BBa_K2056005
type: ComponentDefinition
persistent identity: BBa K2056005
display id: BBa_K2056005
version: 1
was generated by: iGEM to SBOL conversion
description: NOx(Oxides of Nitrogen) sensor device (pYeaR-Yellow chromoprotein)
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T01:38:25Z
mutable provenance: All parts in this sequence come from the Registry and have been listed. However, the complete part has been synthesized from IDT using their free gBLocks offer.
owned by: james
owned by: myers
mutable description: This part is the second oxides of nitrogen (NOx) sensing module of the 'Inspector NOxCO' device. 'Inspector NOxCO' is a bacterial sensor for carbon monoxide (CO) and oxides of nitrogen (NOx) in vehicle emissions. This part consists of the Anderson constitutive promoter(BBa_J23110) and RBS(B0030) with the coding sequence for NsrR(K2056001) a NOx sensitive repressor protein and single terminator(B0010) followed by NsrR repressed promoter pYeaR(K216005) with RBS(B0030) and 'Yellow chromoprotein'(K1033910) with terminator(BBa_B0012). If NOx are present, they will bind to the constitutive expressed NsrR that will relieve repression on the pYeaR promoter activating transcription of the yellow chromoprotein. Sequence and Features
discontinued: false
dominant: false
group u list: _2524_
m user id: 33019
owner id: 33019
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The selection of the RBS and Constitutive promoter. Because of limitations of repeats that could be synthesized, we had to give up on the double terminators in the original design and go with single different terminators. A 70bp spacer(B0040) has been added for transcriptional efficiency.
star: false
creator: Muhammad Ismail
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_B0012
component: BBa_K1033910
component: BBa_J23110
component: BBa_K2056001
component: BBa_B0030
component: BBa_B0040
component: BBa_B0010
component: BBa_K216005
sequence annotation: BBa_J23110
sequence annotation: BBa_B0030
sequence annotation: BBa_K2056001
sequence annotation: BBa_B0010
sequence annotation: BBa_B0040
sequence annotation: BBa_K216005
sequence annotation: BBa_K1033910
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K2056005_sequence/1

BBa_B0012
type: Component
persistent identity: component2499370
display id: component2499370
version: 1
top level: BBa_K2056005
definition: BBa_B0012
access: public

BBa_K1033910
type: Component
persistent identity: component2499369
display id: component2499369
version: 1
top level: BBa_K2056005
definition: fwYellow
access: public

BBa_J23110
type: Component
persistent identity: component2499351
display id: component2499351
version: 1
top level: BBa_K2056005
definition: BBa_J23110
access: public

BBa_K2056001
type: Component
persistent identity: component2499355
display id: component2499355
version: 1
top level: BBa_K2056005
definition: BBa_K2056001
access: public

BBa_B0030
type: Component
persistent identity: component2499353
display id: component2499353
version: 1
top level: BBa_K2056005
definition: BBa_B0030
access: public

BBa_B0040
type: Component
persistent identity: component2499359
display id: component2499359
version: 1
top level: BBa_K2056005
definition: spacer
access: public

BBa_B0010
type: Component
persistent identity: component2499356
display id: component2499356
version: 1
top level: BBa_K2056005
definition: BBa_B0010
access: public

BBa_B0030
type: Component
persistent identity: component2499366
display id: component2499366
version: 1
top level: BBa_K2056005
definition: BBa_B0030
access: public

BBa_K216005
type: Component
persistent identity: component2499364
display id: component2499364
version: 1
top level: BBa_K2056005
definition: BBa_K216005
access: public

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2499351
display id: annotation2499351
version: 1
top level: BBa_K2056005
location: https://synbiohub.org/public/igem/BBa_K2056005/annotation2499351/range2499351/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K2056005/annotation2499351/range2499351/1
type: Range
persistent identity: range2499351
display id: range2499351
version: 1
top level: BBa_K2056005
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2499353
display id: annotation2499353
version: 1
top level: BBa_K2056005
location: https://synbiohub.org/public/igem/BBa_K2056005/annotation2499353/range2499353/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K2056005/annotation2499353/range2499353/1
type: Range
persistent identity: range2499353
display id: range2499353
version: 1
top level: BBa_K2056005
direction: direction/forward
start: 44
end: 58
orientation: inline

