BBa_K2055019
type: ComponentDefinition
persistent identity: BBa K2055019
display id: BBa_K2055019
version: 1
was generated by: iGEM to SBOL conversion
description: Part designed for verification and characterization of the heat the shock promoter and Inverter.
created: 2016-09-27T11:00:00Z
modified: 2016-09-28T06:02:07Z
mutable provenance: Composite part
owned by: james
owned by: myers
mutable description: We used an existing RBS + RFP from iGEM parts. LacI and FUR proteins are optimized for Acidithiobacillus ferrooxidans. It has two RBS after the constitutive promoter and before the FUR CDS.
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30725
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: RFP is used as a reporter.
star: false
creator: Raul Garza & Carlos Vasquez
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K2055009
component: BBa_B0034
component: BBa_K2055010
component: BBa_B0012
component: BBa_K2055007
component: BBa_R0011
component: BBa_B0010
component: BBa_E1010
component: BBa_K2055013
sequence annotation: BBa_K2055013
sequence annotation: BBa_B0034
sequence annotation: BBa_K2055009
sequence annotation: BBa_K2055010
sequence annotation: BBa_K2055007
sequence annotation: BBa_B0012
sequence annotation: BBa_R0011
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K2055019_sequence/1

BBa_K2055009
type: Component
persistent identity: component2485336
display id: component2485336
version: 1
top level: BBa_K2055019
definition: BBa_K2055009
access: public

BBa_B0034
type: Component
persistent identity: component2485335
display id: component2485335
version: 1
top level: BBa_K2055019
definition: BBa_B0034
access: public

BBa_B0034
type: Component
persistent identity: component2485339
display id: component2485339
version: 1
top level: BBa_K2055019
definition: BBa_B0034
access: public

BBa_K2055010
type: Component
persistent identity: component2485337
display id: component2485337
version: 1
top level: BBa_K2055019
definition: BBa_K2055010
access: public

BBa_B0012
type: Component
persistent identity: component2485341
display id: component2485341
version: 1
top level: BBa_K2055019
definition: BBa_B0012
access: public

BBa_K2055007
type: Component
persistent identity: component2485340
display id: component2485340
version: 1
top level: BBa_K2055019
definition: BBa_K2055007
access: public

BBa_B0034
type: Component
persistent identity: component2485351
display id: component2485351
version: 1
top level: BBa_K2055019
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component2485345
display id: component2485345
version: 1
top level: BBa_K2055019
definition: lacI+pL
access: public

BBa_B0010
type: Component
persistent identity: component2485355
display id: component2485355
version: 1
top level: BBa_K2055019
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component2485354
display id: component2485354
version: 1
top level: BBa_K2055019
definition: mRFP1
access: public

BBa_B0012
type: Component
persistent identity: component2485357
display id: component2485357
version: 1
top level: BBa_K2055019
definition: BBa_B0012
access: public

BBa_K2055013
type: Component
persistent identity: component2485333
display id: component2485333
version: 1
top level: BBa_K2055019
definition: BBa_K2055013
access: public

BBa_K2055013
type: SequenceAnnotation
persistent identity: annotation2485333
display id: annotation2485333
version: 1
top level: BBa_K2055019
location: https://synbiohub.org/public/igem/BBa_K2055019/annotation2485333/range2485333/1
component: BBa_K2055013

https://synbiohub.org/public/igem/BBa_K2055019/annotation2485333/range2485333/1
type: Range
persistent identity: range2485333
display id: range2485333
version: 1
top level: BBa_K2055019
direction: direction/reverse
start: 1
end: 89
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2485335
display id: annotation2485335
version: 1
top level: BBa_K2055019
location: https://synbiohub.org/public/igem/BBa_K2055019/annotation2485335/range2485335/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2055019/annotation2485335/range2485335/1
type: Range
persistent identity: range2485335
display id: range2485335
version: 1
top level: BBa_K2055019
direction: direction/reverse
start: 98
end: 109
orientation: inline

BBa_K2055009
type: SequenceAnnotation
persistent identity: annotation2485336
display id: annotation2485336
version: 1
top level: BBa_K2055019
location: https://synbiohub.org/public/igem/BBa_K2055019/annotation2485336/range2485336/1
component: BBa_K2055009

https://synbiohub.org/public/igem/BBa_K2055019/annotation2485336/range2485336/1
type: Range
persistent identity: range2485336
display id: range2485336
version: 1
top level: BBa_K2055019
direction: direction/reverse
start: 116
end: 595
orientation: inline

