BBa_K2066039
type: ComponentDefinition
persistent identity: BBa K2066039
display id: BBa_K2066039
version: 1
was generated by: iGEM to SBOL conversion
description: Sigma54 Enhancer 57S with DT, UNS Standard
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T09:08:36Z
mutable provenance: to be completed
owned by: james
owned by: myers
mutable description: to be completed
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: to be completed
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K2066543
component: BBa_K2066018
component: BBa_K2066518
component: BBa_K2066546
component: BBa_K2066019
component: BBa_B0015
component: BBa_K2066544
component: BBa_K2066548
component: BBa_K2066547
component: BBa_K2066545
sequence annotation: BBa_K2066018
sequence annotation: BBa_K2066543
sequence annotation: BBa_K2066548
sequence annotation: BBa_K2066544
sequence annotation: BBa_K2066545
sequence annotation: BBa_K2066547
sequence annotation: BBa_K2066546
sequence annotation: BBa_K2066518
sequence annotation: BBa_B0015
sequence annotation: BBa_K2066019
sequence: https://synbiohub.org/public/igem/BBa_K2066039_sequence/1

BBa_K2066543
type: Component
persistent identity: component2524227
display id: component2524227
version: 1
top level: BBa_K2066039
definition: BBa_K2066543
access: public

BBa_K2066018
type: Component
persistent identity: component2524226
display id: component2524226
version: 1
top level: BBa_K2066039
definition: BBa_K2066018
access: public

BBa_K2066518
type: Component
persistent identity: component2524233
display id: component2524233
version: 1
top level: BBa_K2066039
definition: BBa_K2066518
access: public

BBa_K2066546
type: Component
persistent identity: component2524232
display id: component2524232
version: 1
top level: BBa_K2066039
definition: BBa_K2066546
access: public

BBa_K2066019
type: Component
persistent identity: component2524241
display id: component2524241
version: 1
top level: BBa_K2066039
definition: BBa_K2066019
access: public

BBa_B0015
type: Component
persistent identity: component2524240
display id: component2524240
version: 1
top level: BBa_K2066039
definition: BBa_B0015
access: public

BBa_K2066544
type: Component
persistent identity: component2524229
display id: component2524229
version: 1
top level: BBa_K2066039
definition: BBa_K2066544
access: public

BBa_K2066548
type: Component
persistent identity: component2524228
display id: component2524228
version: 1
top level: BBa_K2066039
definition: BBa_K2066548
access: public

BBa_K2066547
type: Component
persistent identity: component2524231
display id: component2524231
version: 1
top level: BBa_K2066039
definition: BBa_K2066547
access: public

BBa_K2066545
type: Component
persistent identity: component2524230
display id: component2524230
version: 1
top level: BBa_K2066039
definition: BBa_K2066545
access: public

BBa_K2066018
type: SequenceAnnotation
persistent identity: annotation2524226
display id: annotation2524226
version: 1
top level: BBa_K2066039
location: https://synbiohub.org/public/igem/BBa_K2066039/annotation2524226/range2524226/1
component: BBa_K2066018

https://synbiohub.org/public/igem/BBa_K2066039/annotation2524226/range2524226/1
type: Range
persistent identity: range2524226
display id: range2524226
version: 1
top level: BBa_K2066039
direction: direction/reverse
start: 1
end: 40
orientation: inline

BBa_K2066543
type: SequenceAnnotation
persistent identity: annotation2524227
display id: annotation2524227
version: 1
top level: BBa_K2066039
location: https://synbiohub.org/public/igem/BBa_K2066039/annotation2524227/range2524227/1
component: BBa_K2066543

https://synbiohub.org/public/igem/BBa_K2066039/annotation2524227/range2524227/1
type: Range
persistent identity: range2524227
display id: range2524227
version: 1
top level: BBa_K2066039
direction: direction/reverse
start: 41
end: 87
orientation: inline

BBa_K2066548
type: SequenceAnnotation
persistent identity: annotation2524228
display id: annotation2524228
version: 1
top level: BBa_K2066039
location: https://synbiohub.org/public/igem/BBa_K2066039/annotation2524228/range2524228/1
component: BBa_K2066548

https://synbiohub.org/public/igem/BBa_K2066039/annotation2524228/range2524228/1
type: Range
persistent identity: range2524228
display id: range2524228
version: 1
top level: BBa_K2066039
direction: direction/reverse
start: 88
end: 236
orientation: inline

BBa_K2066544
type: SequenceAnnotation
persistent identity: annotation2524229
display id: annotation2524229
version: 1
top level: BBa_K2066039
location: https://synbiohub.org/public/igem/BBa_K2066039/annotation2524229/range2524229/1
component: BBa_K2066544

https://synbiohub.org/public/igem/BBa_K2066039/annotation2524229/range2524229/1
type: Range
persistent identity: range2524229
display id: range2524229
version: 1
top level: BBa_K2066039
direction: direction/reverse
start: 237
end: 266
orientation: inline

