BBa_K2055020
type: ComponentDefinition
persistent identity: BBa K2055020
display id: BBa_K2055020
version: 1
was generated by: iGEM to SBOL conversion
description: Overexpression of TetH with heat shock promoter and Inverter.
created: 2016-09-27T11:00:00Z
modified: 2016-09-28T06:23:20Z
mutable provenance: Composite part
owned by: james
owned by: myers
mutable description: If the characterization of HS promoter + Inverter + RFP works, we will use this construct for our project, to recover Copper, Nickel and Zinc with Acidithiobacillus Ferrooxidans.
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30725
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Not tested yet.
star: false
creator: Raul Garza & Carlos Vasquez
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_R0011
component: BBa_B0012
component: BBa_K2055007
component: BBa_K2055010
component: BBa_K2055009
component: BBa_K2055013
component: BBa_B0014
component: BBa_K2055012
sequence annotation: BBa_K2055013
sequence annotation: BBa_B0034
sequence annotation: BBa_K2055009
sequence annotation: BBa_K2055010
sequence annotation: BBa_K2055007
sequence annotation: BBa_B0012
sequence annotation: BBa_R0011
sequence annotation: BBa_K2055012
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K2055020_sequence/1

BBa_B0034
type: Component
persistent identity: component2485379
display id: component2485379
version: 1
top level: BBa_K2055020
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component2485373
display id: component2485373
version: 1
top level: BBa_K2055020
definition: lacI+pL
access: public

BBa_B0012
type: Component
persistent identity: component2485369
display id: component2485369
version: 1
top level: BBa_K2055020
definition: BBa_B0012
access: public

BBa_K2055007
type: Component
persistent identity: component2485368
display id: component2485368
version: 1
top level: BBa_K2055020
definition: BBa_K2055007
access: public

BBa_B0034
type: Component
persistent identity: component2485367
display id: component2485367
version: 1
top level: BBa_K2055020
definition: BBa_B0034
access: public

BBa_K2055010
type: Component
persistent identity: component2485365
display id: component2485365
version: 1
top level: BBa_K2055020
definition: BBa_K2055010
access: public

BBa_K2055009
type: Component
persistent identity: component2485364
display id: component2485364
version: 1
top level: BBa_K2055020
definition: BBa_K2055009
access: public

BBa_B0034
type: Component
persistent identity: component2485363
display id: component2485363
version: 1
top level: BBa_K2055020
definition: BBa_B0034
access: public

BBa_K2055013
type: Component
persistent identity: component2485361
display id: component2485361
version: 1
top level: BBa_K2055020
definition: BBa_K2055013
access: public

BBa_B0014
type: Component
persistent identity: component2485387
display id: component2485387
version: 1
top level: BBa_K2055020
definition: BBa_B0014
access: public

BBa_K2055012
type: Component
persistent identity: component2485380
display id: component2485380
version: 1
top level: BBa_K2055020
definition: BBa_K2055012
access: public

BBa_K2055013
type: SequenceAnnotation
persistent identity: annotation2485361
display id: annotation2485361
version: 1
top level: BBa_K2055020
location: https://synbiohub.org/public/igem/BBa_K2055020/annotation2485361/range2485361/1
component: BBa_K2055013

https://synbiohub.org/public/igem/BBa_K2055020/annotation2485361/range2485361/1
type: Range
persistent identity: range2485361
display id: range2485361
version: 1
top level: BBa_K2055020
direction: direction/reverse
start: 1
end: 89
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2485363
display id: annotation2485363
version: 1
top level: BBa_K2055020
location: https://synbiohub.org/public/igem/BBa_K2055020/annotation2485363/range2485363/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2055020/annotation2485363/range2485363/1
type: Range
persistent identity: range2485363
display id: range2485363
version: 1
top level: BBa_K2055020
direction: direction/reverse
start: 98
end: 109
orientation: inline

BBa_K2055009
type: SequenceAnnotation
persistent identity: annotation2485364
display id: annotation2485364
version: 1
top level: BBa_K2055020
location: https://synbiohub.org/public/igem/BBa_K2055020/annotation2485364/range2485364/1
component: BBa_K2055009

https://synbiohub.org/public/igem/BBa_K2055020/annotation2485364/range2485364/1
type: Range
persistent identity: range2485364
display id: range2485364
version: 1
top level: BBa_K2055020
direction: direction/reverse
start: 116
end: 595
orientation: inline