BBa_K2056001
type: SequenceAnnotation
persistent identity: annotation2499355
display id: annotation2499355
version: 1
top level: BBa_K2056005
location: https://synbiohub.org/public/igem/BBa_K2056005/annotation2499355/range2499355/1
component: BBa_K2056001

https://synbiohub.org/public/igem/BBa_K2056005/annotation2499355/range2499355/1
type: Range
persistent identity: range2499355
display id: range2499355
version: 1
top level: BBa_K2056005
direction: direction/reverse
start: 67
end: 492
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2499356
display id: annotation2499356
version: 1
top level: BBa_K2056005
location: https://synbiohub.org/public/igem/BBa_K2056005/annotation2499356/range2499356/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K2056005/annotation2499356/range2499356/1
type: Range
persistent identity: range2499356
display id: range2499356
version: 1
top level: BBa_K2056005
direction: direction/forward
start: 501
end: 580
orientation: inline

BBa_B0040
type: SequenceAnnotation
persistent identity: annotation2499359
display id: annotation2499359
version: 1
top level: BBa_K2056005
location: https://synbiohub.org/public/igem/BBa_K2056005/annotation2499359/range2499359/1
component: BBa_B0040

https://synbiohub.org/public/igem/BBa_K2056005/annotation2499359/range2499359/1
type: Range
persistent identity: range2499359
display id: range2499359
version: 1
top level: BBa_K2056005
direction: direction/forward
start: 589
end: 658
orientation: inline

BBa_K216005
type: SequenceAnnotation
persistent identity: annotation2499364
display id: annotation2499364
version: 1
top level: BBa_K2056005
location: https://synbiohub.org/public/igem/BBa_K2056005/annotation2499364/range2499364/1
component: BBa_K216005

https://synbiohub.org/public/igem/BBa_K2056005/annotation2499364/range2499364/1
type: Range
persistent identity: range2499364
display id: range2499364
version: 1
top level: BBa_K2056005
direction: direction/reverse
start: 667
end: 766
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2499366
display id: annotation2499366
version: 1
top level: BBa_K2056005
location: https://synbiohub.org/public/igem/BBa_K2056005/annotation2499366/range2499366/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K2056005/annotation2499366/range2499366/1
type: Range
persistent identity: range2499366
display id: range2499366
version: 1
top level: BBa_K2056005
direction: direction/forward
start: 775
end: 789
orientation: inline

BBa_K1033910
type: SequenceAnnotation
persistent identity: annotation2499369
display id: annotation2499369
version: 1
top level: BBa_K2056005
location: https://synbiohub.org/public/igem/BBa_K2056005/annotation2499369/range2499369/1
component: BBa_K1033910

https://synbiohub.org/public/igem/BBa_K2056005/annotation2499369/range2499369/1
type: Range
persistent identity: range2499369
display id: range2499369
version: 1
top level: BBa_K2056005
direction: direction/reverse
start: 796
end: 1509
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2499370
display id: annotation2499370
version: 1
top level: BBa_K2056005
location: https://synbiohub.org/public/igem/BBa_K2056005/annotation2499370/range2499370/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K2056005/annotation2499370/range2499370/1
type: Range
persistent identity: range2499370
display id: range2499370
version: 1
top level: BBa_K2056005
direction: direction/forward
start: 1518
end: 1558
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

fwYellow
type: ComponentDefinition
persistent identity: BBa K1033910
display id: BBa_K1033910
version: 1
was generated by: iGEM to SBOL conversion
description: fwYellow, yellow chromoprotein
created: 2013-09-16T11:00:00Z
modified: 2016-01-25T02:41:53Z
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
mutable description: This chromoprotein naturally exhibits strong color when expressed.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 4206
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This construct is synthetic. It was synthesized from a template where the consensus sequence for the "color dependancy" in order to obtain a chromoprotein with a unique yellow color.
star: false
creator: Sabri Jamal
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: fwYellow
sequence: https://synbiohub.org/public/igem/BBa_K1033910_sequence/1

fwYellow
type: SequenceAnnotation
persistent identity: annotation2371218
display id: annotation2371218
version: 1
top level: fwYellow
location: https://synbiohub.org/public/igem/BBa_K1033910/annotation2371218/range2371218/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1033910/annotation2371218/range2371218/1
type: Range
persistent identity: range2371218
display id: range2371218
version: 1
top level: fwYellow
direction: direction/forward
start: 1
end: 711
orientation: inline

