BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation1683 1 stem_loop range1683 1 13 35 annotation7019 1 BBa_B0011 range7019 1 1 46 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K2055004 1 BBa_K2055004 DNA coding for cyanide hydratase. 2016-10-11T11:00:00Z 2016-10-12T09:27:25Z The cyanide hydratase gene sequence comes from A. niger's genome. This construct codes for an enzyme called cyanide hydratase from A. niger, which catalyses the reaction from cyanide to formamide. This enzyme will be used once gold has been completely recovered from the media in order to convert cyanide into a less toxic compound for easier disposal. false false _2523_ 30722 30722 9 false The coding sequence is optimized for its expression in C. violaceum. false Yili Natalia Padilla BBa_K2055269 1 BBa_K2055269 rbs + cyanide hydratase + double terminator 2016-10-11T11:00:00Z 2016-10-12T09:37:28Z cyanide hydratase [Aspergillus niger CBS 513.88] NCBI Reference Sequence: XP_001389844.1 This construct codes for an enzyme called cyanide hydratase from A. niger, which catalyses the reaction from cyanide to formamide. This enzyme will be used once gold has been completely recovered from the media in order to convert cyanide into a less toxic compound for easier disposal. false false _2523_ 30722 30722 9 false The coding sequence is optimized for its expression in C. violaceum. false Yili Natalia Padilla component2495787 1 BBa_B0034 component2495795 1 BBa_B0014 component2495788 1 BBa_K2055004 annotation2495787 1 BBa_B0034 range2495787 1 1 12 annotation2495795 1 BBa_B0014 range2495795 1 1101 1195 annotation2495788 1 BBa_K2055004 range2495788 1 19 1092 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_B0014 1 BBa_B0014 double terminator (B0012-B0011) 2003-07-15T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0012 and BBa_B0011 false true _1_ 0 24 7 In stock false true Reshma Shetty component939303 1 BBa_B0012 component939311 1 BBa_B0011 annotation939311 1 BBa_B0011 range939311 1 50 95 annotation939303 1 BBa_B0012 range939303 1 1 41 BBa_K2055269_sequence 1 aaagaggagaaatactagatggccccggtgctgaagaagtataaagccgcggccgtgaatgccgagccgggctggttcaacctggaggaatccgtgcggcgcaccatccactggattgacgaggccggcaaggcggggtgcaagttcatcgcgttccccgagctgtggatccctggatatccgtactggatgtggaaggtgaactaccaagagtccttgccgctgctgaaaaagtaccgggagaactccctgccgagcgatagcgatgaaatgcgccgcatccgaaacgcggcgcgcgccaacaagatctacgtctcgctgggctattcggaggttgatctggcctcgctgtacaccacccaggtgatgatctcgccaagcggcgacatcctgaaccatcggcggaagatccgggcaacgcacgtcgagaggctggttttcggcgacgggaccggcgatacgaccgagtcggtcatccagacggacatcggccgcgtgggccatctgaattgctgggaaaacatgaatcccttcatgaagagctacgccgcctctctgggagagcaagtgcatgtggccgcctggccgctgtatccgggaaaggagacgctgaagtacccggacccgttcaccaacgtggccgaggccaacgctgatctggtgaccccggcctacgcgatcgaaaccggcacctacactctggcgccgtggcagacaatcaccgccgaggggatcaagcttaacacccccccgggcaaggacctggaggaccctcacatctacaatggccatggccgcatcttcggacctgatggccaaaacctggtgccgcaccccgacaaggacttcgaaggcctgctgttcgtcgacatcgatctggacgagtgccacctgtccaagagcctggcggacttcggcggccactacatgcgcccggacctgattcgcctgctggtggacacgaatcgtaaagacctggtggtacgcgaagaccgggtgaacggcggcgtggaatacacccgcaccgtggaccgcgtgggtttgtccacccccctcgatatcgccaataccggcgaaagcgagaactaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0014_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0034_sequence 1 aaagaggagaaa BBa_K2055004_sequence 1 atggccccggtgctgaagaagtataaagccgcggccgtgaatgccgagccgggctggttcaacctggaggaatccgtgcggcgcaccatccactggattgacgaggccggcaaggcggggtgcaagttcatcgcgttccccgagctgtggatccctggatatccgtactggatgtggaaggtgaactaccaagagtccttgccgctgctgaaaaagtaccgggagaactccctgccgagcgatagcgatgaaatgcgccgcatccgaaacgcggcgcgcgccaacaagatctacgtctcgctgggctattcggaggttgatctggcctcgctgtacaccacccaggtgatgatctcgccaagcggcgacatcctgaaccatcggcggaagatccgggcaacgcacgtcgagaggctggttttcggcgacgggaccggcgatacgaccgagtcggtcatccagacggacatcggccgcgtgggccatctgaattgctgggaaaacatgaatcccttcatgaagagctacgccgcctctctgggagagcaagtgcatgtggccgcctggccgctgtatccgggaaaggagacgctgaagtacccggacccgttcaccaacgtggccgaggccaacgctgatctggtgaccccggcctacgcgatcgaaaccggcacctacactctggcgccgtggcagacaatcaccgccgaggggatcaagcttaacacccccccgggcaaggacctggaggaccctcacatctacaatggccatggccgcatcttcggacctgatggccaaaacctggtgccgcaccccgacaaggacttcgaaggcctgctgttcgtcgacatcgatctggacgagtgccacctgtccaagagcctggcggacttcggcggccactacatgcgcccggacctgattcgcctgctggtggacacgaatcgtaaagacctggtggtacgcgaagaccgggtgaacggcggcgtggaatacacccgcaccgtggaccgcgtgggtttgtccacccccctcgatatcgccaataccggcgaaagcgagaactaataa BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z