BBa_K2055010
type: SequenceAnnotation
persistent identity: annotation2485365
display id: annotation2485365
version: 1
top level: BBa_K2055020
location: https://synbiohub.org/public/igem/BBa_K2055020/annotation2485365/range2485365/1
component: BBa_K2055010

https://synbiohub.org/public/igem/BBa_K2055020/annotation2485365/range2485365/1
type: Range
persistent identity: range2485365
display id: range2485365
version: 1
top level: BBa_K2055020
direction: direction/reverse
start: 604
end: 622
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2485367
display id: annotation2485367
version: 1
top level: BBa_K2055020
location: https://synbiohub.org/public/igem/BBa_K2055020/annotation2485367/range2485367/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2055020/annotation2485367/range2485367/1
type: Range
persistent identity: range2485367
display id: range2485367
version: 1
top level: BBa_K2055020
direction: direction/reverse
start: 631
end: 642
orientation: inline

BBa_K2055007
type: SequenceAnnotation
persistent identity: annotation2485368
display id: annotation2485368
version: 1
top level: BBa_K2055020
location: https://synbiohub.org/public/igem/BBa_K2055020/annotation2485368/range2485368/1
component: BBa_K2055007

https://synbiohub.org/public/igem/BBa_K2055020/annotation2485368/range2485368/1
type: Range
persistent identity: range2485368
display id: range2485368
version: 1
top level: BBa_K2055020
direction: direction/reverse
start: 649
end: 1734
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2485369
display id: annotation2485369
version: 1
top level: BBa_K2055020
location: https://synbiohub.org/public/igem/BBa_K2055020/annotation2485369/range2485369/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K2055020/annotation2485369/range2485369/1
type: Range
persistent identity: range2485369
display id: range2485369
version: 1
top level: BBa_K2055020
direction: direction/forward
start: 1743
end: 1783
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2485373
display id: annotation2485373
version: 1
top level: BBa_K2055020
location: https://synbiohub.org/public/igem/BBa_K2055020/annotation2485373/range2485373/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K2055020/annotation2485373/range2485373/1
type: Range
persistent identity: range2485373
display id: range2485373
version: 1
top level: BBa_K2055020
direction: direction/forward
start: 1792
end: 1845
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2485379
display id: annotation2485379
version: 1
top level: BBa_K2055020
location: https://synbiohub.org/public/igem/BBa_K2055020/annotation2485379/range2485379/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2055020/annotation2485379/range2485379/1
type: Range
persistent identity: range2485379
display id: range2485379
version: 1
top level: BBa_K2055020
direction: direction/reverse
start: 1855
end: 1866
orientation: inline

BBa_K2055012
type: SequenceAnnotation
persistent identity: annotation2485380
display id: annotation2485380
version: 1
top level: BBa_K2055020
location: https://synbiohub.org/public/igem/BBa_K2055020/annotation2485380/range2485380/1
component: BBa_K2055012

https://synbiohub.org/public/igem/BBa_K2055020/annotation2485380/range2485380/1
type: Range
persistent identity: range2485380
display id: range2485380
version: 1
top level: BBa_K2055020
direction: direction/reverse
start: 1873
end: 3375
orientation: inline

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2485387
display id: annotation2485387
version: 1
top level: BBa_K2055020
location: https://synbiohub.org/public/igem/BBa_K2055020/annotation2485387/range2485387/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K2055020/annotation2485387/range2485387/1
type: Range
persistent identity: range2485387
display id: range2485387
version: 1
top level: BBa_K2055020
direction: direction/reverse
start: 3384
end: 3478
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K2055007
type: ComponentDefinition
persistent identity: BBa K2055007
display id: BBa_K2055007
version: 1
was generated by: iGEM to SBOL conversion
description: LacI for Acidithiobacillus
created: 2016-09-27T11:00:00Z
modified: 2016-09-28T06:11:09Z
mutable provenance: The RBS used is the BBa_B0034, the terminator is the BBA_B0012, the LacI reculated Promoter is the BBa_R0011. The sequence for the FUR Box was obtained from the results of the paper of Quatrini et al. (Citation in our wiki), choosing the Fur box with the best score. The sequence for the FUR protein was obtained from the Acidithiobacillus ferrooxidans genome.
owned by: james
owned by: myers
mutable description: Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of iron. LacI and FUR proteins are optimized for Acidithiobacillus ferrooxidans.
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30725
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: It does not have a promoter.
star: false
creator: Carmen Padilla, Melissa Gonzalez & Melissa Rios
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2055007_sequence/1

