BBa_K2061002 1 BBa_K2061002 gRNA1 for editing F508 deletion 2016-08-22T11:00:00Z 2016-10-10T04:39:15Z synthetic production of the part This plasmid contains the sequence coding for the gRNA for editing the CFTR F508 deletion under U6 promoter. The part was cloned into the pSB1C3 plasmid with chloramphenicol rresistance. false false _2529_ 31098 31098 9 false the part cloned between EcoRI and PstI sites. false avi matityahu annotation2481541 1 PstI site range2481541 1 466 471 annotation2481542 1 U6 range2481542 1 7 324 annotation2481545 1 gRNA scaffold seq range2481545 1 349 425 annotation2481544 1 PAM range2481544 1 346 348 annotation2481540 1 EcoRI site range2481540 1 1 6 annotation2481543 1 Ex11 gRNA1 range2481543 1 326 345 BBa_K2061002_sequence 1 gaattctgtacaaaaaagcaggctttaaaggaaccaattcagtcgactggatccggtaccaaggtcgggcaggaagagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattagaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacgaaacaccgaccattaaagaaaatatcattgggttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttttctagacccagctttcttgtacaaagttggcattactgcag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z