BBa_K2066022
type: ComponentDefinition
persistent identity: BBa K2066022
display id: BBa_K2066022
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive tetR + UNS
created: 2016-08-28T11:00:00Z
modified: 2016-10-12T12:59:33Z
mutable provenance: This part was synthesized using different parts from the iGEM registry.
owned by: james
owned by: myers
mutable description: This is a composite part with a constitutive Anderson promoter J23105 to express TetR repressor. Part used to express the repressor TetR that can bind to pTET (promoter) to block the expression of GFP (or any gene that pTET controls). The tetR repressor protein can be temporarily blocked by aTc induction to allow for transcription of gene product controlled by pTET.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 27446
owner id: 31541
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Part was created to put Bba_I739001 on a UNS backbone.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K2066019
component: BBa_B0015
component: BBa_K2066529
component: BBa_C0040
component: BBa_K2066527
component: BBa_J23105
component: BBa_K2066018
sequence annotation: BBa_K2066018
sequence annotation: BBa_J23105
sequence annotation: BBa_K2066529
sequence annotation: BBa_K2066527
sequence annotation: BBa_C0040
sequence annotation: BBa_B0015
sequence annotation: BBa_K2066019
sequence: https://synbiohub.org/public/igem/BBa_K2066022_sequence/1

BBa_K2066019
type: Component
persistent identity: component2494831
display id: component2494831
version: 1
top level: BBa_K2066022
definition: BBa_K2066019
access: public

BBa_B0015
type: Component
persistent identity: component2494830
display id: component2494830
version: 1
top level: BBa_K2066022
definition: BBa_B0015
access: public

BBa_K2066529
type: Component
persistent identity: component2494823
display id: component2494823
version: 1
top level: BBa_K2066022
definition: BBa_K2066529
access: public

BBa_C0040
type: Component
persistent identity: component2494822
display id: component2494822
version: 1
top level: BBa_K2066022
definition: tetR
access: public

BBa_K2066527
type: Component
persistent identity: component2494818
display id: component2494818
version: 1
top level: BBa_K2066022
definition: BBa_K2066527
access: public

BBa_K2066529
type: Component
persistent identity: component2494817
display id: component2494817
version: 1
top level: BBa_K2066022
definition: BBa_K2066529
access: public

BBa_J23105
type: Component
persistent identity: component2494816
display id: component2494816
version: 1
top level: BBa_K2066022
definition: BBa_J23105
access: public

BBa_K2066018
type: Component
persistent identity: component2494815
display id: component2494815
version: 1
top level: BBa_K2066022
definition: BBa_K2066018
access: public

BBa_K2066018
type: SequenceAnnotation
persistent identity: annotation2494815
display id: annotation2494815
version: 1
top level: BBa_K2066022
location: https://synbiohub.org/public/igem/BBa_K2066022/annotation2494815/range2494815/1
component: BBa_K2066018

https://synbiohub.org/public/igem/BBa_K2066022/annotation2494815/range2494815/1
type: Range
persistent identity: range2494815
display id: range2494815
version: 1
top level: BBa_K2066022
direction: direction/reverse
start: 1
end: 40
orientation: inline

BBa_J23105
type: SequenceAnnotation
persistent identity: annotation2494816
display id: annotation2494816
version: 1
top level: BBa_K2066022
location: https://synbiohub.org/public/igem/BBa_K2066022/annotation2494816/range2494816/1
component: BBa_J23105

https://synbiohub.org/public/igem/BBa_K2066022/annotation2494816/range2494816/1
type: Range
persistent identity: range2494816
display id: range2494816
version: 1
top level: BBa_K2066022
direction: direction/reverse
start: 41
end: 75
orientation: inline

BBa_K2066529
type: SequenceAnnotation
persistent identity: annotation2494817
display id: annotation2494817
version: 1
top level: BBa_K2066022
location: https://synbiohub.org/public/igem/BBa_K2066022/annotation2494817/range2494817/1
component: BBa_K2066529

https://synbiohub.org/public/igem/BBa_K2066022/annotation2494817/range2494817/1
type: Range
persistent identity: range2494817
display id: range2494817
version: 1
top level: BBa_K2066022
direction: direction/reverse
start: 76
end: 83
orientation: inline

BBa_K2066527
type: SequenceAnnotation
persistent identity: annotation2494818
display id: annotation2494818
version: 1
top level: BBa_K2066022
location: https://synbiohub.org/public/igem/BBa_K2066022/annotation2494818/range2494818/1
component: BBa_K2066527

https://synbiohub.org/public/igem/BBa_K2066022/annotation2494818/range2494818/1
type: Range
persistent identity: range2494818
display id: range2494818
version: 1
top level: BBa_K2066022
direction: direction/reverse
start: 84
end: 101
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2494822
display id: annotation2494822
version: 1
top level: BBa_K2066022
location: https://synbiohub.org/public/igem/BBa_K2066022/annotation2494822/range2494822/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K2066022/annotation2494822/range2494822/1
type: Range
persistent identity: range2494822
display id: range2494822
version: 1
top level: BBa_K2066022
direction: direction/reverse
start: 102
end: 786
orientation: inline