BBa_K2056001
type: ComponentDefinition
persistent identity: BBa K2056001
display id: BBa_K2056001
version: 1
was generated by: iGEM to SBOL conversion
description: NsrR - NO(x) sensitive pYear, pNirk repressor protein
created: 2016-10-12T11:00:00Z
modified: 2016-10-13T10:04:47Z
mutable provenance: This part has been designed after the part Bba_K1682011 in the Registry of Standard Biological Parts.
owned by: james
owned by: myers
mutable description: This part contains the NsrR (NO(x) sensitive pYear, pNirk repressor protein) encoding region. Escherichia coli (E. coli) detects environmental nitrate by the yeaR-yoaG operon. PyeaR is regulated by the NsrR repressor protein. Nitric oxide binds to the NsrR protein and relieves the repression on PyeaR. As a result, any genes that are downstream of PyeaR are expressed. This part is also related with BBa_K2056002, the promoter region of the NirK protein, because NsrR activates the transcription of NirK under high nitrite conditions. Due to these properties, we can use this part as a sensor for oxides of nitrogen.
discontinued: false
dominant: false
group u list: _2524_
m user id: 33019
owner id: 33019
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Because the part Bba_K1682011 was not available from the registry, we had to get it synthesized. We had to codon optimized this part to conform to assembly standards and the requirements for synthesis.
star: false
creator: Muhammad Ismail
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2056001_sequence/1

spacer
type: ComponentDefinition
persistent identity: BBa B0040
display id: BBa_B0040
version: 1
was generated by: iGEM to SBOL conversion
description: Spacer.1 (generic)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Randomly generated and optimized for several parameters (see Design notes).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Generic spacer for ensuring a 70 bp distance between the end of the suffix of the BioBrick part containing the double terminator and the prefix of the BioBrick part containing the promoter of the new gene. Please, use the AlignX function of Vector NT to check for homology with the components in your plasmid before using this spacer.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The size of the spacer was choosed to meet the minimum length of a sequence that can be queried using the BLAST search engine. However, subsequences of it can be used to design shorter spacers. The sequence was selected from many more sequences randomly generated using the Random DNA Generator engine; the GC% parameter used as input was 50%. The sequences were selected based on the following constraints listed in their order of importance: the absence of any putative promoter regions, a low degree of homology with the Elowitz plasmid (whose components are widely used in our designs), no homology with other E.coli sequences as shown by BLASTN search results and the presence of a number of TAA stop codons. The second constraint was the most stringent leading to the elimination of most sequences. DE made the following changes to the original sequence in order to add stop codons in the -3 frame and more in the +2 frame (note, not all of these stop codons are UAA. Thus, if used in an organism that inserts an amino acid @ UGA or UAG the obvious will occur): T->A @ 85 T->A @ 42 C->T @ 79 A->T @ 64 A->T @ 31 T->A @ 34 C->A @ 37 Also, note that the above changes further reduce (the already very weak) homology to current NCBI-stored sequences. In the process of selecting the best sequence it appeared that a good alternative sequence for a spacer would be: AGGTTCTGATATGTAACTGTGCCCAATGTCGTTAGTGACGCATACCTCTTAAGAGGCCACTGTCCTAACA. The sequence contains no putative promoters and shows moderate homology with the 5' end of the Ampicillin resistance gene. However a strong promoter sequence starts 12 bp downstream of this sequence, and therefore the sequence presented above was preferred. The sequence is compatible (does not show significant homology) with the components in the Elowitz repressilator plasmid.
star: true
creator: Vinay S. Mahajan, Brian Chow, Peter Carr
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Spacer-1
sequence annotation: BBa_B0040
sequence: https://synbiohub.org/public/igem/BBa_B0040_sequence/1

Spacer-1
type: SequenceAnnotation
persistent identity: annotation1721
display id: annotation1721
version: 1
top level: spacer
location: https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1
type: Range
persistent identity: range1721
display id: range1721
version: 1
top level: spacer
direction: direction/forward
start: 1
end: 70
orientation: inline

