LOCUS       BBa_K2066028            3235 bp    DNA     linear       17-May-2024
DEFINITION  pBAD - LacI/Mcherry fusion
ACCESSION   BBa_K2066028
VERSION     BBa_K2066028.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     promoter        1120..1250
                     /label=Pbad
     promoter        1083..1154
                     /label=PC
     misc_feature    41..1119
                     /label=AraC
     promoter        41..1250
                     /label=BBa_I0500
     misc_feature    1255..1258
                     /label=conserved
     RBS             1251..1262
                     /label=BBa_B0034
     CDS             1263..2354
                     /label=BBa_K2066512
     CDS             2355..3066
                     /label=BBa_K2066513
     stem_loop       3078..3121
                     /label=stem_loop
     misc_feature    3067..3146
                     /label=BBa_B0010
     terminator      3067..3146
                     /label=BBa_B0010
     stem_loop       3162..3181
                     /label=T7 TE
     misc_feature    3188..3188
                     /label=stop
     polyA_site      3182..3195
                     /label=polya
     misc_feature    3155..3195
                     /label=BBa_B0012
     terminator      3155..3195
                     /label=BBa_B0012
     terminator      3067..3195
                     /label=BBa_B0015
     misc_feature    3196..3235
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc ttatgacaac ttgacggcta
       61 catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt
      121 ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg
      181 cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc
      241 gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg
      301 acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc
      361 tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa
      421 tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat
      481 agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct
      541 ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc
      601 attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct
      661 ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc
      721 ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga
      781 gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct
      841 caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat
      901 cattttgcgc ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc
      961 catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac
     1021 cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa
     1081 cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg
     1141 tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct
     1201 ttttatcgca actctctact gtttctccat acccgttttt ttgggctagc aaagaggaga
     1261 aagtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg
     1321 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag
     1381 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac
     1441 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg
     1501 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag
     1561 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca
     1621 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct
     1681 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta
     1741 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc
     1801 agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg
     1861 gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact
     1921 ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca
     1981 ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt
     2041 ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct
     2101 catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca
     2161 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc
     2221 ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc
     2281 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2341 agggtggttc tggtgtgagc aagggcgaag aagataacat ggccatcatc aaggagttca
     2401 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     2461 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     2521 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     2581 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     2641 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     2701 cccttgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     2761 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     2821 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     2881 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     2941 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     3001 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     3061 taataaccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     3121 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     3181 ctttctgcgt ttatagcact gaaggtcctc aatcgcactg gaaacatcaa ggtcg
//