BBa_K2066529
type: SequenceAnnotation
persistent identity: annotation2494823
display id: annotation2494823
version: 1
top level: BBa_K2066022
location: https://synbiohub.org/public/igem/BBa_K2066022/annotation2494823/range2494823/1
component: BBa_K2066529

https://synbiohub.org/public/igem/BBa_K2066022/annotation2494823/range2494823/1
type: Range
persistent identity: range2494823
display id: range2494823
version: 1
top level: BBa_K2066022
direction: direction/reverse
start: 787
end: 794
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2494830
display id: annotation2494830
version: 1
top level: BBa_K2066022
location: https://synbiohub.org/public/igem/BBa_K2066022/annotation2494830/range2494830/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2066022/annotation2494830/range2494830/1
type: Range
persistent identity: range2494830
display id: range2494830
version: 1
top level: BBa_K2066022
direction: direction/reverse
start: 795
end: 923
orientation: inline

BBa_K2066019
type: SequenceAnnotation
persistent identity: annotation2494831
display id: annotation2494831
version: 1
top level: BBa_K2066022
location: https://synbiohub.org/public/igem/BBa_K2066022/annotation2494831/range2494831/1
component: BBa_K2066019

https://synbiohub.org/public/igem/BBa_K2066022/annotation2494831/range2494831/1
type: Range
persistent identity: range2494831
display id: range2494831
version: 1
top level: BBa_K2066022
direction: direction/reverse
start: 924
end: 963
orientation: inline

BBa_K2066018
type: ComponentDefinition
persistent identity: BBa K2066018
display id: BBa_K2066018
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 2 Sequence, from Torella et al., 2013
created: 2016-07-11T11:00:00Z
modified: 2016-10-19T05:41:43Z
mutable provenance: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
owned by: james
owned by: myers
mutable description: This is Unique Nucleotide Sequence 2, (UNS 2), from Torella et al., 2013. The William and Mary iGEM team has adopted this as our standard prefix; as such, all of our parts will have this sequence immediately following the BioBrick prefix. We took this measure in order to allow easier Gibson Assembly cloning of our parts. Primer sequences which can be used to clone with the UNS 2/3 standard can be found on our wiki.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: UNS 2 was chosen because it works well with UNS 3 and it is in accordance with the BioBrick standard.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K2066018_sequence/1

BBa_K2066019
type: ComponentDefinition
persistent identity: BBa K2066019
display id: BBa_K2066019
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 3 Sequence, from Torella et al., 2013
created: 2016-07-11T11:00:00Z
modified: 2016-10-19T05:43:00Z
mutable provenance: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
owned by: james
owned by: myers
mutable description: This is Unique Nucleotide Sequence 3, (UNS 3), from Torella et al., 2013. The William and Mary iGEM team has adopted this as our standard prefix; as such, all of our parts will have this sequence immediately following the BioBrick prefix. We took this measure in order to allow easier Gibson Assembly cloning of our parts. Primer sequences which can be used to clone with the UNS 2/3 standard can be found on our wiki. The sequence for this part came from the following paper: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860. A huge thanks to all the researchers involved in its original creation!
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This UNS sequence was chosen to serve as the 3' primer in our standard because it works well with UNS 2 and it adheres to the BioBrick standards.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066019_sequence/1

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_J23105
type: ComponentDefinition
persistent identity: BBa J23105
display id: BBa_J23105
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23105_sequence/1

BBa_K2066527
type: ComponentDefinition
persistent identity: BBa K2066527
display id: BBa_K2066527
version: 1
was generated by: iGEM to SBOL conversion
description: B0034 with scar TACTAG
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T11:39:15Z
mutable provenance: Bba_B0034 + TACTAG
owned by: james
owned by: myers
mutable description: This is part B0034 with the scar region TACTAG added onto the part so that the correct scar region will be present in WM composite parts K2066059 - K20660108. This has no other purpose, but is just necessary do the system of the registry.
discontinued: false
dominant: false
group u list: _2534_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Joseph L Maniaci
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K2066527_sequence/1

BBa_K2066529
type: ComponentDefinition
persistent identity: BBa K2066529
display id: BBa_K2066529
version: 1
was generated by: iGEM to SBOL conversion
description: TACTAGAG scar
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T12:58:59Z
mutable provenance: n/a
owned by: james
owned by: myers
mutable description: TACTAGAG scar, only exists for bookkeeping because scars were being incorrectly added to our composite parts
discontinued: false
dominant: false
group u list: _2534_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: n/a
star: false
creator: Joseph L Maniaci
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K2066529_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K2066022_sequence/1
type: Sequence
persistent identity: BBa K2066022 sequence
display id: BBa_K2066022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgggagttcgtagacggaaacaaacgcagaatccaagctttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagcactgaaggtcctcaatcgcactggaaacatcaaggtcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066018_sequence/1
type: Sequence
persistent identity: BBa K2066018 sequence
display id: BBa_K2066018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgggagttcgtagacggaaacaaacgcagaatccaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066019_sequence/1
type: Sequence
persistent identity: BBa K2066019 sequence
display id: BBa_K2066019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcactgaaggtcctcaatcgcactggaaacatcaaggtcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23105_sequence/1
type: Sequence
persistent identity: BBa J23105 sequence
display id: BBa_J23105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtactatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066527_sequence/1
type: Sequence
persistent identity: BBa K2066527 sequence
display id: BBa_K2066527_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066529_sequence/1
type: Sequence
persistent identity: BBa K2066529 sequence
display id: BBa_K2066529_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z