LOCUS       BBa_K2066033            2201 bp    DNA     linear       17-May-2024
DEFINITION  pBad - RFP on UNS
ACCESSION   BBa_K2066033
VERSION     BBa_K2066033.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     misc_feature    41..1154
                     /label=BBa_I13458
     promoter        1163..1292
                     /label=BBa_I13453
     misc_feature    1305..1308
                     /label=conserved
     RBS             1301..1312
                     /label=BBa_B0034
     CDS             1319..1993
                     /label=mrfp1
     misc_feature    2000..2024
                     /label=Help:Barcodes
     CDS             1319..2024
                     /label=BBa_E1010
     stem_loop       2044..2087
                     /label=stem_loop
     misc_feature    2033..2112
                     /label=BBa_B0010
     terminator      2033..2112
                     /label=BBa_B0010
     stem_loop       2128..2147
                     /label=T7 TE
     misc_feature    2154..2154
                     /label=stop
     polyA_site      2148..2161
                     /label=polya
     misc_feature    2121..2161
                     /label=BBa_B0012
     terminator      2121..2161
                     /label=BBa_B0012
     terminator      2033..2161
                     /label=BBa_B0015
     misc_feature    41..2161
                     /label=BBa_K577882
     misc_feature    2162..2201
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc ttatgacaac ttgacggcta
       61 catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt
      121 ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg
      181 cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc
      241 gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg
      301 acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc
      361 tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa
      421 tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat
      481 agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct
      541 ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc
      601 attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct
      661 ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc
      721 ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga
      781 gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct
      841 caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat
      901 cattttgcgc ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc
      961 catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac
     1021 cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa
     1081 cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg
     1141 tcacactttg ctattactag agacattgat tatttgcacg gcgtcacact ttgctatgcc
     1201 atagcatttt tatccataag attagcggat cctacctgac gctttttatc gcaactctct
     1261 actgtttctc cataccgttt ttttgggcta gctactagag aaagaggaga aatactagat
     1321 ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc
     1381 cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac
     1441 ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct
     1501 gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga
     1561 ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga
     1621 cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa
     1681 agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat
     1741 gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat
     1801 caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta
     1861 catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat
     1921 cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca
     1981 ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat
     2041 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     2101 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     2161 agcactgaag gtcctcaatc gcactggaaa catcaaggtc g
//