BBa_K2057001
1
BBa_K2057001
Promoter BtI-BtII
2016-10-13T11:00:00Z
2016-10-21T12:09:31Z
Promoter Bti-BtII of the cry1Ac gene is found on a megaplasmid of Bacillus thuringiensis subsp. kurstaki HD73
This is a dual strong promoter that control the expression of Cry1Ac proteins in Bacillus thuringiensis, but it is expressed constitutively in Escherichia coli. This promoter can control the expression of genes that need to be expressed in a constitutive way in E. coli.
false
false
_2525_
30590
21127
9
false
We amplified the promoter from the cry1Ac gene and include restriction sites not found inside the sequence but compatible with the prefix and suffix of the pSB1C3
false
Uriel Eleazar Barboza P??rez
annotation2513451
1
BtII (-10)
range2513451
1
294
302
annotation2513450
1
BtI (-35)
range2513450
1
285
291
annotation2513452
1
BtI (-10)
range2513452
1
306
313
annotation2513453
1
RBS
range2513453
1
377
381
BBa_K2057001_sequence
1
ttacaattcaagatgaattgcaggtaaatggttctaacatgtataagtgtaagtatttctacattaccacaaattctcaatttgtatatgtaaaataggaaaagtggattttatatataagtataaaaagtaataagactttaaaataagttaacggaatacaaacccttaatgcattggttaaacattgtaaagtctaaagcatggataatgggcgagaagtaagtagattgttaacaccctgggtcaaaaattgatatttagtaaaattagttgcactttgtgcattttttcataagatgagtcatatgttttaaattgtagtaatgaaaaacagtattatatcataatgaattggtatcttaataaaagagatggaggtaactt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z