BBa_K2055010
type: SequenceAnnotation
persistent identity: annotation2485337
display id: annotation2485337
version: 1
top level: BBa_K2055019
location: https://synbiohub.org/public/igem/BBa_K2055019/annotation2485337/range2485337/1
component: BBa_K2055010

https://synbiohub.org/public/igem/BBa_K2055019/annotation2485337/range2485337/1
type: Range
persistent identity: range2485337
display id: range2485337
version: 1
top level: BBa_K2055019
direction: direction/reverse
start: 604
end: 622
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2485339
display id: annotation2485339
version: 1
top level: BBa_K2055019
location: https://synbiohub.org/public/igem/BBa_K2055019/annotation2485339/range2485339/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2055019/annotation2485339/range2485339/1
type: Range
persistent identity: range2485339
display id: range2485339
version: 1
top level: BBa_K2055019
direction: direction/reverse
start: 631
end: 642
orientation: inline

BBa_K2055007
type: SequenceAnnotation
persistent identity: annotation2485340
display id: annotation2485340
version: 1
top level: BBa_K2055019
location: https://synbiohub.org/public/igem/BBa_K2055019/annotation2485340/range2485340/1
component: BBa_K2055007

https://synbiohub.org/public/igem/BBa_K2055019/annotation2485340/range2485340/1
type: Range
persistent identity: range2485340
display id: range2485340
version: 1
top level: BBa_K2055019
direction: direction/reverse
start: 649
end: 1734
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2485341
display id: annotation2485341
version: 1
top level: BBa_K2055019
location: https://synbiohub.org/public/igem/BBa_K2055019/annotation2485341/range2485341/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K2055019/annotation2485341/range2485341/1
type: Range
persistent identity: range2485341
display id: range2485341
version: 1
top level: BBa_K2055019
direction: direction/forward
start: 1743
end: 1783
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2485345
display id: annotation2485345
version: 1
top level: BBa_K2055019
location: https://synbiohub.org/public/igem/BBa_K2055019/annotation2485345/range2485345/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K2055019/annotation2485345/range2485345/1
type: Range
persistent identity: range2485345
display id: range2485345
version: 1
top level: BBa_K2055019
direction: direction/forward
start: 1792
end: 1845
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2485351
display id: annotation2485351
version: 1
top level: BBa_K2055019
location: https://synbiohub.org/public/igem/BBa_K2055019/annotation2485351/range2485351/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2055019/annotation2485351/range2485351/1
type: Range
persistent identity: range2485351
display id: range2485351
version: 1
top level: BBa_K2055019
direction: direction/reverse
start: 1855
end: 1866
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2485354
display id: annotation2485354
version: 1
top level: BBa_K2055019
location: https://synbiohub.org/public/igem/BBa_K2055019/annotation2485354/range2485354/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K2055019/annotation2485354/range2485354/1
type: Range
persistent identity: range2485354
display id: range2485354
version: 1
top level: BBa_K2055019
direction: direction/reverse
start: 1873
end: 2578
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2485355
display id: annotation2485355
version: 1
top level: BBa_K2055019
location: https://synbiohub.org/public/igem/BBa_K2055019/annotation2485355/range2485355/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K2055019/annotation2485355/range2485355/1
type: Range
persistent identity: range2485355
display id: range2485355
version: 1
top level: BBa_K2055019
direction: direction/forward
start: 2587
end: 2666
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2485357
display id: annotation2485357
version: 1
top level: BBa_K2055019
location: https://synbiohub.org/public/igem/BBa_K2055019/annotation2485357/range2485357/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K2055019/annotation2485357/range2485357/1
type: Range
persistent identity: range2485357
display id: range2485357
version: 1
top level: BBa_K2055019
direction: direction/forward
start: 2675
end: 2715
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K2055007
type: ComponentDefinition
persistent identity: BBa K2055007
display id: BBa_K2055007
version: 1
was generated by: iGEM to SBOL conversion
description: LacI for Acidithiobacillus
created: 2016-09-27T11:00:00Z
modified: 2016-09-28T06:11:09Z
mutable provenance: The RBS used is the BBa_B0034, the terminator is the BBA_B0012, the LacI reculated Promoter is the BBa_R0011. The sequence for the FUR Box was obtained from the results of the paper of Quatrini et al. (Citation in our wiki), choosing the Fur box with the best score. The sequence for the FUR protein was obtained from the Acidithiobacillus ferrooxidans genome.
owned by: james
owned by: myers
mutable description: Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of iron. LacI and FUR proteins are optimized for Acidithiobacillus ferrooxidans.
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30725
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: It does not have a promoter.
star: false
creator: Carmen Padilla, Melissa Gonzalez & Melissa Rios
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2055007_sequence/1

