BBa_K2061005 1 LTBD Heat Labile Toxin B subunit with DNA binding Domain 2016-10-13T11:00:00Z 2016-10-17T02:36:45Z LTB comes from E.coli DBD comes from AV1 virus A new chimeric protein that include both DNA Binding Domain (DBD) and Heat Labile Toxin B (LTB) connected via a linker. LTB is an enterotoxin from E.coli, not toxic. LTB can used as a homing device, since it can bind, with high affinity (KD 10-10 ??? 10-12 M), to the various ganglioside molecules present on the cell membrane of all epithelial cells. DBD, wass originally taken from a virus, has the ability to bind DNA. LTB+DBD can used as a delivery system, in vivo, for DNA into Epithelial cell tissue. false false _2529_ 30576 30576 9 false LTB and DBD have to include in betwwen a liner of ~20 amino acid long. false Almog Angel BBa_K2061005_sequence 1 atggctcctcagtctattacagaactatgttcggaatatcgcaacacacaaatatatacgataaatgacaagatactatcatatacggaatcgatggcaggcaaaagagaaatggttatcattacatttaagagcggcgcaacatttcaggtcgaagtcccgggcagtcaacatatagactcccaaaaaaaagccattgaaaggatgaaggacacattaagaatcacatatctgaccgagaccaaaattgataaattatgtgtatggaataataaaacccccaattcaattgcggcaatcagtatggaaaacggcggcggcggcagcggcggcggcggcagcggcggcggcggcagcaaaaaaaacaaaaaaaaaaacaaaaaaaaaaacaaaaaaaaaaacaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z