BBa_K2055009
type: ComponentDefinition
persistent identity: BBa K2055009
display id: BBa_K2055009
version: 1
was generated by: iGEM to SBOL conversion
description: FUR Protein
created: 2016-09-27T11:00:00Z
modified: 2016-09-28T04:06:55Z
mutable provenance: The sequence for the FUR Box was obtained from the results of the paper of Quatrini et al. (Citation in our wiki).
owned by: james
owned by: myers
mutable description: Ferric Uptace Regulator Protein codon-optimized for Acidithiobacillus Ferrooxidans, the FUR Protein in presence of iron will block the transcription because it will bind to its FUR Box.
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30725
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: You will have to add a FUR Box to repress the transcription with this FUR protein in presence of iron.
star: false
creator: Carlos Vasquez
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2055009_sequence/1

BBa_K2055010
type: ComponentDefinition
persistent identity: BBa K2055010
display id: BBa_K2055010
version: 1
was generated by: iGEM to SBOL conversion
description: FUR Box
created: 2016-09-27T11:00:00Z
modified: 2016-09-28T04:07:16Z
mutable provenance: It is the FUR Box with the highest score in the work of: Quatrini, R., Lefimil, C., Veloso, F. A., Pedroso, I., Holmes, D. S., & Jedlicki, E. (2007). Bioinformatic prediction and experimental verification of Fur-regulated genes in the extreme acidophile Acidithiobacillus ferrooxidans. Nucleic Acids Research, 35(7), 2153???2166. http://doi.org/10.1093/nar/gkm068
owned by: james
owned by: myers
mutable description: This is a FUR Box with high affinity to BBa_K2055009. In presence of iron, it will block the downstream transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30725
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: It needs iron to block transcription.
star: false
creator: Carlos Vasquez
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K2055010_sequence/1

BBa_K2055012
type: ComponentDefinition
persistent identity: BBa K2055012
display id: BBa_K2055012
version: 1
was generated by: iGEM to SBOL conversion
description: Tetrathionate Hydrolase
created: 2016-09-27T11:00:00Z
modified: 2016-09-28T04:14:05Z
mutable provenance: It comes from the Acidithiobacillus Ferrooxidans genome.
owned by: james
owned by: myers
mutable description: Tetrathionate Hydrolase, TetH codon-optimized for Acidithiobacillus Ferrooxidans. It codes for the enzyme that converts tetrathionate to thiosulfate and sulfur.
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30725
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: It is an extracellular matrix enzyme for A. Ferrooxidans.
star: false
creator: Carlos Vasquez
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2055012_sequence/1

BBa_K2055013
type: ComponentDefinition
persistent identity: BBa K2055013
display id: BBa_K2055013
version: 1
was generated by: iGEM to SBOL conversion
description: Heat Shock Promoter
created: 2016-09-27T11:00:00Z
modified: 2016-09-28T04:19:49Z
mutable provenance: Ribeiro, D. A., Del Bem, L. E., Vicentini, R., Ferraz, L. F., Murakami, M. T., & Ottoboni, L. M. (2011). The small heat shock proteins from Acidithiobacillus ferrooxidans: gene expression, phylogenetic analysis, and structural modeling. BMC microbiology, 11(1), 1.
owned by: james
owned by: myers
mutable description: σ32-dependent promoter for for the Afe_1437 gene in Acidithiobacillus ferrooxidans
discontinued: false
dominant: false
experience: experience/None
group u list: _2523_
m user id: 30725
owner id: 30725
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Putative promoter in Acidithiobacillus ferrooxidans. Must check its characterization.
star: false
creator: Raul Garza
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K2055013_sequence/1