BBa_K2055009
type: ComponentDefinition
persistent identity: BBa K2055009
display id: BBa_K2055009
version: 1
was generated by: iGEM to SBOL conversion
description: FUR Protein
created: 2016-09-27T11:00:00Z
modified: 2016-09-28T04:06:55Z
mutable provenance: The sequence for the FUR Box was obtained from the results of the paper of Quatrini et al. (Citation in our wiki).
owned by: james
owned by: myers
mutable description: Ferric Uptace Regulator Protein codon-optimized for Acidithiobacillus Ferrooxidans, the FUR Protein in presence of iron will block the transcription because it will bind to its FUR Box.
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30725
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: You will have to add a FUR Box to repress the transcription with this FUR protein in presence of iron.
star: false
creator: Carlos Vasquez
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2055009_sequence/1

BBa_K2055010
type: ComponentDefinition
persistent identity: BBa K2055010
display id: BBa_K2055010
version: 1
was generated by: iGEM to SBOL conversion
description: FUR Box
created: 2016-09-27T11:00:00Z
modified: 2016-09-28T04:07:16Z
mutable provenance: It is the FUR Box with the highest score in the work of: Quatrini, R., Lefimil, C., Veloso, F. A., Pedroso, I., Holmes, D. S., & Jedlicki, E. (2007). Bioinformatic prediction and experimental verification of Fur-regulated genes in the extreme acidophile Acidithiobacillus ferrooxidans. Nucleic Acids Research, 35(7), 2153???2166. http://doi.org/10.1093/nar/gkm068
owned by: james
owned by: myers
mutable description: This is a FUR Box with high affinity to BBa_K2055009. In presence of iron, it will block the downstream transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30725
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: It needs iron to block transcription.
star: false
creator: Carlos Vasquez
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K2055010_sequence/1

BBa_K2055013
type: ComponentDefinition
persistent identity: BBa K2055013
display id: BBa_K2055013
version: 1
was generated by: iGEM to SBOL conversion
description: Heat Shock Promoter
created: 2016-09-27T11:00:00Z
modified: 2016-09-28T04:19:49Z
mutable provenance: Ribeiro, D. A., Del Bem, L. E., Vicentini, R., Ferraz, L. F., Murakami, M. T., & Ottoboni, L. M. (2011). The small heat shock proteins from Acidithiobacillus ferrooxidans: gene expression, phylogenetic analysis, and structural modeling. BMC microbiology, 11(1), 1.
owned by: james
owned by: myers
mutable description: σ32-dependent promoter for for the Afe_1437 gene in Acidithiobacillus ferrooxidans
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30725
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Putative promoter in Acidithiobacillus ferrooxidans. Must check its characterization.
star: false
creator: Raul Garza
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K2055013_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

