BBa_K2064001
1
BBa_K2064001
TyrA Donor COM
2016-10-20T11:00:00Z
2016-10-21T04:33:18Z
Synthetic, codon optimized for E. coli
We developed a BioBrick that is used to link the inserted protein with a protein containing a Tyrocidine B acceptor domain. This allows the two proteins to come together in physical space through protein-protein interactions and increase the efficiency of enzymatic gene clusters. It is under the control of an inducible T7 promoter and terminator pair from a pET-16b vector allowing expression of protein. It contains a golden gate cloning site for the scarless insertion of NRPS modules or other proteins.
false
false
_2532_
27296
27296
9
false
Xba1 cut site removed from the region downstream of the promoter
false
Dragos S. Chiriac
BBa_K2064001_sequence
1
cgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctcttgatataattttgtttaactttaagaaggagatatagcatggagaccgctggcacgacagttggtctcccatcagacttttcagtaaaaggtttgcagatggaagagatggatgatattttcgagttactggcaaacacgttgagataataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z