BBa_K2057001 1 BBa_K2057001 Promoter BtI-BtII 2016-10-13T11:00:00Z 2016-10-21T12:09:31Z Promoter Bti-BtII of the cry1Ac gene is found on a megaplasmid of Bacillus thuringiensis subsp. kurstaki HD73 This is a dual strong promoter that control the expression of Cry1Ac proteins in Bacillus thuringiensis, but it is expressed constitutively in Escherichia coli. This promoter can control the expression of genes that need to be expressed in a constitutive way in E. coli. false false _2525_ 30590 21127 9 false We amplified the promoter from the cry1Ac gene and include restriction sites not found inside the sequence but compatible with the prefix and suffix of the pSB1C3 false Uriel Eleazar Barboza P??rez annotation2513450 1 BtI (-35) range2513450 1 285 291 annotation2513452 1 BtI (-10) range2513452 1 306 313 annotation2513453 1 RBS range2513453 1 377 381 annotation2513451 1 BtII (-10) range2513451 1 294 302 BBa_K2057004 1 BBa_K2057004 LasR under the regulation of the promoter BtI-BtII 2016-10-16T11:00:00Z 2016-10-17T09:14:52Z Promoter BtI-BtII is harbor in a megaplasmid of B. thuringiensis, lasR was obtained from a biobrick. The promoter BtII from Bacillus thuringiensis regulate the expression of the cry genes during sporulation, but it is functional in Escherichia coli in a constitutive form. false false _2525_ 30590 30590 9 false As promoter is not available in the biobricks, we amplified, sequenced and characterized using the cry1Ac gene as template. false Jos?? Eleazar Barboza Corona component2514526 1 BBa_C0079 component2514521 1 BBa_K2057001 annotation2514521 1 BBa_K2057001 range2514521 1 1 387 annotation2514526 1 BBa_C0079 range2514526 1 394 1174 BBa_C0079 1 lasr lasR activator from P. aeruginosa PAO1(+LVA) 2004-01-27T12:00:00Z 2015-08-31T04:07:24Z Genbank: NC_002516 (www.ncbi.nlm.nih.gov) Released HQ 2013 coding region for lasR protein, which accepts chemical signal AI-1 (made by lasI protein) false false _1_ 0 24 7 In stock false Mutated 480 from g to a to remove PstI site true Alvin Carter Powers (Fighting Darwins) annotation2213999 1 Help:Barcodes range2213999 1 757 781 annotation307948 1 LVA range307948 1 718 750 annotation307935 1 lasR range307935 1 1 717 annotation308006 1 G range308006 1 480 480 BBa_C0079_sequence 1 atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac BBa_K2057001_sequence 1 ttacaattcaagatgaattgcaggtaaatggttctaacatgtataagtgtaagtatttctacattaccacaaattctcaatttgtatatgtaaaataggaaaagtggattttatatataagtataaaaagtaataagactttaaaataagttaacggaatacaaacccttaatgcattggttaaacattgtaaagtctaaagcatggataatgggcgagaagtaagtagattgttaacaccctgggtcaaaaattgatatttagtaaaattagttgcactttgtgcattttttcataagatgagtcatatgttttaaattgtagtaatgaaaaacagtattatatcataatgaattggtatcttaataaaagagatggaggtaactt BBa_K2057004_sequence 1 ttacaattcaagatgaattgcaggtaaatggttctaacatgtataagtgtaagtatttctacattaccacaaattctcaatttgtatatgtaaaataggaaaagtggattttatatataagtataaaaagtaataagactttaaaataagttaacggaatacaaacccttaatgcattggttaaacattgtaaagtctaaagcatggataatgggcgagaagtaagtagattgttaacaccctgggtcaaaaattgatatttagtaaaattagttgcactttgtgcattttttcataagatgagtcatatgttttaaattgtagtaatgaaaaacagtattatatcataatgaattggtatcttaataaaagagatggaggtaactttactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z