LOCUS BBa_K2056005 1558 bp DNA linear 22-Dec-2024 DEFINITION NOx(Oxides of Nitrogen) sensor device (pYeaR-Yellow chromoprotein) ACCESSION BBa_K2056005 VERSION BBa_K2056005.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23110 RBS 44..58 /label=RBS-1\Strong RBS 51..55 /label=RBS misc_feature 44..58 /label=BBa_B0030 RBS 44..58 /label=BBa_B0030 CDS 67..492 /label=BBa_K2056001 stem_loop 512..555 /label=stem_loop misc_feature 501..580 /label=BBa_B0010 terminator 501..580 /label=BBa_B0010 misc_feature 589..658 /label=Spacer-1 misc_feature 589..658 /label=BBa_B0040 misc_feature 589..658 /label=BBa_B0040 misc_feature 716..731 /label=NarL-binding region misc_feature 724..746 /label=NsrR-binding region misc_feature 732..737 /label=promoter -35 region misc_feature 755..760 /label=promoter -10 region promoter 667..766 /label=BBa_K216005 RBS 775..789 /label=RBS-1\Strong RBS 782..786 /label=RBS misc_feature 775..789 /label=BBa_B0030 RBS 775..789 /label=BBa_B0030 CDS 796..1506 /label=fwYellow CDS 796..1509 /label=BBa_K1033910 stem_loop 1525..1544 /label=T7 TE misc_feature 1551..1551 /label=stop polyA_site 1545..1558 /label=polya misc_feature 1518..1558 /label=BBa_B0012 terminator 1518..1558 /label=BBa_B0012 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagattaaag aggagaaata 61 ctagaggtgc agttaacgag tttcactgat tacggattac gtgcgctgat ctacatggcg 121 tcattgccag aagggcggat gaccagtatt tctgaagtga ctgacgtcta cggcgtctcc 181 cgtaatcata tggtcaaaat aatcaatcaa cttagtcgtg ccggctacgt gactgctgtt 241 cgtggaaaaa atggcggcat tcgcctgggt aaaccggcga gtgcgatacg tattggtgat 301 gtggtgcgcg agctggagcc cttatcgctg gtgaattgca gcagtgagtt ttgccacatt 361 acacctgcct gtaggttgaa acaggcactt tctaaggccg tgcaaagttt tcttacggaa 421 ctggataact acacgcttgc cgatttggtt gaagagaatc aaccgcttta taaattattg 481 ctggtggagt gatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 541 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagag gttctgttaa 601 gtaactgaac ccaatgtcgt tagtgacgct tacctcttaa gaggtcactg acctaacata 661 ctagagttcc catctataat cctccctgat tcttcgctga tatggtgcta aaaagtaacc 721 aataaatggt atttaaaatg caaattatca ggcgtaccct gaaacgtact agagattaaa 781 gaggagaaat actagatgac ggcactgact gaaggcgcaa aactgttcga gaaagaaatc 841 ccatatatca ctgagctgga aggtgacgtt gaaggtatga agtttatcat caagggtgaa 901 ggtaccggtg acgcgagcgt cggtaaagtg gatgctcagt tcatttgtac cacgggcgac 961 gttccggttc cgtggagcac gctggtcacc acgctgacgt atggtgctca gtgctttgcc 1021 aagtatccgc gccacattgc ggatttcttc aaaagctgca tgccggaagg ttacgtccaa 1081 gagcgcacca tcacctttga gggtgatggc gtgttcaaga cccgtgcgga agtcaccttt 1141 gaaaatggca gcgtgtacaa ccgtgtaaaa ctgaacggcc agggtttcaa gaaggacggc 1201 cacgtgctgg gcaaaaatct ggagtttaac tttacccctc attgtttgta catttggggt 1261 gaccaagcga atcatggcct gaagagcgcg ttcaaaatca tgcatgagat caccggctcc 1321 aaagaggatt tcattgttgc cgatcacacc caaatgaata ccccgattgg tggtggtccg 1381 gtgcacgtgc cggagtacca ccacattacg tatcatgtta ccctgtctaa agacgtcacc 1441 gatcaccgtg accatttgaa cattgttgag gtgatcaagg cagttgacct ggagacgtac 1501 cgttaataat actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //