LOCUS       BBa_K2061000            1969 bp    DNA     linear       25-Nov-2024
DEFINITION  tGFP ORF under EF1a promoter
ACCESSION   BBa_K2061000
VERSION     BBa_K2061000.1  
FEATURES             Location/Qualifiers
     promoter        6..1155
                     /label=EF1a promoter
     misc_feature    1192..1950
                     /label=tGFP
     misc_feature    1192..1194
                     /label=start codon
     misc_feature    1948..1950
                     /label=stop codon
     misc_feature    1..6
                     /label=EcoRI site
     misc_feature    1985..1990
                     /label=XbaI site
ORIGIN
        1 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
       61 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
      121 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
      181 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
      241 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
      301 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
      361 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
      421 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
      481 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
      541 agatctgcac actggtattt cggtttttga tgccgcgtac ggcgacgggg cccgtgcgtc
      601 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
      661 gtagtctcaa gcttacggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
      721 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
      781 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
      841 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
      901 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
      961 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     1021 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     1081 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     1141 caggtgtcgt gactataggg cggccggacg tgacaaatgg aagtagcacg cctcactagg
     1201 ctcgtgcaga tggacagcac cgctgcagcc atggagagcg acgagagcgg cctgcccgcc
     1261 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc
     1321 ggcggagagg gcacccccga gcagggccgc atgaccaaca agatgaagag caccaaaggc
     1381 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc
     1441 ggcacctacc ctagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac
     1501 accaacaccc gcatcgagaa gtacgaggac ggtggagtgc tgcacgtgag cttcagctat
     1561 cgctacgagg caggccgcgt gatcggcgac ttcaaggtga tgggcactgg cttccccgag
     1621 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac
     1681 cccatgggcg ataacgatct ggatggcagc ttcacccgca ccttcagcct gcgcgacggc
     1741 ggctactaca gctccgtggt ggacagccac atgcacttca agagcgccat ccaccccagc
     1801 atcctgcaga acggaggtcc tatgttcgcc ttccgccgcg tcgaggagga tcacagcaac
     1861 accgagctag gcatcgtgga gtaccagcac gccttcaaga ccccggatgc agatgccggt
     1921 gaagaaagag tttaatcgat gatatcagat ccactgtatg cagacattg
//