BBa_K2066025
type: ComponentDefinition
persistent identity: BBa K2066025
display id: BBa_K2066025
version: 1
was generated by: iGEM to SBOL conversion
description: pTac+RiboJ+cIGFP
created: 2016-08-31T11:00:00Z
modified: 2016-10-28T08:36:39Z
mutable provenance: The RBS, RiboJ, and reporter sequences are from Lou et. al. 2012. The UNS sequences are from Torella et. al 2013.
owned by: james
owned by: myers
mutable description: This part is used for 2016 WM iGEM Ribozyme Characterization project. Lou et. al showed that sequences in the 5' UTR of a gene can affect the input-output relationship (transfer function) of a circuit. The RiboJ serves as an insulator to generalize the transfer function of a circuit regardless of promoter. This part will be used in concert with WM16_024 (pTac Ribozyme Chracterization Part ???GFP Only) to determine the influence of the RiboJ insulator sequence on gene expression.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 31541
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This part follows Lou et al. 2012 (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???).
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K2066018
component: BBa_K2066506
component: BBa_K864400
component: BBa_K2066511
component: BBa_K2066515
component: BBa_K2066019
component: BBa_B0015
sequence annotation: BBa_K2066018
sequence annotation: BBa_K864400
sequence annotation: BBa_K2066506
sequence annotation: BBa_K2066515
sequence annotation: BBa_K2066511
sequence annotation: BBa_B0015
sequence annotation: BBa_K2066019
sequence: https://synbiohub.org/public/igem/BBa_K2066025_sequence/1

BBa_K2066018
type: Component
persistent identity: component2530730
display id: component2530730
version: 1
top level: BBa_K2066025
definition: BBa_K2066018
access: public

BBa_K2066506
type: Component
persistent identity: component2530736
display id: component2530736
version: 1
top level: BBa_K2066025
definition: BBa_K2066506
access: public

BBa_K864400
type: Component
persistent identity: component2530735
display id: component2530735
version: 1
top level: BBa_K2066025
definition: BBa_K864400
access: public

BBa_K2066511
type: Component
persistent identity: component2530738
display id: component2530738
version: 1
top level: BBa_K2066025
definition: BBa_K2066511
access: public

BBa_K2066515
type: Component
persistent identity: component2530737
display id: component2530737
version: 1
top level: BBa_K2066025
definition: BBa_K2066515
access: public

BBa_K2066019
type: Component
persistent identity: component2530746
display id: component2530746
version: 1
top level: BBa_K2066025
definition: BBa_K2066019
access: public

BBa_B0015
type: Component
persistent identity: component2530745
display id: component2530745
version: 1
top level: BBa_K2066025
definition: BBa_B0015
access: public

BBa_K2066018
type: SequenceAnnotation
persistent identity: annotation2530730
display id: annotation2530730
version: 1
top level: BBa_K2066025
location: https://synbiohub.org/public/igem/BBa_K2066025/annotation2530730/range2530730/1
component: BBa_K2066018

https://synbiohub.org/public/igem/BBa_K2066025/annotation2530730/range2530730/1
type: Range
persistent identity: range2530730
display id: range2530730
version: 1
top level: BBa_K2066025
direction: direction/reverse
start: 1
end: 40
orientation: inline

BBa_K864400
type: SequenceAnnotation
persistent identity: annotation2530735
display id: annotation2530735
version: 1
top level: BBa_K2066025
location: https://synbiohub.org/public/igem/BBa_K2066025/annotation2530735/range2530735/1
component: BBa_K864400

https://synbiohub.org/public/igem/BBa_K2066025/annotation2530735/range2530735/1
type: Range
persistent identity: range2530735
display id: range2530735
version: 1
top level: BBa_K2066025
direction: direction/reverse
start: 41
end: 101
orientation: inline

BBa_K2066506
type: SequenceAnnotation
persistent identity: annotation2530736
display id: annotation2530736
version: 1
top level: BBa_K2066025
location: https://synbiohub.org/public/igem/BBa_K2066025/annotation2530736/range2530736/1
component: BBa_K2066506

https://synbiohub.org/public/igem/BBa_K2066025/annotation2530736/range2530736/1
type: Range
persistent identity: range2530736
display id: range2530736
version: 1
top level: BBa_K2066025
direction: direction/reverse
start: 102
end: 182
orientation: inline

BBa_K2066515
type: SequenceAnnotation
persistent identity: annotation2530737
display id: annotation2530737
version: 1
top level: BBa_K2066025
location: https://synbiohub.org/public/igem/BBa_K2066025/annotation2530737/range2530737/1
component: BBa_K2066515

https://synbiohub.org/public/igem/BBa_K2066025/annotation2530737/range2530737/1
type: Range
persistent identity: range2530737
display id: range2530737
version: 1
top level: BBa_K2066025
direction: direction/reverse
start: 183
end: 194
orientation: inline