BBa_K2066545
type: SequenceAnnotation
persistent identity: annotation2524230
display id: annotation2524230
version: 1
top level: BBa_K2066039
location: https://synbiohub.org/public/igem/BBa_K2066039/annotation2524230/range2524230/1
component: BBa_K2066545

https://synbiohub.org/public/igem/BBa_K2066039/annotation2524230/range2524230/1
type: Range
persistent identity: range2524230
display id: range2524230
version: 1
top level: BBa_K2066039
direction: direction/reverse
start: 267
end: 288
orientation: inline

BBa_K2066547
type: SequenceAnnotation
persistent identity: annotation2524231
display id: annotation2524231
version: 1
top level: BBa_K2066039
location: https://synbiohub.org/public/igem/BBa_K2066039/annotation2524231/range2524231/1
component: BBa_K2066547

https://synbiohub.org/public/igem/BBa_K2066039/annotation2524231/range2524231/1
type: Range
persistent identity: range2524231
display id: range2524231
version: 1
top level: BBa_K2066039
direction: direction/reverse
start: 289
end: 431
orientation: inline

BBa_K2066546
type: SequenceAnnotation
persistent identity: annotation2524232
display id: annotation2524232
version: 1
top level: BBa_K2066039
location: https://synbiohub.org/public/igem/BBa_K2066039/annotation2524232/range2524232/1
component: BBa_K2066546

https://synbiohub.org/public/igem/BBa_K2066039/annotation2524232/range2524232/1
type: Range
persistent identity: range2524232
display id: range2524232
version: 1
top level: BBa_K2066039
direction: direction/reverse
start: 433
end: 1926
orientation: inline

BBa_K2066518
type: SequenceAnnotation
persistent identity: annotation2524233
display id: annotation2524233
version: 1
top level: BBa_K2066039
location: https://synbiohub.org/public/igem/BBa_K2066039/annotation2524233/range2524233/1
component: BBa_K2066518

https://synbiohub.org/public/igem/BBa_K2066039/annotation2524233/range2524233/1
type: Range
persistent identity: range2524233
display id: range2524233
version: 1
top level: BBa_K2066039
direction: direction/reverse
start: 1927
end: 2640
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2524240
display id: annotation2524240
version: 1
top level: BBa_K2066039
location: https://synbiohub.org/public/igem/BBa_K2066039/annotation2524240/range2524240/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2066039/annotation2524240/range2524240/1
type: Range
persistent identity: range2524240
display id: range2524240
version: 1
top level: BBa_K2066039
direction: direction/reverse
start: 2641
end: 2769
orientation: inline

BBa_K2066019
type: SequenceAnnotation
persistent identity: annotation2524241
display id: annotation2524241
version: 1
top level: BBa_K2066039
location: https://synbiohub.org/public/igem/BBa_K2066039/annotation2524241/range2524241/1
component: BBa_K2066019

https://synbiohub.org/public/igem/BBa_K2066039/annotation2524241/range2524241/1
type: Range
persistent identity: range2524241
display id: range2524241
version: 1
top level: BBa_K2066039
direction: direction/reverse
start: 2770
end: 2809
orientation: inline

BBa_K2066018
type: ComponentDefinition
persistent identity: BBa K2066018
display id: BBa_K2066018
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 2 Sequence, from Torella et al., 2013
created: 2016-07-11T11:00:00Z
modified: 2016-10-19T05:41:43Z
mutable provenance: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
owned by: james
owned by: myers
mutable description: This is Unique Nucleotide Sequence 2, (UNS 2), from Torella et al., 2013. The William and Mary iGEM team has adopted this as our standard prefix; as such, all of our parts will have this sequence immediately following the BioBrick prefix. We took this measure in order to allow easier Gibson Assembly cloning of our parts. Primer sequences which can be used to clone with the UNS 2/3 standard can be found on our wiki.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: UNS 2 was chosen because it works well with UNS 3 and it is in accordance with the BioBrick standard.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K2066018_sequence/1

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K2066518
type: ComponentDefinition
persistent identity: BBa K2066518
display id: BBa_K2066518
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry (with start codon)
created: 2016-10-07T11:00:00Z
modified: 2016-10-08T10:50:28Z
owned by: james
owned by: myers
mutable description: Modified from the mCherry used in K2066028 and K2066029 to include the start codon. This modified mCherry is used in the construction of K2066040 and K2066041.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 31544
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The start codon was removed in K2066028 and K2066029 to follow the design from Synthetic Analog Computation in Living Cells (Daniel et al.) where the codon bias was being optimized.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K2066518_sequence/1

