgtcgccacgtcctcgttggctctgca
tgccccgagccagattgtggcggcgatcaagtcccgcgccgaccagcttggagcgtcggtggttgtctctatggtggaa
cggtcaggcgttgaggcgtgcaaggctgcggtacataacctgctggcccagcgagtaagcggcctcattattaactacc
ctctggacgatcaggacgctatcgctgtcgaggccgcatgtacaaacgtccccgccctgttcctcgacgtctcggatca
gacgcccatcaacagtataattttttctcatgaagacggcacccggttaggcgttgagcatctggtagccttgggccac
cagcagattgccctcctggcaggccctctttcgagtgtaagcgcccgcttgcgccttgcaggctggcataagtatctta
cccgaaaccagatccagccgatcgcggaacgcgagggagattggtccgccatgtcaggatttcaacagacgatgcaaat
gctcaacgaaggtatcgtaccgacggccatgttggtcgcgaacgaccaaatggccctgggcgcgatgcgggcgataacg
gaaagcggcctacgcgtcggagccgacatctcagtggtcggctacgacgatacggaagactcctcctgctatattcccc
ccctcacgaccattaagcaggactttcgcctgttgggacaaacgtccgttgatcgactccttcagctgtcgcagggtca
agccgtgaaaggaaatcagctcctgcctgtcagtctggtgaaacgcaagaccaccttggcccccaatacccaaaccgcc
agcccccgcgcgcttgcggattctctcatgcagttggctagacaggttagtcgcctggaatcgggacagtgataatact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacat
tgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatc
aaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaag
gtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcct
gtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttc
ccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgc
aagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaac
catgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaa
ctgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgctt
acaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaagg
tcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata