BBa_K206030
1
BBa_K206030
Lock 1
2009-05-10T11:00:00Z
2015-05-08T01:11:24Z
Taken from Isaacs et al., with modifications to remove an extra EcoRI site.
crR12-loop-RBS sequence taken from Isaacs et al. (1), with modifications to remove an extra EcoRI site. crR12 binds to the RBS, preventing translation of the mRNA unless a matching "key" RNA is also present in the cell.
1) Isaacs, FJ, Dwyer, DJ, Ding, C, Pervouchine, DD, Cantor, CR, and Collins, JJ. Engineered riboregulators enable post-transcriptional control of gene expression. 2004. Nature Biotechnology 22(7):841-847.
false
false
_307_
0
4172
9
Not in stock
false
Sequence provided in the supplementary table of Isaacs paper is lacking 5 bases present in the diagram that appear to contribute to secondary structure folding. These bases were included in the sequence, with one A->T change to avoid the inclusion of an EcoRI site.
false
Amelia Hardjasa and Alex Ng
annotation2004378
1
crR12
range2004378
1
7
25
annotation2004379
1
loop
range2004379
1
26
31
annotation2004380
1
rbs
range2004380
1
37
44
BBa_K206030_sequence
1
aagcgcagtaattcacctcttggatttgggtatcaaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z