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

https://synbiohub.org/public/igem/BBa_K2055020_sequence/1
type: Sequence
persistent identity: BBa K2055020 sequence
display id: BBa_K2055020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actcgtacccataacgttgcatgccagagtccaggcgtacagtcgcttggctggcgtcctattgatgcccgtgaaggaggtgtaaaatgtactagagaaagaggagaaatactagatgattgatgagaggatgaactcggacgaactgaaacgggctggtctcaaggccaccctcccacgactcaagatcctacggatattcgaagattcggatgctcgacatctgaccgcaggtgagatctatcggctcttgctggaaaccggcgaagaagtgggcttggcgaccgtatatcgtgtactgacgcagttcgagatggcaggtttggtccgccgccatcattttgagggcgataaggccgtgttcgagctgaacgagaccggtcaccacgaccacatggtgtgtactgcgtgtggaaaggtgctcgagtttttcgacgaaatgctggaagcccggcaacgcgagctcgccgccaatcgcggtttcttcatttcggatcatagtctctatctttatggcacctgtctgggcatgcaggacgtgggaatctgctcgcttcgcgacgacgacgcgccaggtgccagtactgactgataatactagagtgtaataagactcattcgttactagagaaagaggagaaatactagatgaaacccgtaacgctctacgatgtggcggaatacgctggcgtgtcttaccagaccgtcagcagggttgtgaatcaagcctcccatgtatcggcgaaaacccgtgagaaagtggaggcggcaatggctgaactgaactatatcccaaaccgagttgctcaacagctcgcaggcaaacagagtctcctcattggcgtcgccacgtcctcgttggctctgcatgccccgagccagattgtggcggcgatcaagtcccgcgccgaccagcttggagcgtcggtggttgtctctatggtggaacggtcaggcgttgaggcgtgcaaggctgcggtacataacctgctggcccagcgagtaagcggcctcattattaactaccctctggacgatcaggacgctatcgctgtcgaggccgcatgtacaaacgtccccgccctgttcctcgacgtctcggatcagacgcccatcaacagtataattttttctcatgaagacggcacccggttaggcgttgagcatctggtagccttgggccaccagcagattgccctcctggcaggccctctttcgagtgtaagcgcccgcttgcgccttgcaggctggcataagtatcttacccgaaaccagatccagccgatcgcggaacgcgagggagattggtccgccatgtcaggatttcaacagacgatgcaaatgctcaacgaaggtatcgtaccgacggccatgttggtcgcgaacgaccaaatggccctgggcgcgatgcgggcgataacggaaagcggcctacgcgtcggagccgacatctcagtggtcggctacgacgatacggaagactcctcctgctatattccccccctcacgaccattaagcaggactttcgcctgttgggacaaacgtccgttgatcgactccttcagctgtcgcagggtcaagccgtgaaaggaaatcagctcctgcctgtcagtctggtgaaacgcaagaccaccttggcccccaatacccaaaccgccagcccccgcgcgcttgcggattctctcatgcagttggctagacaggttagtcgcctggaatcgggacagtgataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgccttcgattgtccggaaccacggaccccacaacaaaattctgctgagcgccctcctgctggcactgtttgggtgggtgcccctggcgagtgcggcggtcgccgtgcccatggattctacgggtcc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2055007_sequence/1
type: Sequence
persistent identity: BBa K2055007 sequence
display id: BBa_K2055007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaacccgtaacgctctacgatgtggcggaatacgctggcgtgtcttaccagaccgtcagcagggttgtgaatcaagcctcccatgtatcggcgaaaacccgtgagaaagtggaggcggcaatggctgaactgaactatatcccaaaccgagttgctcaacagctcgcaggcaaacagagtctcctcattggcgtcgccacgtcctcgttggctctgcatgccccgagccagattgtggcggcgatcaagtcccgcgccgaccagcttggagcgtcggtggttgtctctatggtggaacggtcaggcgttgaggcgtgcaaggctgcggtacataacctgctggcccagcgagtaagcggcctcattattaactaccctctggacgatcaggacgctatcgctgtcgaggccgcatgtacaaacgtccccgccctgttcctcgacgtctcggatcagacgcccatcaacagtataattttttctcatgaagacggcacccggttaggcgttgagcatctggtagccttgggccaccagcagattgccctcctggcaggccctctttcgagtgtaagcgcccgcttgcgccttgcaggctggcataagtatcttacccgaaaccagatccagccgatcgcggaacgcgagggagattggtccgccatgtcaggatttcaacagacgatgcaaatgctcaacgaaggtatcgtaccgacggccatgttggtcgcgaacgaccaaatggccctgggcgcgatgcgggcgataacggaaagcggcctacgcgtcggagccgacatctcagtggtcggctacgacgatacggaagactcctcctgctatattccccccctcacgaccattaagcaggactttcgcctgttgggacaaacgtccgttgatcgactccttcagctgtcgcagggtcaagccgtgaaaggaaatcagctcctgcctgtcagtctggtgaaacgcaagaccaccttggcccccaatacccaaaccgccagcccccgcgcgcttgcggattctctcatgcagttggctagacaggttagtcgcctggaatcgggacagtgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2055009_sequence/1
