LOCUS       BBa_K2066029            2775 bp    DNA     linear       17-May-2024
DEFINITION  pBad - TetR/mCherry Fusion
ACCESSION   BBa_K2066029
VERSION     BBa_K2066029.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     promoter        1120..1250
                     /label=Pbad
     promoter        1083..1154
                     /label=PC
     misc_feature    41..1119
                     /label=AraC
     promoter        41..1250
                     /label=BBa_I0500
     misc_feature    1255..1258
                     /label=conserved
     RBS             1251..1262
                     /label=BBa_B0034
     CDS             1263..1895
                     /label=BBa_K2066516
     CDS             1896..2606
                     /label=BBa_K2066513
     stem_loop       2618..2661
                     /label=stem_loop
     misc_feature    2607..2686
                     /label=BBa_B0010
     terminator      2607..2686
                     /label=BBa_B0010
     stem_loop       2702..2721
                     /label=T7 TE
     misc_feature    2728..2728
                     /label=stop
     polyA_site      2722..2735
                     /label=polya
     misc_feature    2695..2735
                     /label=BBa_B0012
     terminator      2695..2735
                     /label=BBa_B0012
     terminator      2607..2735
                     /label=BBa_B0015
     misc_feature    2736..2775
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc ttatgacaac ttgacggcta
       61 catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt
      121 ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg
      181 cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc
      241 gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg
      301 acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc
      361 tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa
      421 tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat
      481 agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct
      541 ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc
      601 attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct
      661 ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc
      721 ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga
      781 gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct
      841 caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat
      901 cattttgcgc ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc
      961 catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac
     1021 cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa
     1081 cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg
     1141 tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct
     1201 ttttatcgca actctctact gtttctccat acccgttttt ttgggctagc aaagaggaga
     1261 aaatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
     1321 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
     1381 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
     1441 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
     1501 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
     1561 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
     1621 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
     1681 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
     1741 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
     1801 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
     1861 aacttaaatg tgaaagtggg tctggtggtt ctggtgtgag caagggcgaa gaagataaca
     1921 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     1981 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     2041 agctgaaggt gaccaagggt ggccccctgc cttcgcctgg gacatcctgt cccctcagtt
     2101 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     2161 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     2221 gaccgtgacc caggactcct cccttgcagg acggcgagtt catctacaag gtgaagctgc
     2281 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
     2341 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     2401 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     2461 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     2521 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     2581 gcatggacga gctgtacaag taataaccag gcatcaaata aaacgaaagg ctcagtcgaa
     2641 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     2701 tggctcacct tcgggtgggc ctttctgcgt ttatagcact gaaggtcctc aatcgcactg
     2761 gaaacatcaa ggtcg
//