LOCUS       BBa_K2066023            1017 bp    DNA     linear       25-Nov-2024
DEFINITION  pTet GFP + UNS
ACCESSION   BBa_K2066023
VERSION     BBa_K2066023.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     misc_feature    41..59
                     /label=TetR 1
     misc_feature    41..94
                     /label=BBa_R0040
     promoter        60..65
                     /label=-35
     misc_feature    66..84
                     /label=TetR 2
     promoter        83..88
                     /label=-10
     promoter        41..94
                     /label=BBa_R0040
     misc_feature    95..102
                     /label=BBa_K2066529
     RBS             103..120
                     /label=BBa_K2066527
     CDS             121..840
                     /label=GFP protein
     CDS             121..840
                     /label=BBa_E0040
     misc_feature    841..848
                     /label=BBa_K2066529
     stem_loop       860..903
                     /label=stem_loop
     misc_feature    849..928
                     /label=BBa_B0010
     terminator      849..928
                     /label=BBa_B0010
     stem_loop       944..963
                     /label=T7 TE
     misc_feature    970..970
                     /label=stop
     polyA_site      964..977
                     /label=polya
     misc_feature    937..977
                     /label=BBa_B0012
     terminator      937..977
                     /label=BBa_B0012
     terminator      849..977
                     /label=BBa_B0015
     misc_feature    978..1017
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc tccctatcag tgatagagat
       61 tgacatccct atcagtgata gagatactga gcactactag agaaagagga gaaatactag
      121 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      181 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      241 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      301 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      361 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      421 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      481 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      541 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      601 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      661 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      721 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      781 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
      841 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      901 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      961 gcctttctgc gtttatagca ctgaaggtcc tcaatcgcac tggaaacatc aaggtcg
//