LOCUS       BBa_K2066022             963 bp    DNA     linear       17-May-2024
DEFINITION  Constitutive tetR + UNS
ACCESSION   BBa_K2066022
VERSION     BBa_K2066022.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     promoter        41..75
                     /label=BBa_J23105
     misc_feature    76..83
                     /label=BBa_K2066529
     RBS             84..101
                     /label=BBa_K2066527
     CDS             105..721
                     /label=tetR
     misc_feature    722..755
                     /label=SsrA
     misc_feature    762..786
                     /label=Help:Barcodes
     CDS             102..786
                     /label=BBa_C0040
     misc_feature    787..794
                     /label=BBa_K2066529
     stem_loop       806..849
                     /label=stem_loop
     misc_feature    795..874
                     /label=BBa_B0010
     terminator      795..874
                     /label=BBa_B0010
     stem_loop       890..909
                     /label=T7 TE
     misc_feature    916..916
                     /label=stop
     polyA_site      910..923
                     /label=polya
     misc_feature    883..923
                     /label=BBa_B0012
     terminator      883..923
                     /label=BBa_B0012
     terminator      795..923
                     /label=BBa_B0015
     misc_feature    924..963
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc tttacggcta gctcagtcct
       61 aggtactatg ctagctacta gagaaagagg agaaatacta gatgtccaga ttagataaaa
      121 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa
      181 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa
      241 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact
      301 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta
      361 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag
      421 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac
      481 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg
      541 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc
      601 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct
      661 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt
      721 ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag tgctagtgta
      781 gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      841 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      901 gggtgggcct ttctgcgttt atagcactga aggtcctcaa tcgcactgga aacatcaagg
      961 tcg
//