LOCUS BBa_K2055020 3478 bp DNA linear 26-Nov-2024 DEFINITION Overexpression of TetH with heat shock promoter and Inverter. ACCESSION BBa_K2055020 VERSION BBa_K2055020.1 FEATURES Location/Qualifiers promoter 1..89 /label=BBa_K2055013 misc_feature 102..105 /label=conserved RBS 98..109 /label=BBa_B0034 CDS 116..595 /label=BBa_K2055009 promoter 604..622 /label=BBa_K2055010 misc_feature 635..638 /label=conserved RBS 631..642 /label=BBa_B0034 CDS 649..1734 /label=BBa_K2055007 stem_loop 1750..1769 /label=T7 TE misc_feature 1776..1776 /label=stop polyA_site 1770..1783 /label=polya misc_feature 1743..1783 /label=BBa_B0012 terminator 1743..1783 /label=BBa_B0012 misc_feature 1794..1810 /label=lac O1 promoter 1811..1816 /label=-35 misc_feature 1817..1833 /label=lac O1 promoter 1834..1839 /label=-10 misc_feature 1792..1845 /label=BBa_R0011 promoter 1792..1845 /label=BBa_R0011 misc_feature 1859..1862 /label=conserved RBS 1855..1866 /label=BBa_B0034 CDS 1873..3375 /label=BBa_K2055012 stem_loop 3391..3410 /label=T7 TE misc_feature 3417..3417 /label=stop polyA_site 3411..3424 /label=polya misc_feature 3384..3424 /label=BBa_B0012 terminator 3384..3424 /label=BBa_B0012 stem_loop 3445..3467 /label=stem_loop misc_feature 3433..3478 /label=BBa_B0011 terminator 3433..3478 /label=BBa_B0011 terminator 3384..3478 /label=BBa_B0014 ORIGIN 1 actcgtaccc ataacgttgc atgccagagt ccaggcgtac agtcgcttgg ctggcgtcct 61 attgatgccc gtgaaggagg tgtaaaatgt actagagaaa gaggagaaat actagatgat 121 tgatgagagg atgaactcgg acgaactgaa acgggctggt ctcaaggcca ccctcccacg 181 actcaagatc ctacggatat tcgaagattc ggatgctcga catctgaccg caggtgagat 241 ctatcggctc ttgctggaaa ccggcgaaga agtgggcttg gcgaccgtat atcgtgtact 301 gacgcagttc gagatggcag gtttggtccg ccgccatcat tttgagggcg ataaggccgt 361 gttcgagctg aacgagaccg gtcaccacga ccacatggtg tgtactgcgt gtggaaaggt 421 gctcgagttt ttcgacgaaa tgctggaagc ccggcaacgc gagctcgccg ccaatcgcgg 481 tttcttcatt tcggatcata gtctctatct ttatggcacc tgtctgggca tgcaggacgt 541 gggaatctgc tcgcttcgcg acgacgacgc gccaggtgcc agtactgact gataatacta 601 gagtgtaata agactcattc gttactagag aaagaggaga aatactagat gaaacccgta 661 acgctctacg atgtggcgga atacgctggc gtgtcttacc agaccgtcag cagggttgtg 721 aatcaagcct cccatgtatc ggcgaaaacc cgtgagaaag tggaggcggc aatggctgaa 781 ctgaactata tcccaaaccg agttgctcaa cagctcgcag gcaaacagag tctcctcatt 841 ggcgtcgcca cgtcctcgtt ggctctgcat gccccgagcc agattgtggc ggcgatcaag 901 tcccgcgccg accagcttgg agcgtcggtg gttgtctcta tggtggaacg gtcaggcgtt 961 gaggcgtgca aggctgcggt acataacctg ctggcccagc gagtaagcgg cctcattatt 1021 aactaccctc tggacgatca ggacgctatc gctgtcgagg ccgcatgtac aaacgtcccc 1081 gccctgttcc tcgacgtctc ggatcagacg cccatcaaca gtataatttt ttctcatgaa 1141 gacggcaccc ggttaggcgt tgagcatctg gtagccttgg