BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation7019
1
BBa_B0011
range7019
1
1
46
annotation1683
1
stem_loop
range1683
1
13
35
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939303
1
BBa_B0012
component939311
1
BBa_B0011
annotation939303
1
BBa_B0012
range939303
1
1
41
annotation939311
1
BBa_B0011
range939311
1
50
95
BBa_K2055004
1
BBa_K2055004
DNA coding for cyanide hydratase.
2016-10-11T11:00:00Z
2016-10-12T09:27:25Z
The cyanide hydratase gene sequence comes from A. niger's genome.
This construct codes for an enzyme called cyanide hydratase from A. niger, which catalyses the reaction from cyanide to formamide. This enzyme will be used once gold has been completely recovered from the media in order to convert cyanide into a less toxic compound for easier disposal.
false
false
_2523_
30722
30722
9
false
The coding sequence is optimized for its expression in C. violaceum.
false
Yili Natalia Padilla
BBa_K2055269
1
BBa_K2055269
rbs + cyanide hydratase + double terminator
2016-10-11T11:00:00Z
2016-10-12T09:37:28Z
cyanide hydratase [Aspergillus niger CBS 513.88]
NCBI Reference Sequence: XP_001389844.1
This construct codes for an enzyme called cyanide hydratase from A. niger, which catalyses the reaction from cyanide to formamide. This enzyme will be used once gold has been completely recovered from the media in order to convert cyanide into a less toxic compound for easier disposal.
false
false
_2523_
30722
30722
9
false
The coding sequence is optimized for its expression in C. violaceum.
false
Yili Natalia Padilla
component2495788
1
BBa_K2055004
component2495787
1
BBa_B0034
component2495795
1
BBa_B0014
annotation2495795
1
BBa_B0014
range2495795
1
1101
1195
annotation2495787
1
BBa_B0034
range2495787
1
1
12
annotation2495788
1
BBa_K2055004
range2495788
1
19
1092
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K2055269_sequence
1
aaagaggagaaatactagatggccccggtgctgaagaagtataaagccgcggccgtgaatgccgagccgggctggttcaacctggaggaatccgtgcggcgcaccatccactggattgacgaggccggcaaggcggggtgcaagttcatcgcgttccccgagctgtggatccctggatatccgtactggatgtggaaggtgaactaccaagagtccttgccgctgctgaaaaagtaccgggagaactccctgccgagcgatagcgatgaaatgcgccgcatccgaaacgcggcgcgcgccaacaagatctacgtctcgctgggctattcggaggttgatctggcctcgctgtacaccacccaggtgatgatctcgccaagcggcgacatcctgaaccatcggcggaagatccgggcaacgcacgtcgagaggctggttttcggcgacgggaccggcgatacgaccgagtcggtcatccagacggacatcggccgcgtgggccatctgaattgctgggaaaacatgaatcccttcatgaagagctacgccgcctctctgggagagcaagtgcatgtggccgcctggccgctgtatccgggaaaggagacgctgaagtacccggacccgttcaccaacgtggccgaggccaacgctgatctggtgaccccggcctacgcgatcgaaaccggcacctacactctggcgccgtggcagacaatcaccgccgaggggatcaagcttaacacccccccgggcaaggacctggaggaccctcacatctacaatggccatggccgcatcttcggacctgatggccaaaacctggtgccgcaccccgacaaggacttcgaaggcctgctgttcgtcgacatcgatctggacgagtgccacctgtccaagagcctggcggacttcggcggccactacatgcgcccggacctgattcgcctgctggtggacacgaatcgtaaagacctggtggtacgcgaagaccgggtgaacggcggcgtggaatacacccgcaccgtggaccgcgtgggtttgtccacccccctcgatatcgccaataccggcgaaagcgagaactaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K2055004_sequence
1
atggccccggtgctgaagaagtataaagccgcggccgtgaatgccgagccgggctggttcaacctggaggaatccgtgcggcgcaccatccactggattgacgaggccggcaaggcggggtgcaagttcatcgcgttccccgagctgtggatccctggatatccgtactggatgtggaaggtgaactaccaagagtccttgccgctgctgaaaaagtaccgggagaactccctgccgagcgatagcgatgaaatgcgccgcatccgaaacgcggcgcgcgccaacaagatctacgtctcgctgggctattcggaggttgatctggcctcgctgtacaccacccaggtgatgatctcgccaagcggcgacatcctgaaccatcggcggaagatccgggcaacgcacgtcgagaggctggttttcggcgacgggaccggcgatacgaccgagtcggtcatccagacggacatcggccgcgtgggccatctgaattgctgggaaaacatgaatcccttcatgaagagctacgccgcctctctgggagagcaagtgcatgtggccgcctggccgctgtatccgggaaaggagacgctgaagtacccggacccgttcaccaacgtggccgaggccaacgctgatctggtgaccccggcctacgcgatcgaaaccggcacctacactctggcgccgtggcagacaatcaccgccgaggggatcaagcttaacacccccccgggcaaggacctggaggaccctcacatctacaatggccatggccgcatcttcggacctgatggccaaaacctggtgccgcaccccgacaaggacttcgaaggcctgctgttcgtcgacatcgatctggacgagtgccacctgtccaagagcctggcggacttcggcggccactacatgcgcccggacctgattcgcctgctggtggacacgaatcgtaaagacctggtggtacgcgaagaccgggtgaacggcggcgtggaatacacccgcaccgtggaccgcgtgggtttgtccacccccctcgatatcgccaataccggcgaaagcgagaactaataa
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z