BBa_K2066511
type: SequenceAnnotation
persistent identity: annotation2530738
display id: annotation2530738
version: 1
top level: BBa_K2066025
location: https://synbiohub.org/public/igem/BBa_K2066025/annotation2530738/range2530738/1
component: BBa_K2066511

https://synbiohub.org/public/igem/BBa_K2066025/annotation2530738/range2530738/1
type: Range
persistent identity: range2530738
display id: range2530738
version: 1
top level: BBa_K2066025
direction: direction/reverse
start: 195
end: 1634
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2530745
display id: annotation2530745
version: 1
top level: BBa_K2066025
location: https://synbiohub.org/public/igem/BBa_K2066025/annotation2530745/range2530745/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2066025/annotation2530745/range2530745/1
type: Range
persistent identity: range2530745
display id: range2530745
version: 1
top level: BBa_K2066025
direction: direction/reverse
start: 1635
end: 1763
orientation: inline

BBa_K2066019
type: SequenceAnnotation
persistent identity: annotation2530746
display id: annotation2530746
version: 1
top level: BBa_K2066025
location: https://synbiohub.org/public/igem/BBa_K2066025/annotation2530746/range2530746/1
component: BBa_K2066019

https://synbiohub.org/public/igem/BBa_K2066025/annotation2530746/range2530746/1
type: Range
persistent identity: range2530746
display id: range2530746
version: 1
top level: BBa_K2066025
direction: direction/reverse
start: 1764
end: 1803
orientation: inline

BBa_K2066018
type: ComponentDefinition
persistent identity: BBa K2066018
display id: BBa_K2066018
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 2 Sequence, from Torella et al., 2013
created: 2016-07-11T11:00:00Z
modified: 2016-10-19T05:41:43Z
mutable provenance: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
owned by: james
owned by: myers
mutable description: This is Unique Nucleotide Sequence 2, (UNS 2), from Torella et al., 2013. The William and Mary iGEM team has adopted this as our standard prefix; as such, all of our parts will have this sequence immediately following the BioBrick prefix. We took this measure in order to allow easier Gibson Assembly cloning of our parts. Primer sequences which can be used to clone with the UNS 2/3 standard can be found on our wiki.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: UNS 2 was chosen because it works well with UNS 3 and it is in accordance with the BioBrick standard.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K2066018_sequence/1

BBa_K2066019
type: ComponentDefinition
persistent identity: BBa K2066019
display id: BBa_K2066019
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 3 Sequence, from Torella et al., 2013
created: 2016-07-11T11:00:00Z
modified: 2016-10-19T05:43:00Z
mutable provenance: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
owned by: james
owned by: myers
mutable description: This is Unique Nucleotide Sequence 3, (UNS 3), from Torella et al., 2013. The William and Mary iGEM team has adopted this as our standard prefix; as such, all of our parts will have this sequence immediately following the BioBrick prefix. We took this measure in order to allow easier Gibson Assembly cloning of our parts. Primer sequences which can be used to clone with the UNS 2/3 standard can be found on our wiki. The sequence for this part came from the following paper: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860. A huge thanks to all the researchers involved in its original creation!
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This UNS sequence was chosen to serve as the 3' primer in our standard because it works well with UNS 2 and it adheres to the BioBrick standards.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066019_sequence/1

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K864400
type: ComponentDefinition
persistent identity: BBa K864400
display id: BBa_K864400
version: 1
was generated by: iGEM to SBOL conversion
description: Ptac, trp & lac regulated promoter
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:37Z
mutable provenance: Created from oligos ordered from SigmaAldrich. Oligo sequences: Ptac+ AATTCGCGGCCGCTTCTAGAGGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTA Ptac- CTAGTAAATTGTTATCCGCTCACAATTCCACACATTATACGAGCCGATGATTAATTGTCAACAGCTCCTCTAGAAGCGGCCGCG
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1]. This part also exist together with lacI, part BBa_K180000 [1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25
discontinued: false
dominant: false
experience: experience/Works
group u list: _1124_
m user id: 0
owner id: 9827
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Oligos ordered from SigmaAldrich were annealed to form a double stranded DNA segment, ready to be ligated into any BioBrick backbone.
star: false
creator: Erik Lundin
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Ptac
sequence annotation: -35
sequence annotation: -10
sequence annotation: lacO1 site
sequence: https://synbiohub.org/public/igem/BBa_K864400_sequence/1

Ptac
type: SequenceAnnotation
persistent identity: annotation2197236
display id: annotation2197236
version: 1
top level: BBa_K864400
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197236/range2197236/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K864400/annotation2197236/range2197236/1
type: Range
persistent identity: range2197236
display id: range2197236
version: 1
top level: BBa_K864400
direction: direction/forward
start: 1
end: 40
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2197237
display id: annotation2197237
version: 1
top level: BBa_K864400
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197237/range2197237/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K864400/annotation2197237/range2197237/1
type: Range
persistent identity: range2197237
display id: range2197237
version: 1
top level: BBa_K864400
direction: direction/forward
start: 7
end: 12
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2197238
display id: annotation2197238
version: 1
top level: BBa_K864400
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197238/range2197238/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K864400/annotation2197238/range2197238/1
type: Range
persistent identity: range2197238
display id: range2197238
version: 1
top level: BBa_K864400
direction: direction/forward
start: 29
end: 33
orientation: inline

lacO1 site
type: SequenceAnnotation
persistent identity: annotation2197240
display id: annotation2197240
version: 1
top level: BBa_K864400
location: https://synbiohub.org/public/igem/BBa_K864400/annotation2197240/range2197240/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K864400/annotation2197240/range2197240/1
type: Range
persistent identity: range2197240
display id: range2197240
version: 1
top level: BBa_K864400
direction: direction/forward
start: 36
end: 61
orientation: inline