gccaccagca gattgccctc 1201 ctggcaggcc ctctttcgag tgtaagcgcc cgcttgcgcc ttgcaggctg gcataagtat 1261 cttacccgaa accagatcca gccgatcgcg gaacgcgagg gagattggtc cgccatgtca 1321 ggatttcaac agacgatgca aatgctcaac gaaggtatcg taccgacggc catgttggtc 1381 gcgaacgacc aaatggccct gggcgcgatg cgggcgataa cggaaagcgg cctacgcgtc 1441 ggagccgaca tctcagtggt cggctacgac gatacggaag actcctcctg ctatattccc 1501 cccctcacga ccattaagca ggactttcgc ctgttgggac aaacgtccgt tgatcgactc 1561 cttcagctgt cgcagggtca agccgtgaaa ggaaatcagc tcctgcctgt cagtctggtg 1621 aaacgcaaga ccaccttggc ccccaatacc caaaccgcca gcccccgcgc gcttgcggat 1681 tctctcatgc agttggctag acaggttagt cgcctggaat cgggacagtg ataatactag 1741 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag 1801 cggataacaa ttgacattgt gagcggataa caagatactg agcacatact agagaaagag 1861 gagaaatact agatgccttc gattgtccgg aaccacggac cccacaacaa aattctgctg 1921 agcgccctcc tgctggcact gtttgggtgg gtgcccctgg cgagtgcggc ggtcgccgtg 1981 cccatggatt ctacgggtcc gtaccgcacg gtttcccatc ctgagaacgc cccgtcgggc 2041 gtggatgctg gcgtagggcc aagtgaatgg acccatgcgt atgccaaccc ggctcacaac 2101 gcagctttcc ccgtcccgga cgacgcgccc gagtggattc gcaacggggt cagttggctg 2161 tttccggagg cgcgggcgtg gcccctggct aaccctcctt tcggatcgaa aacctatggt 2221 gctgcggaag ccagcgtgac ccagacgcag ttctatggaa acgccctcgg tccaagtgtc 2281 gtggatggag tggtctatgc tgaatccgac gatatgtttg cgtatgcagt caacgccaag 2341 acggggaagc tgatctggcg cgcctcaccc gtgggcaaca atttgatggg gaacccgctc 2401 gtgattggga acaccgtata cctgagtgcc gggagcgtgg cctttaactt tgctaacgtc 2461 ctgaggtatg cgcataaccc ctcggcaagc gcccgcggat taaacgtgtc cttcaatggc 2521 atttatgcgc tgaatcggtc gaatggcaaa ctcttgtggt attttgcgac gccgggtgag 2581 accatggcga cgccggctta cgacaataac accttgttta ttgcggatgg cgcaggcaac 2641 gcattcggca tcaatgcgac aacaggaaaa caggtctgga agacgcacgt gggcggcatg 2701 gacaatatga gttccgtaac cgcttaccga cacaacattt acttcgccat ggctattaag 2761 ccgtatttgt attgcttgaa cgagtccaac ggccacatcg tctggaaggg cacgatccct 2821 ggcgccagca atacgggcat cggagacgtc agccccgcgg ctgcggatgg cgtggttgta 2881 ctcgatgcga ctaccaagcc gcaagccaat aaaaaagcta tgttcagcaa cgtgatccgt 2941 gcatttgacg ccaaaaccgg tgcagtgctc tggacccgca atatgggttc gggcggcaaa 3001 atccccgcct ttaaaggtgg cgtgcccatg atccacaata acattgtata tgtgggtaat 3061 ccggtggcga gcacctacca ggcgtacgag ctgaaaacgg gaaagctgct gtggacctgg 3121 cacgtcccca ccaaggtagc cgcaggtgca ggccgttcgg ccccgacata ctacaagggc 3181 ctgctttaca tcacgaccgg ccagtatatc tttgttgtca atccggccac cggaaaagag 3241 ctccatcagc atcatatcgg tggtcaattc ggtatcgaaa gccctgtgat cgttggcgga 3301 acggtgtacc tcaccaactc atgggattgg ataatggcaa tccccctgaa aaccatttcg 3361 cacggctcgt gataatacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 3421 tatatactag agagagaata taaaaagcca gattattaat ccggcttttt tattattt //