BBa_K2057000 1 BBa_K2057000 Promoter chiA74 from Bacillus thuringiensis 2016-10-13T11:00:00Z 2016-10-20T10:14:33Z Promoter chiA74 is found on the chromosome of a mexican strain of Bacillus thuringiensis subsp. kenyae (Gen bank accession number AF424979) The promoter chiA74 control the expression of the endochitinase chiA74 from a strain of Bacillus thuringiensis. In spite of is derived from a gene of a Gram positive bacterium, it is funcional in a Gram negative bacteria, and control the expression of the same gene in Escherichia coli in a constitutive form. This promoter can be to control the expression constitutively of a gene in E. coli. false false _2525_ 21127 21127 9 false The only modification was that we included two artificial restriction sites in the in the 5??and 3??terminus, so that we could clone it in a vector. false Uriel Eleazar Barboza P??rez annotation2525733 1 -10 promoter range2525733 1 399 404 annotation2525732 1 -35 promoter range2525732 1 379 384 BBa_K2057003 1 BBa_K2057003 LasR under the regulation of the promoter chiA74 2016-10-16T11:00:00Z 2016-10-17T08:44:04Z Both regulatory elements come from regulatory elements The promoter chiA74 will regulate the expression of the LasR false false _2525_ 30590 30590 9 false We previously tested the functionality of the promoter chiA74 in E. coli false Jos?? Eleazar Barboza Corona component2514499 1 BBa_K2057000 component2514504 1 BBa_C0079 annotation2514499 1 BBa_K2057000 range2514499 1 1 568 annotation2514504 1 BBa_C0079 range2514504 1 575 1355 BBa_C0079 1 lasr lasR activator from P. aeruginosa PAO1(+LVA) 2004-01-27T12:00:00Z 2015-08-31T04:07:24Z Genbank: NC_002516 (www.ncbi.nlm.nih.gov) Released HQ 2013 coding region for lasR protein, which accepts chemical signal AI-1 (made by lasI protein) false false _1_ 0 24 7 In stock false Mutated 480 from g to a to remove PstI site true Alvin Carter Powers (Fighting Darwins) annotation2213999 1 Help:Barcodes range2213999 1 757 781 annotation307935 1 lasR range307935 1 1 717 annotation308006 1 G range308006 1 480 480 annotation307948 1 LVA range307948 1 718 750 BBa_K2057003_sequence 1 tttaatatatctttttgtagttccatttctggtggaatcattcccgcattttttaaaattttataactcattctaagttctggaggtaccattgaaagatcttctaattgtagtggttttccttttcccggaagataatcaagatcaccattccgtattgcttgtcgaattttttcttcagcaatgttcaaaaacacatccacactacttccctccttgacatatttttactattatttcacatgatatttccttatctttctacgtctttaataattggctccatacaattttttctcaaatttatttataatgagctatttcctcccataccaatctttcgttttcatatatagtttgtattcaagcctttttgtattgagaaagtctttttcaacttaataaagcgtttacactaatcttacatttgttacgatttaatcacccccagctcccttgtatagacttcgtgatgtctgatcattttatctagacgtttcacacgtctagattcattttgatttattggcgtatgcctttaaatatatcttttattttgaaaggagaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac BBa_K2057000_sequence 1 tttaatatatctttttgtagttccatttctggtggaatcattcccgcattttttaaaattttataactcattctaagttctggaggtaccattgaaagatcttctaattgtagtggttttccttttcccggaagataatcaagatcaccattccgtattgcttgtcgaattttttcttcagcaatgttcaaaaacacatccacactacttccctccttgacatatttttactattatttcacatgatatttccttatctttctacgtctttaataattggctccatacaattttttctcaaatttatttataatgagctatttcctcccataccaatctttcgttttcatatatagtttgtattcaagcctttttgtattgagaaagtctttttcaacttaataaagcgtttacactaatcttacatttgttacgatttaatcacccccagctcccttgtatagacttcgtgatgtctga BBa_C0079_sequence 1 atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z