BBa_K2057005 1 BBa_K2057005 Expression of gfp under the regulation of promoter ChiA74 2016-10-16T11:00:00Z 2016-10-17T01:44:17Z Promoter chiA74 is part of the chiA74 gene locate on the chromosome of a strain of Bacillus thuringiensis. The gfp is obtained from the biobrick BBa_E0040 We made this composite part to check the functionality of the promoter chiA74 in Escherichia coli false false _2525_ 30590 30590 9 false We need to amplify the promoter chiA74 as it was not available in the biobricks. false Jos?? Eleazar Barboza Corona component2514872 1 BBa_K2057000 component2514874 1 BBa_E0040 annotation2514874 1 BBa_E0040 range2514874 1 575 1294 annotation2514872 1 BBa_K2057000 range2514872 1 1 568 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K2057000 1 BBa_K2057000 Promoter chiA74 from Bacillus thuringiensis 2016-10-13T11:00:00Z 2016-10-20T10:14:33Z Promoter chiA74 is found on the chromosome of a mexican strain of Bacillus thuringiensis subsp. kenyae (Gen bank accession number AF424979) The promoter chiA74 control the expression of the endochitinase chiA74 from a strain of Bacillus thuringiensis. In spite of is derived from a gene of a Gram positive bacterium, it is funcional in a Gram negative bacteria, and control the expression of the same gene in Escherichia coli in a constitutive form. This promoter can be to control the expression constitutively of a gene in E. coli. false false _2525_ 21127 21127 9 false The only modification was that we included two artificial restriction sites in the in the 5??and 3??terminus, so that we could clone it in a vector. false Uriel Eleazar Barboza P??rez annotation2525732 1 -35 promoter range2525732 1 379 384 annotation2525733 1 -10 promoter range2525733 1 399 404 BBa_K2057005_sequence 1 tttaatatatctttttgtagttccatttctggtggaatcattcccgcattttttaaaattttataactcattctaagttctggaggtaccattgaaagatcttctaattgtagtggttttccttttcccggaagataatcaagatcaccattccgtattgcttgtcgaattttttcttcagcaatgttcaaaaacacatccacactacttccctccttgacatatttttactattatttcacatgatatttccttatctttctacgtctttaataattggctccatacaattttttctcaaatttatttataatgagctatttcctcccataccaatctttcgttttcatatatagtttgtattcaagcctttttgtattgagaaagtctttttcaacttaataaagcgtttacactaatcttacatttgttacgatttaatcacccccagctcccttgtatagacttcgtgatgtctgatcattttatctagacgtttcacacgtctagattcattttgatttattggcgtatgcctttaaatatatcttttattttgaaaggagaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K2057000_sequence 1 tttaatatatctttttgtagttccatttctggtggaatcattcccgcattttttaaaattttataactcattctaagttctggaggtaccattgaaagatcttctaattgtagtggttttccttttcccggaagataatcaagatcaccattccgtattgcttgtcgaattttttcttcagcaatgttcaaaaacacatccacactacttccctccttgacatatttttactattatttcacatgatatttccttatctttctacgtctttaataattggctccatacaattttttctcaaatttatttataatgagctatttcctcccataccaatctttcgttttcatatatagtttgtattcaagcctttttgtattgagaaagtctttttcaacttaataaagcgtttacactaatcttacatttgttacgatttaatcacccccagctcccttgtatagacttcgtgatgtctga BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z