LOCUS       BBa_K2061003             471 bp    DNA     linear       22-Dec-2024
DEFINITION  gRNA2 for editing F508 deletion
ACCESSION   BBa_K2061003
VERSION     BBa_K2061003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=EcorI site
     promoter        7..324
                     /label=U6
     CDS             326..345
                     /label=Ex11 gRNA2
     misc_feature    346..348
                     /label=PAM
     CDS             349..425
                     /label=gRNA scaffold seq
     misc_feature    466..471
                     /label=PstI site
ORIGIN
        1 gaattctgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg atccggtacc
       61 aaggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca tatacgatac
      121 aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa
      181 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
      241 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
      301 atcttgtgga aaggacgaaa caccgcaaag catgccaact agaagagggt tttagagcta
      361 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
      421 gtgctttttt tctagaccca gctttcttgt acaaagttgg cattactgca g
//