BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939311
1
BBa_B0011
component939303
1
BBa_B0012
annotation939303
1
BBa_B0012
range939303
1
1
41
annotation939311
1
BBa_B0011
range939311
1
50
95
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_J61100
1
BBa_J61100
Ribosome Binding Site Family Member
2007-01-28T12:00:00Z
2015-08-31T02:03:00Z
N/A
{{JCA_Arkin_RBSFamily}}
false
true
_95_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_I712074
1
BBa_I712074
T7 promoter (strong promoter from T7 bacteriophage)
2007-10-21T11:00:00Z
2015-08-31T04:07:46Z
T7 bacteriophage
T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
false
false
_130_
0
1856
9
In stock
false
true
Rok Gaber
BBa_K2055025
1
BBa_K2055025
Sulfur reductase generator
2016-09-28T11:00:00Z
2016-09-29T04:10:13Z
The sequence of the sulfur reductase was obtained from UniProt. It corresponds to the SOR gene of Acidianus ambivalens, accession number P29082.
The UniProt Consortium. UniProt: a hub for protein information. Nucleic Acids Res. 43: D204-D212 (2015)
This composite part uses the T7 promoter in order to express the enzyme sulfur reductase, which catalyzes the simultaneous oxidation and reduction of elemental sulfur in the presence of oxygen, with sulfite and hydrogen sulfide as products.
false
false
_2523_
11825
11825
9
false
It should be used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase (DE3 systems).
false
Martha Carolina Elizondo Cant??
component2485402
1
BBa_J61100
component2485410
1
BBa_B0014
component2485401
1
BBa_I712074
component2485403
1
BBa_K2055002
annotation2485402
1
BBa_J61100
range2485402
1
55
66
annotation2485410
1
BBa_B0014
range2485410
1
1014
1108
annotation2485403
1
BBa_K2055002
range2485403
1
73
1005
annotation2485401
1
BBa_I712074
range2485401
1
1
46
BBa_K2055002
1
BBa_K2055002
Sulfur reductase
2016-09-28T11:00:00Z
2016-09-29T04:01:33Z
The sequence of the sulfur reductase was obtained from UniProt. It corresponds to the SOR gene of Acidianus ambivalens, accession number P29082.
Codes for the enzyme sulfur reductase, which catalyzes the simultaneous oxidation and reduction of elemental sulfur in the presence of oxygen, with sulfite and hydrogen sulfide as products.
false
false
_2523_
11825
11825
9
false
It was optimised for Escherichia coli.
false
Carolina Elizondo
BBa_K2055002_sequence
1
atgccaaagccgtatgtcgccataaatatggccgaattaaagaatgaacctaaaactttcgaaatgtttgcatccgtagggcctaaagtctgcatggtcactgcccgtcaccccggtttcgtaggtttccaaaaccacatacagataggcatactgcctttcgggaatcgctacggcggagcgaagatggacatgacaaaggagtcatcgaccgtccgcgtcttgcagtacacattttggaaggactggaaggaccacgaagagatgcatagacagaactggtcttacctttttcggttatgctatagttgcgcatctcaaatgatatggggtccctgggaaccaatatatgagataatatatgctaacatgcccattaacactgaaatgacggacttcacggctgtcgtgggaaagaaattcgccgagggaaaaccgttagatattccagtgatttcacaaccttatggaaaaagagtagtggcattcgctgagcactcggttatacccggcaaggagaagcagttcgaggatgcaatagtacggactcttgagatgctgaaaaaagcaccggggttcctgggtgcgatggttctgaaggaaatcggcgtgagcggaatcgggagtatgcagttcggtgcaaaggggttccaccaggtattagagaaccccggatcactggagcctgatcccaacaatgtcatgtatagcgtgccagaggccaaaaacacgccccagcaatacattgtgcatgttgagtgggcaaatactgacgcgcttatgttcggcatggggcgcgtccttttatacccagaactgcgccaggttcatgacgaagttcttgatacactggtgtacggtccctatatcagaatcttgaaccctatgatggaagggacattctggcgcgagtacttgaacgagcaataataa
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_J61100_sequence
1
aaagaggggaca
BBa_K2055025_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggggacatactagatgccaaagccgtatgtcgccataaatatggccgaattaaagaatgaacctaaaactttcgaaatgtttgcatccgtagggcctaaagtctgcatggtcactgcccgtcaccccggtttcgtaggtttccaaaaccacatacagataggcatactgcctttcgggaatcgctacggcggagcgaagatggacatgacaaaggagtcatcgaccgtccgcgtcttgcagtacacattttggaaggactggaaggaccacgaagagatgcatagacagaactggtcttacctttttcggttatgctatagttgcgcatctcaaatgatatggggtccctgggaaccaatatatgagataatatatgctaacatgcccattaacactgaaatgacggacttcacggctgtcgtgggaaagaaattcgccgagggaaaaccgttagatattccagtgatttcacaaccttatggaaaaagagtagtggcattcgctgagcactcggttatacccggcaaggagaagcagttcgaggatgcaatagtacggactcttgagatgctgaaaaaagcaccggggttcctgggtgcgatggttctgaaggaaatcggcgtgagcggaatcgggagtatgcagttcggtgcaaaggggttccaccaggtattagagaaccccggatcactggagcctgatcccaacaatgtcatgtatagcgtgccagaggccaaaaacacgccccagcaatacattgtgcatgttgagtgggcaaatactgacgcgcttatgttcggcatggggcgcgtccttttatacccagaactgcgccaggttcatgacgaagttcttgatacactggtgtacggtccctatatcagaatcttgaaccctatgatggaagggacattctggcgcgagtacttgaacgagcaataataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_I712074_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgca
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z