BBa_B0040
type: SequenceAnnotation
persistent identity: annotation7030
display id: annotation7030
version: 1
top level: spacer
location: https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1
type: Range
persistent identity: range7030
display id: range7030
version: 1
top level: spacer
direction: direction/forward
start: 1
end: 70
orientation: inline

BBa_J23110
type: ComponentDefinition
persistent identity: BBa J23110
display id: BBa_J23110
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23110_sequence/1

BBa_K216005
type: ComponentDefinition
persistent identity: BBa K216005
display id: BBa_K216005
version: 1
was generated by: iGEM to SBOL conversion
description: PyeaR promoter, responsive to nitrate, nitrite and nitric oxide
created: 2009-09-24T11:00:00Z
modified: 2015-05-08T01:11:30Z
mutable provenance: BioBricked from E. coli K-12 genomic DNA by the Edinburgh 2009 iGEM team.
owned by: james
owned by: myers
mutable description: PyeaR promoter. This is the promoter of the Escherichia coli yeaR/yoaG operon (see Lin, H.-Y., Bledsoe, P.J., and Stewart, V. 2007. Activation of yeaR-yoaG operon transcription by the nitrate-responsive regulator NarL is independent of oxygen-responsive regulator Fnr in ''Escherichia coli'' K-12. J. Bacteriol. 189, 7539-7548). Unlike other E. coli promoters responding to nitrate and nitrite, this promoter is not repressed under aerobic conditions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _317_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: No special considerations.
star: false
creator: Edinburgh iGEM 2009
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: NarL-binding region
sequence annotation: NsrR-binding region
sequence annotation: promoter -35 region
sequence annotation: promoter -10 region
sequence: https://synbiohub.org/public/igem/BBa_K216005_sequence/1

NarL-binding region
type: SequenceAnnotation
persistent identity: annotation2027072
display id: annotation2027072
version: 1
top level: BBa_K216005
location: https://synbiohub.org/public/igem/BBa_K216005/annotation2027072/range2027072/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K216005/annotation2027072/range2027072/1
type: Range
persistent identity: range2027072
display id: range2027072
version: 1
top level: BBa_K216005
direction: direction/forward
start: 50
end: 65
orientation: inline

NsrR-binding region
type: SequenceAnnotation
persistent identity: annotation2027073
display id: annotation2027073
version: 1
top level: BBa_K216005
location: https://synbiohub.org/public/igem/BBa_K216005/annotation2027073/range2027073/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K216005/annotation2027073/range2027073/1
type: Range
persistent identity: range2027073
display id: range2027073
version: 1
top level: BBa_K216005
direction: direction/forward
start: 58
end: 80
orientation: inline

promoter -35 region
type: SequenceAnnotation
persistent identity: annotation2027074
display id: annotation2027074
version: 1
top level: BBa_K216005
location: https://synbiohub.org/public/igem/BBa_K216005/annotation2027074/range2027074/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K216005/annotation2027074/range2027074/1
type: Range
persistent identity: range2027074
display id: range2027074
version: 1
top level: BBa_K216005
direction: direction/forward
start: 66
end: 71
orientation: inline

promoter -10 region
type: SequenceAnnotation
persistent identity: annotation2027075
display id: annotation2027075
version: 1
top level: BBa_K216005
location: https://synbiohub.org/public/igem/BBa_K216005/annotation2027075/range2027075/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K216005/annotation2027075/range2027075/1
type: Range
persistent identity: range2027075
display id: range2027075
version: 1
top level: BBa_K216005
direction: direction/forward
start: 89
end: 94
orientation: inline

