BBa_K2065001 1 BBa_K2065001 CT52-mNeonGreen 2016-09-25T11:00:00Z 2016-10-18T02:49:37Z - CT52 is linked to mNeonGreen by a flexible GGS-10 linker to make sure the mNeonGreen is able to move towards other bound proteins. This insert is placed into a pET28a vector and expressed by the E. Coli BL21 (DE3) bacteria. The part is used for BRET assays in the tetrameric scaffold together with the CT52-SsNL and CT52-LsNL. mNeonGreen-CT52 will be the acceptor that changes the wavelength of the light emitted by NanoLuc to a wavelength of 517 nm. The sequence of our CT52-mNeonGreen has been verified by Starseq. It contains the prefix and suffix with the correct restriction sites (EcoRI, XbaI, SpeI and PstI). mNeonGreen-CT52 is 956 bp long. false false _2533_ 30740 30740 9 false - false Tom van Sonsbeek BBa_K2065001_sequence 1 atgcttatggtgagtaagggcgaggaggataacatggcctctctcccagcgacacatgagttacacatctttggctccatcaacggtgtggactttgacatggtgggtcagggcaccggcaatccaaatgatggttatgaggagttaaacctgaagtccaccaagggtgacctccagttctccccctggattctggtccctcatatcgggtatggcttccatcagtacctgccctaccctgacgggatgtcgcctttccaggccgccatggtagatggctccggataccaagtccatcgcacaatgcagtttgaagatggtgcctcccttactgttaactaccgctacacctacgagggaagccacatcaaaggagaggcccaggtgaaggggactggtttccctgctgacggtcctgtgatgaccaactcgctgaccgctgcggactggtgcaggtcgaagaagacttaccccaacgacaaaaccatcatcagtacctttaagtggagttacaccactggaaatggcaagcgctaccggagcactgcgcggaccacctacacctttgccaagccaatggcggctaactatctgaagaaccagccgatgtacgtgttccgtaagacggagctcaagcactccaagaccgagctcaacttcaaggagtggcaaaaggcctttaccgatgtgatgggcatggacgagctgtacaagggtggtagtggtggatctggagggtccggcggttccggtggtagtggtggctctgggggtagcggcggtagcggtgggtctggcggtagtacaaactttaacgaattaaaccaactggccgaggaagctaagcgcagggccgaaatcgcacggcagcgtgaattacacaccttaaaaggacatgtagaaagtgtagttaaactcaaaggtctggatatcgaaacaatccaacaaagttacgatatataaggatcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z