LOCUS       BBa_K2056003            1748 bp    DNA     linear       02-May-2024
DEFINITION  Carbon Monoxide sensor device (pCooF-Blue Chromoprotein)
ACCESSION   BBa_K2056003
VERSION     BBa_K2056003.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     misc_feature    65..67
                     /label=Start Codon
     CDS             65..733
                     /label=BBa_K352001
     stem_loop       753..796
                     /label=stem_loop
     misc_feature    742..821
                     /label=BBa_B0010
     terminator      742..821
                     /label=BBa_B0010
     misc_feature    830..899
                     /label=Spacer-1
     misc_feature    830..899
                     /label=BBa_B0040
     misc_feature    830..899
                     /label=BBa_B0040
     misc_feature    915..940
                     /label=A+T rich sequence
     misc_feature    948..951
                     /label=CooA binding site
     misc_feature    959..963
                     /label=CooA binding site
     misc_feature    986..991
                     /label=-10 Region
     misc_feature    999..1000
                     /label=Transcription Start
     misc_feature    943..968
                     /label=Protected by CooA
     misc_feature    930..998
                     /label=Protected by CooA+RNAP
     promoter        908..1001
                     /label=BBa_K352002
     RBS             1010..1024
                     /label=RBS-1\Strong
     RBS             1017..1021
                     /label=RBS
     misc_feature    1010..1024
                     /label=BBa_B0030
     RBS             1010..1024
                     /label=BBa_B0030
     CDS             1031..1696
                     /label=amilCP
     CDS             1031..1699
                     /label=BBa_K592009
     stem_loop       1715..1734
                     /label=T7 TE
     misc_feature    1741..1741
                     /label=stop
     polyA_site      1735..1748
                     /label=polya
     misc_feature    1708..1748
                     /label=BBa_B0012
     terminator      1708..1748
                     /label=BBa_B0012
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagattaaag aggagaaata
       61 ctagatgcca ccacgcttca atattgcaaa cgtactgctg tctcctgatg gtgagacgtt
      121 cttccgcggt tttcgctcta agattcatgc gaaaggctct ctggtatgta ctggtgaagg
      181 tgatgaaaac ggtgtttttg ttgtggttga tggtcgcctg cgtgtttacc tggttggtga
      241 ggagcgtgag attagcctgt tctacctgac ttccggcgat atgttctgca tgcattccgg
      301 ctgcctggtt gaagccaccg agcgcaccga agtgcgtttc gccgatatcc gcacgttcga
      361 gcagaaactg caaacctgtc cgtctatggc atggggcctg atcgccattc tgggccgtgc
      421 tctgacctcc tgtatgcgta ccatcgaaga cctgatgttc cacgatatta aacaacgtat
      481 cgcgggcttt ttcatcgacc acgctaacac taccggtcgc cagactcagg gtggcgtaat
      541 tgtttctgtt gacttcactg tagaggaaat cgctaatctg atcggtagct cccgccagac
      601 tactagcacg gcgctgaact ctctgattaa agagggttac atctcccgcc agggccgtgg
      661 tcactatact atcccgaacc tggttcgcct gaaggcggct gcggatggtg accgcgatga
      721 cgatgacgat tgatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      781 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagaga ggttctgtta
      841 agtaactgaa cccaatgtcg ttagtgacgc ttacctctta agaggtcact gacctaacat
      901 actagagttc ggcgtctttt cataccccca taaaaactct ggataactgt catctggccg
      961 acagacgggg ccgggctttt tgtcgcttac tcggcgccag gtactagaga ttaaagagga
     1021 gaaatactag atgagtgtga tcgctaaaca aatgacctac aaggtttata tgtcaggcac
     1081 ggtcaatgga cactactttg aggtcgaagg cgatggaaaa ggtaagccct acgaggggga
     1141 gcagacggta aagctcactg tcaccaaggg cggacctctg ccatttgctt gggatatttt
     1201 atcaccacag tgtcagtacg gaagcatacc attcaccaag taccctgaag acatccctga
     1261 ctatgtaaag cagtcattcc cggagggcta tacatgggag aggatcatga actttgaaga
     1321 tggtgcagtg tgtactgtca gcaatgattc cagcatccaa ggcaactgtt tcatctacca
     1381 tgtcaagttc tctggtttga actttcctcc caatggacct gtcatgcaga agaagacaca
     1441 gggctgggaa cccaacactg agcgtctctt tgcacgagat ggaatgctgc taggaaacaa
     1501 ctttatggct ctgaagttag aaggaggcgg tcactatttg tgtgaattta aaactactta
     1561 caaggcaaag aagcctgtga agatgccagg gtatcactat gttgaccgca aactggatgt
     1621 aaccaatcac aacaaggatt acacttcggt tgagcagtgt gaaatttcca ttgcacgcaa
     1681 acctgtggtc gcctaataat actagagtca cactggctca ccttcgggtg ggcctttctg
     1741 cgtttata
//