https://synbiohub.org/public/igem/BBa_K2055019_sequence/1
type: Sequence
persistent identity: BBa K2055019 sequence
display id: BBa_K2055019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actcgtacccataacgttgcatgccagagtccaggcgtacagtcgcttggctggcgtcctattgatgcccgtgaaggaggtgtaaaatgtactagagaaagaggagaaatactagatgattgatgagaggatgaactcggacgaactgaaacgggctggtctcaaggccaccctcccacgactcaagatcctacggatattcgaagattcggatgctcgacatctgaccgcaggtgagatctatcggctcttgctggaaaccggcgaagaagtgggcttggcgaccgtatatcgtgtactgacgcagttcgagatggcaggtttggtccgccgccatcattttgagggcgataaggccgtgttcgagctgaacgagaccggtcaccacgaccacatggtgtgtactgcgtgtggaaaggtgctcgagtttttcgacgaaatgctggaagcccggcaacgcgagctcgccgccaatcgcggtttcttcatttcggatcatagtctctatctttatggcacctgtctgggcatgcaggacgtgggaatctgctcgcttcgcgacgacgacgcgccaggtgccagtactgactgataatactagagtgtaataagactcattcgttactagagaaagaggagaaatactagatgaaacccgtaacgctctacgatgtggcggaatacgctggcgtgtcttaccagaccgtcagcagggttgtgaatcaagcctcccatgtatcggcgaaaacccgtgagaaagtggaggcggcaatggctgaactgaactatatcccaaaccgagttgctcaacagctcgcaggcaaacagagtctcctcattggcgtcgccacgtcctcgttggctctgcatgccccgagccagattgtggcggcgatcaagtcccgcgccgaccagcttggagcgtcggtggttgtctctatggtggaacggtcaggcgttgaggcgtgcaaggctgcggtacataacctgctggcccagcgagtaagcggcctcattattaactaccctctggacgatcaggacgctatcgctgtcgaggccgcatgtacaaacgtccccgccctgttcctcgacgtctcggatcagacgcccatcaacagtataattttttctcatgaagacggcacccggttaggcgttgagcatctggtagccttgggccaccagcagattgccctcctggcaggccctctttcgagtgtaagcgcccgcttgcgccttgcaggctggcataagtatcttacccgaaaccagatccagccgatcgcggaacgcgagggagattggtccgccatgtcaggatttcaacagacgatgcaaatgctcaacgaaggtatcgtaccgacggccatgttggtcgcgaacgaccaaatggccctgggcgcgatgcgggcgataacggaaagcggcctacgcgtcggagccgacatctcagtggtcggctacgacgatacggaagactcctcctgctatattccccccctcacgaccattaagcaggactttcgcctgttgggacaaacgtccgttgatcgactccttcagctgtcgcagggtcaagccgtgaaaggaaatcagctcctgcctgtcagtctggtgaaacgcaagaccaccttggcccccaatacccaaaccgccagcccccgcgcgcttgcggattctctcatgcagttggctagacaggttagtcgcctggaatcgggacagtgataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2055007_sequence/1
type: Sequence
persistent identity: BBa K2055007 sequence
display id: BBa_K2055007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaacccgtaacgctctacgatgtggcggaatacgctggcgtgtcttaccagaccgtcagcagggttgtgaatcaagcctcccatgtatcggcgaaaacccgtgagaaagtggaggcggcaatggctgaactgaactatatcccaaaccgagttgctcaacagctcgcaggcaaacagagtctcctcattggcgtcgccacgtcctcgttggctctgcatgccccgagccagattgtggcggcgatcaagtcccgcgccgaccagcttggagcgtcggtggttgtctctatggtggaacggtcaggcgttgaggcgtgcaaggctgcggtacataacctgctggcccagcgagtaagcggcctcattattaactaccctctggacgatcaggacgctatcgctgtcgaggccgcatgtacaaacgtccccgccctgttcctcgacgtctcggatcagacgcccatcaacagtataattttttctcatgaagacggcacccggttaggcgttgagcatctggtagccttgggccaccagcagattgccctcctggcaggccctctttcgagtgtaagcgcccgcttgcgccttgcaggctggcataagtatcttacccgaaaccagatccagccgatcgcggaacgcgagggagattggtccgccatgtcaggatttcaacagacgatgcaaatgctcaacgaaggtatcgtaccgacggccatgttggtcgcgaacgaccaaatggccctgggcgcgatgcgggcgataacggaaagcggcctacgcgtcggagccgacatctcagtggtcggctacgacgatacggaagactcctcctgctatattccccccctcacgaccattaagcaggactttcgcctgttgggacaaacgtccgttgatcgactccttcagctgtcgcagggtcaagccgtgaaaggaaatcagctcctgcctgtcagtctggtgaaacgcaagaccaccttggcccccaatacccaaaccgccagcccccgcgcgcttgcggattctctcatgcagttggctagacaggttagtcgcctggaatcgggacagtgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2055009_sequence/1
type: Sequence
persistent identity: BBa K2055009 sequence
display id: BBa_K2055009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgatgagaggatgaactcggacgaactgaaacgggctggtctcaaggccaccctcccacgactcaagatcctacggatattcgaagattcggatgctcgacatctgaccgcaggtgagatctatcggctcttgctggaaaccggcgaagaagtgggcttggcgaccgtatatcgtgtactgacgcagttcgagatggcaggtttggtccgccgccatcattttgagggcgataaggccgtgttcgagctgaacgagaccggtcaccacgaccacatggtgtgtactgcgtgtggaaaggtgctcgagtttttcgacgaaatgctggaagcccggcaacgcgagctcgccgccaatcgcggtttcttcatttcggatcatagtctctatctttatggcacctgtctgggcatgcaggacgtgggaatctgctcgcttcgcgacgacgacgcgccaggtgccagtactgactgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2055010_sequence/1
type: Sequence
persistent identity: BBa K2055010 sequence
display id: BBa_K2055010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtaataagactcattcgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2055013_sequence/1
type: Sequence
persistent identity: BBa K2055013 sequence
display id: BBa_K2055013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actcgtacccataacgttgcatgccagagtccaggcgtacagtcgcttggctggcgtcctattgatgcccgtgaaggaggtgtaaaatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z