type: Sequence
persistent identity: BBa K2055009 sequence
display id: BBa_K2055009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgatgagaggatgaactcggacgaactgaaacgggctggtctcaaggccaccctcccacgactcaagatcctacggatattcgaagattcggatgctcgacatctgaccgcaggtgagatctatcggctcttgctggaaaccggcgaagaagtgggcttggcgaccgtatatcgtgtactgacgcagttcgagatggcaggtttggtccgccgccatcattttgagggcgataaggccgtgttcgagctgaacgagaccggtcaccacgaccacatggtgtgtactgcgtgtggaaaggtgctcgagtttttcgacgaaatgctggaagcccggcaacgcgagctcgccgccaatcgcggtttcttcatttcggatcatagtctctatctttatggcacctgtctgggcatgcaggacgtgggaatctgctcgcttcgcgacgacgacgcgccaggtgccagtactgactgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2055010_sequence/1
type: Sequence
persistent identity: BBa K2055010 sequence
display id: BBa_K2055010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtaataagactcattcgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2055012_sequence/1
type: Sequence
persistent identity: BBa K2055012 sequence
display id: BBa_K2055012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgccttcgattgtccggaaccacggaccccacaacaaaattctgctgagcgccctcctgctggcactgtttgggtgggtgcccctggcgagtgcggcggtcgccgtgcccatggattctacgggtccgtaccgcacggtttcccatcctgagaacgccccgtcgggcgtggatgctggcgtagggccaagtgaatggacccatgcgtatgccaacccggctcacaacgcagctttccccgtcccggacgacgcgcccgagtggattcgcaacggggtcagttggctgtttccggaggcgcgggcgtggcccctggctaaccctcctttcggatcgaaaacctatggtgctgcggaagccagcgtgacccagacgcagttctatggaaacgccctcggtccaagtgtcgtggatggagtggtctatgctgaatccgacgatatgtttgcgtatgcagtcaacgccaagacggggaagctgatctggcgcgcctcacccgtgggcaacaatttgatggggaacccgctcgtgattgggaacaccgtatacctgagtgccgggagcgtggcctttaactttgctaacgtcctgaggtatgcgcataacccctcggcaagcgcccgcggattaaacgtgtccttcaatggcatttatgcgctgaatcggtcgaatggcaaactcttgtggtattttgcgacgccgggtgagaccatggcgacgccggcttacgacaataacaccttgtttattgcggatggcgcaggcaacgcattcggcatcaatgcgacaacaggaaaacaggtctggaagacgcacgtgggcggcatggacaatatgagttccgtaaccgcttaccgacacaacatttacttcgccatggctattaagccgtatttgtattgcttgaacgagtccaacggccacatcgtctggaagggcacgatccctggcgccagcaatacgggcatcggagacgtcagccccgcggctgcggatggcgtggttgtactcgatgcgactaccaagccgcaagccaataaaaaagctatgttcagcaacgtgatccgtgcatttgacgccaaaaccggtgcagtgctctggacccgcaatatgggttcgggcggcaaaatccccgcctttaaaggtggcgtgcccatgatccacaataacattgtatatgtgggtaatccggtggcgagcacctaccaggcgtacgagctgaaaacgggaaagctgctgtggacctggcacgtccccaccaaggtagccgcaggtgcaggccgttcggccccgacatactacaagggcctgctttacatcacgaccggccagtatatctttgttgtcaatccggccaccggaaaagagctccatcagcatcatatcggtggtcaattcggtatcgaaagccctgtgatcgttggcggaacggtgtacctcaccaactcatgggattggataatggcaatccccctgaaaaccatttcgcacggctcgtgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2055013_sequence/1
type: Sequence
persistent identity: BBa K2055013 sequence
display id: BBa_K2055013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: actcgtacccataacgttgcatgccagagtccaggcgtacagtcgcttggctggcgtcctattgatgcccgtgaaggaggtgtaaaatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z