LOCUS       BBa_K2056004            1770 bp    DNA     linear       06-May-2024
DEFINITION  NOx(Oxides of Nitrogen) sensor device (pNirK-Yellow chromoprotein)
ACCESSION   BBa_K2056004
VERSION     BBa_K2056004.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     CDS             67..492
                     /label=BBa_K2056001
     stem_loop       512..555
                     /label=stem_loop
     misc_feature    501..580
                     /label=BBa_B0010
     terminator      501..580
                     /label=BBa_B0010
     misc_feature    589..658
                     /label=Spacer-1
     misc_feature    589..658
                     /label=BBa_B0040
     misc_feature    589..658
                     /label=BBa_B0040
     promoter        667..978
                     /label=BBa_K2056002
     RBS             987..1001
                     /label=RBS-1\Strong
     RBS             994..998
                     /label=RBS
     misc_feature    987..1001
                     /label=BBa_B0030
     RBS             987..1001
                     /label=BBa_B0030
     CDS             1008..1718
                     /label=fwYellow
     CDS             1008..1721
                     /label=BBa_K1033910
     stem_loop       1737..1756
                     /label=T7 TE
     misc_feature    1763..1763
                     /label=stop
     polyA_site      1757..1770
                     /label=polya
     misc_feature    1730..1770
                     /label=BBa_B0012
     terminator      1730..1770
                     /label=BBa_B0012
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagattaaag aggagaaata
       61 ctagaggtgc agttaacgag tttcactgat tacggattac gtgcgctgat ctacatggcg
      121 tcattgccag aagggcggat gaccagtatt tctgaagtga ctgacgtcta cggcgtctcc
      181 cgtaatcata tggtcaaaat aatcaatcaa cttagtcgtg ccggctacgt gactgctgtt
      241 cgtggaaaaa atggcggcat tcgcctgggt aaaccggcga gtgcgatacg tattggtgat
      301 gtggtgcgcg agctggagcc cttatcgctg gtgaattgca gcagtgagtt ttgccacatt
      361 acacctgcct gtaggttgaa acaggcactt tctaaggccg tgcaaagttt tcttacggaa
      421 ctggataact acacgcttgc cgatttggtt gaagagaatc aaccgcttta taaattattg
      481 ctggtggagt gatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
      541 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagag gttctgttaa
      601 gtaactgaac ccaatgtcgt tagtgacgct tacctcttaa gaggtcactg acctaacata
      661 ctagaggttg cgcgtaatac ggtcacgatt cgtaatttcc gtaacggagg acagtttctt
      721 tcatttcaga ctgagatttc cgaggatgct taacgcgtga ttgccgtgat tcgccagtcc
      781 catgaagtcc ttccaattta ttgagtgggc gttaataagt atattcctac attcttattt
      841 cttccagatt ggttaacgat ctaaattgaa cgcgtcaaaa gcatcaaacg ttatattaac
      901 aacattagtc tgcgtggcgg tgggtaatga tcgcctaaga agctgaacat gaccgtcttt
      961 aagggcttat cctgtagata ctagagatta aagaggagaa atactagatg acggcactga
     1021 ctgaaggcgc aaaactgttc gagaaagaaa tcccatatat cactgagctg gaaggtgacg
     1081 ttgaaggtat gaagtttatc atcaagggtg aaggtaccgg tgacgcgagc gtcggtaaag
     1141 tggatgctca gttcatttgt accacgggcg acgttccggt tccgtggagc acgctggtca
     1201 ccacgctgac gtatggtgct cagtgctttg ccaagtatcc gcgccacatt gcggatttct
     1261 tcaaaagctg catgccggaa ggttacgtcc aagagcgcac catcaccttt gagggtgatg
     1321 gcgtgttcaa gacccgtgcg gaagtcacct ttgaaaatgg cagcgtgtac aaccgtgtaa
     1381 aactgaacgg ccagggtttc aagaaggacg gccacgtgct gggcaaaaat ctggagttta
     1441 actttacccc tcattgtttg tacatttggg gtgaccaagc gaatcatggc ctgaagagcg
     1501 cgttcaaaat catgcatgag atcaccggct ccaaagagga tttcattgtt gccgatcaca
     1561 cccaaatgaa taccccgatt ggtggtggtc cggtgcacgt gccggagtac caccacatta
     1621 cgtatcatgt taccctgtct aaagacgtca ccgatcaccg tgaccatttg aacattgttg
     1681 aggtgatcaa ggcagttgac ctggagacgt accgttaata atactagagt cacactggct
     1741 caccttcggg tgggcctttc tgcgtttata
//