https://synbiohub.org/public/igem/BBa_K2056005_sequence/1
type: Sequence
persistent identity: BBa K2056005 sequence
display id: BBa_K2056005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagctactagagattaaagaggagaaatactagaggtgcagttaacgagtttcactgattacggattacgtgcgctgatctacatggcgtcattgccagaagggcggatgaccagtatttctgaagtgactgacgtctacggcgtctcccgtaatcatatggtcaaaataatcaatcaacttagtcgtgccggctacgtgactgctgttcgtggaaaaaatggcggcattcgcctgggtaaaccggcgagtgcgatacgtattggtgatgtggtgcgcgagctggagcccttatcgctggtgaattgcagcagtgagttttgccacattacacctgcctgtaggttgaaacaggcactttctaaggccgtgcaaagttttcttacggaactggataactacacgcttgccgatttggttgaagagaatcaaccgctttataaattattgctggtggagtgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagaggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaacatactagagttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaattatcaggcgtaccctgaaacgtactagagattaaagaggagaaatactagatgacggcactgactgaaggcgcaaaactgttcgagaaagaaatcccatatatcactgagctggaaggtgacgttgaaggtatgaagtttatcatcaagggtgaaggtaccggtgacgcgagcgtcggtaaagtggatgctcagttcatttgtaccacgggcgacgttccggttccgtggagcacgctggtcaccacgctgacgtatggtgctcagtgctttgccaagtatccgcgccacattgcggatttcttcaaaagctgcatgccggaaggttacgtccaagagcgcaccatcacctttgagggtgatggcgtgttcaagacccgtgcggaagtcacctttgaaaatggcagcgtgtacaaccgtgtaaaactgaacggccagggtttcaagaaggacggccacgtgctgggcaaaaatctggagtttaactttacccctcattgtttgtacatttggggtgaccaagcgaatcatggcctgaagagcgcgttcaaaatcatgcatgagatcaccggctccaaagaggatttcattgttgccgatcacacccaaatgaataccccgattggtggtggtccggtgcacgtgccggagtaccaccacattacgtatcatgttaccctgtctaaagacgtcaccgatcaccgtgaccatttgaacattgttgaggtgatcaaggcagttgacctggagacgtaccgttaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1033910_sequence/1
type: Sequence
persistent identity: BBa K1033910 sequence
display id: BBa_K1033910_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacggcactgactgaaggcgcaaaactgttcgagaaagaaatcccatatatcactgagctggaaggtgacgttgaaggtatgaagtttatcatcaagggtgaaggtaccggtgacgcgagcgtcggtaaagtggatgctcagttcatttgtaccacgggcgacgttccggttccgtggagcacgctggtcaccacgctgacgtatggtgctcagtgctttgccaagtatccgcgccacattgcggatttcttcaaaagctgcatgccggaaggttacgtccaagagcgcaccatcacctttgagggtgatggcgtgttcaagacccgtgcggaagtcacctttgaaaatggcagcgtgtacaaccgtgtaaaactgaacggccagggtttcaagaaggacggccacgtgctgggcaaaaatctggagtttaactttacccctcattgtttgtacatttggggtgaccaagcgaatcatggcctgaagagcgcgttcaaaatcatgcatgagatcaccggctccaaagaggatttcattgttgccgatcacacccaaatgaataccccgattggtggtggtccggtgcacgtgccggagtaccaccacattacgtatcatgttaccctgtctaaagacgtcaccgatcaccgtgaccatttgaacattgttgaggtgatcaaggcagttgacctggagacgtaccgttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2056001_sequence/1
type: Sequence
persistent identity: BBa K2056001 sequence
display id: BBa_K2056001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgcagttaacgagtttcactgattacggattacgtgcgctgatctacatggcgtcattgccagaagggcggatgaccagtatttctgaagtgactgacgtctacggcgtctcccgtaatcatatggtcaaaataatcaatcaacttagtcgtgccggctacgtgactgctgttcgtggaaaaaatggcggcattcgcctgggtaaaccggcgagtgcgatacgtattggtgatgtggtgcgcgagctggagcccttatcgctggtgaattgcagcagtgagttttgccacattacacctgcctgtaggttgaaacaggcactttctaaggccgtgcaaagttttcttacggaactggataactacacgcttgccgatttggttgaagagaatcaaccgctttataaattattgctggtggagtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0040_sequence/1
type: Sequence
persistent identity: BBa B0040 sequence
display id: BBa_B0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23110_sequence/1
type: Sequence
persistent identity: BBa J23110 sequence
display id: BBa_J23110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K216005_sequence/1
type: Sequence
persistent identity: BBa K216005 sequence
display id: BBa_K216005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaattatcaggcgtaccctgaaacg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z