BBa_K2066506
type: ComponentDefinition
persistent identity: BBa K2066506
display id: BBa_K2066506
version: 1
was generated by: iGEM to SBOL conversion
description: RiboJ (Ribozyme Insulator) Lou et. al. 2012
created: 2016-08-30T11:00:00Z
modified: 2016-10-12T12:27:33Z
mutable provenance: Part sequence is from Lou et al. 2012, Supplemental Section V (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???).
owned by: james
owned by: myers
mutable description: RiboJ is the sequence for a ribozyme studied in Lou et. al 2012 ("Ribozyme-based insulator parts buffer synthetic circuits from genetic context"). WM iGEM 2016 used this sequence between the promoter and ribosome sequence. One of our goals for using this part is moving it onto a Biobrick backbone. Furthermore, In Lou et. al, this ribozyme sequence was said to act as an insulator which generalizes protein expression levels for a given promoter. We used RiboJ to collect data for our Ribozyme characterization project as well as our ribosome and promoter characterization projects.
discontinued: false
dominant: false
group u list: _2534_
m user id: 27446
owner id: 31541
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We designed this part to use as an insulator and also move this riboJ sequence onto a Biobrick backbone.
star: false
creator: Likhitha Kolla
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K2066506_sequence/1

BBa_K2066511
type: ComponentDefinition
persistent identity: BBa K2066511
display id: BBa_K2066511
version: 1
was generated by: iGEM to SBOL conversion
description: cI-GFP
created: 2016-08-31T11:00:00Z
modified: 2016-09-01T05:55:35Z
mutable provenance: The sequence is modified from Lou et al. Supplement section V "Ribozyme-based insulator parts buffer synthetic circuits from genetic context".
owned by: james
owned by: myers
mutable description: cI-GFP is used for our Ribozyme Characterization project. It is a reporter molecule fused with cI used to look at the input-output transfer functions of IPTG-cIGFP both with the RiboJ insulator and without (Lou et al 2012). The sequence is modified from Lou et al. Supplement section V. However, within this sequence the T at position 1161 used to be a G; the T at 1166 used to be a C; the C at 1215 used to be a T; the A at 1282 used to be a C. All single-nucleotide substitutions were designed to preserve amino acid sequence and result in a <1% change in codon usage frequency in E. coli.)
discontinued: false
dominant: false
group u list: _2534_
m user id: 31541
owner id: 31541
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We wanted this part for our ribozyme characterization project.
star: false
creator: Likhitha Kolla
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K2066511_sequence/1

BBa_K2066515
type: ComponentDefinition
persistent identity: BBa K2066515
display id: BBa_K2066515
version: 1
was generated by: iGEM to SBOL conversion
description: RBS2 (weak)
created: 2016-10-06T11:00:00Z
modified: 2016-10-07T01:11:58Z
mutable provenance: Supplement section V in Lou et al. 2012 (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???)
owned by: james
owned by: myers
mutable description: This is a weak RBS from Lou et al. 2012 (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???) used in the construction of some pTAC Ribozyme Characterization Parts.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 31544
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_K2066515_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K2066025_sequence/1
type: Sequence
persistent identity: BBa K2066025 sequence
display id: BBa_K2066025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgggagttcgtagacggaaacaaacgcagaatccaagcgagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaattagctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaaactagaaccatgaaaaaagtgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcggcggtggcggtcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtgtacattaccgcagataaacaaaaaaatggcattaaagcgaatttcaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagcactgaaggtcctcaatcgcactggaaacatcaaggtcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066018_sequence/1
type: Sequence
persistent identity: BBa K2066018 sequence
display id: BBa_K2066018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgggagttcgtagacggaaacaaacgcagaatccaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066019_sequence/1
type: Sequence
persistent identity: BBa K2066019 sequence
display id: BBa_K2066019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcactgaaggtcctcaatcgcactggaaacatcaaggtcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K864400_sequence/1
type: Sequence
persistent identity: BBa K864400 sequence
display id: BBa_K864400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagctgttgacaattaatcatcggctcgtataatgtgtggaattgtgagcggataacaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066506_sequence/1
type: Sequence
persistent identity: BBa K2066506 sequence
display id: BBa_K2066506_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaaactaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066511_sequence/1
type: Sequence
persistent identity: BBa K2066511 sequence
display id: BBa_K2066511_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcggcggtggcggtcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtgtacattaccgcagataaacaaaaaaatggcattaaagcgaatttcaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066515_sequence/1
type: Sequence
persistent identity: BBa K2066515 sequence
display id: BBa_K2066515_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accatgaaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z