BBa_K2055001
1
BBa_K2055001
Na(+)/H(+) antiporter NhaA
2016-09-28T11:00:00Z
2016-10-12T09:46:35Z
The sequence was obtained from Uniprot P13738. The origin for this sequence is E. Coli.
This construct codes for intermembrane protein Na(+)/H(+) antiporter NhaA, which is a proton pump from E. coli capable of excreting one Na(+) ion in exchange for two H(+) external protons. This protein is active at alkaline pH. This construct is regulated by a weak constitutive promoter and its objective is to augment bacterial resistance to alkaline pH.
false
false
_2523_
30722
30723
9
false
The coding sequence is optimized for its expression in C. violaceum and could be tested as a potential selective marker for C. violaceum. This construct is regulated by a weak constitutive promoter and its objective is to augment bacterial resistance to alkaline pH.
false
Suria Itzel Morales Guzm??n
BBa_B0014
1
BBa_B0014
double terminator (B0012-B0011)
2003-07-15T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0012 and BBa_B0011
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component939303
1
BBa_B0012
component939311
1
BBa_B0011
annotation939311
1
BBa_B0011
range939311
1
50
95
annotation939303
1
BBa_B0012
range939303
1
1
41
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation7019
1
BBa_B0011
range7019
1
1
46
annotation1683
1
stem_loop
range1683
1
13
35
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K2055369
1
BBa_K2055369
Na(+)/H(+) antiporter NhaA generator
2016-10-11T11:00:00Z
2016-10-12T09:58:23Z
The sequence was obtained from Uniprot P13738. The origin for this sequence is E. Coli.
This construct codes for intermembrane protein Na(+)/H(+) antiporter NhaA, which is a proton pump from E. coli capable of excreting one intracellular Na(+) ion in exchange for two H(+) external protons. This protein is active at alkaline pH. This construct is regulated by weak constitutive promoter and its objective is to augment bacterial resistance to alkaline pH.
false
false
_2523_
30722
30722
9
false
The coding sequence is optimized for its expression in C. violaceum and could be tested as a potential selective marker for C. violaceum. This construct is regulated by a weak constitutive promoter and its objective is to augment bacterial resistance to alkaline pH.
false
Suria Itzel Morales Guzm??n
component2495915
1
BBa_K2055001
component2495912
1
BBa_J23114
component2495922
1
BBa_B0014
component2495914
1
BBa_B0034
annotation2495912
1
BBa_J23114
range2495912
1
1
35
annotation2495915
1
BBa_K2055001
range2495915
1
62
1231
annotation2495922
1
BBa_B0014
range2495922
1
1240
1334
annotation2495914
1
BBa_B0034
range2495914
1
44
55
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_J23114
1
BBa_J23114
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_J23114_sequence
1
tttatggctagctcagtcctaggtacaatgctagc
BBa_B0014_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K2055369_sequence
1
tttatggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaaacatctgcatcgcttttttagcagcgatgcgagcggcggcattattctgattattgcggcgattctggcgatgattatggcgaacagcggcgcgaccagcggctggtatcatgattttctggaaaccccggtgcagctgcgcgtgggcagcctggaaattaacaaaaacatgctgctgtggattaacgatgcgctgatggcggtgttttttctgctggtgggcctggaagtgaaacgcgaactgatgcagggcagcctggcgagcctgcgccaggcggcgtttccggtgattgcggcgattggcggcatgattgtgccggcgctgctgtatctggcgtttaactatgcggatccgattacccgcgaaggctgggcgattccggcggcgaccgatattgcgtttgcgctgggcgtgctggcgctgctgggcagccgcgtgccgctggcgctgaaaatttttctgatggcgctggcgattattgatgatctgggcgcgattattattattgcgctgttttataccaacgatctgagcatggcgagcctgggcgtggcggcggtggcgattgcggtgctggcggtgctgaacctgtgcggcgcgcgccgcaccggcgtgtatattctggtgggcgtggtgctgtggaccgcggtgctgaaaagcggcgtgcatgcgaccctggcgggcgtgattgtgggcttttttattccgctgaaagaaaaacatggccgcagcccggcgaaacgcctggaacatgtgctgcatccgtgggtggcgtatctgattctgccgctgtttgcgtttgcgaacgcgggcgtgagcctgcaaggcgtgaccctggatggcctgaccagcattctgccgctgggcattattgcgggcctgctgattggcaaaccgctgggcattagcctgttttgctggctggcgctgcgcctgaaactggcgcatctgccggaaggcaccacctatcagcagattatggtggtgggcattctgtgcggcattggctttaccatgagcatttttattgcgagcctggcgtttggcagcgtggatccggaactgattaactgggcgaaactgggcattctggtgggcagcattagcagcgcggtgattggctatagctggctgcgcgtgcgcctgcgcccgagcgtgtaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_K2055001_sequence
1
atgaaacatctgcatcgcttttttagcagcgatgcgagcggcggcattattctgattattgcggcgattctggcgatgattatggcgaacagcggcgcgaccagcggctggtatcatgattttctggaaaccccggtgcagctgcgcgtgggcagcctggaaattaacaaaaacatgctgctgtggattaacgatgcgctgatggcggtgttttttctgctggtgggcctggaagtgaaacgcgaactgatgcagggcagcctggcgagcctgcgccaggcggcgtttccggtgattgcggcgattggcggcatgattgtgccggcgctgctgtatctggcgtttaactatgcggatccgattacccgcgaaggctgggcgattccggcggcgaccgatattgcgtttgcgctgggcgtgctggcgctgctgggcagccgcgtgccgctggcgctgaaaatttttctgatggcgctggcgattattgatgatctgggcgcgattattattattgcgctgttttataccaacgatctgagcatggcgagcctgggcgtggcggcggtggcgattgcggtgctggcggtgctgaacctgtgcggcgcgcgccgcaccggcgtgtatattctggtgggcgtggtgctgtggaccgcggtgctgaaaagcggcgtgcatgcgaccctggcgggcgtgattgtgggcttttttattccgctgaaagaaaaacatggccgcagcccggcgaaacgcctggaacatgtgctgcatccgtgggtggcgtatctgattctgccgctgtttgcgtttgcgaacgcgggcgtgagcctgcaaggcgtgaccctggatggcctgaccagcattctgccgctgggcattattgcgggcctgctgattggcaaaccgctgggcattagcctgttttgctggctggcgctgcgcctgaaactggcgcatctgccggaaggcaccacctatcagcagattatggtggtgggcattctgtgcggcattggctttaccatgagcatttttattgcgagcctggcgtttggcagcgtggatccggaactgattaactgggcgaaactgggcattctggtgggcagcattagcagcgcggtgattggctatagctggctgcgcgtgcgcctgcgcccgagcgtgtaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z