BBa_K2066543
type: ComponentDefinition
persistent identity: BBa K2066543
display id: BBa_K2066543
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: Enhancer/NRI binding sites
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T04:20:03Z
mutable provenance: mep
owned by: james
owned by: myers
mutable description: tmept
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: temp
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K2066543_sequence/1

BBa_K2066544
type: ComponentDefinition
persistent identity: BBa K2066544
display id: BBa_K2066544
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: glnAp2 promoter
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T04:48:44Z
mutable provenance: temp
owned by: james
owned by: myers
mutable description: temp
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: temp
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K2066544_sequence/1

BBa_K2066545
type: ComponentDefinition
persistent identity: BBa K2066545
display id: BBa_K2066545
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: LacI binding site (LacO)
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:05:36Z
mutable provenance: temp
owned by: james
owned by: myers
mutable description: temp
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: tem
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K2066545_sequence/1

BBa_K2066546
type: ComponentDefinition
persistent identity: BBa K2066546
display id: BBa_K2066546
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: glnG (NRI)
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:07:26Z
mutable provenance: mept
owned by: james
owned by: myers
mutable description: tempt
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: metp
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2066546_sequence/1

BBa_K2066547
type: ComponentDefinition
persistent identity: BBa K2066547
display id: BBa_K2066547
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: Spacer between Promoter and RBS
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:11:55Z
mutable provenance: petm
owned by: james
owned by: myers
mutable description: ptem
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: ep
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066547_sequence/1

BBa_K2066548
type: ComponentDefinition
persistent identity: BBa K2066548
display id: BBa_K2066548
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: 57S (2 Tet binding cassette)
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:18:11Z
mutable provenance: ptem
owned by: james
owned by: myers
mutable description: ptem
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: ep
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066548_sequence/1

BBa_K2066019
type: ComponentDefinition
persistent identity: BBa K2066019
display id: BBa_K2066019
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 3 Sequence, from Torella et al., 2013
created: 2016-07-11T11:00:00Z
modified: 2016-10-19T05:43:00Z
mutable provenance: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
owned by: james
owned by: myers
mutable description: This is Unique Nucleotide Sequence 3, (UNS 3), from Torella et al., 2013. The William and Mary iGEM team has adopted this as our standard prefix; as such, all of our parts will have this sequence immediately following the BioBrick prefix. We took this measure in order to allow easier Gibson Assembly cloning of our parts. Primer sequences which can be used to clone with the UNS 2/3 standard can be found on our wiki. The sequence for this part came from the following paper: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860. A huge thanks to all the researchers involved in its original creation!
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This UNS sequence was chosen to serve as the 3' primer in our standard because it works well with UNS 2 and it adheres to the BioBrick standards.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066019_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K2066039_sequence/1
type: Sequence
persistent identity: BBa K2066039 sequence
display id: BBa_K2066039_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgggagttcgtagacggaaacaaacgcagaatccaagctcacaattgcaccaacatggtgcttaatgtttccattgaagcactatattggtgcaacattgtcttcgacgctagctccctatcagtgatagagattgggtccggccagactcgagctttgcgccaccgtctccgcgcgctgcgcgcggatcgcctgcacggagcgctagcgtccccatgataacgccttttaggggcaatttaaaagttggcacagatttcgctttatcttttttaattgtgagcgctcacaattaagcttggcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataaacgtttatgcaacgagggatagtctgggtagtcgatgacgatagttccatccgttgggtgcttgaacgtgcgctcgctggggcaggtttaacctgtacgacgtttgagaacggcgcagaagtgctggaggcgctggcgagcaaaacgccggatgtgctgctttcagatatccgtatgccgggaatggacgggctggcgctgctcaagcagattaaacagcgccatccaatgcttccggtcatcattatgaccgcacattccgatctggatgctgccgtcagcgcctatcaacaaggggcgtttgattatctgcccaaaccgtttgatatcgacgaagcagtggcgctggttgagcgcgctatcagtcattaccaggaacagcagcagccgcgtaatgttcagcttaacggcccaacgaccgatatcatcggcgaagcgccagccatgcaggacgtgttccgtattatcggtcggctttcgcgttcttctattagcgtgctgattaacggcgaatccggcaccggtaaagaactggtcgctcatgccctgcatcgccacagtccgcgcgccaaagcgccgtttatcgcgctgaatatggcagctatcccaaaagatttgatcgaatcagaactgtttggccacgagaaaggcgcgtttactggcgcgaataccattcgtcaggggcgttttgaacaggccgatggcggtacattattcctcgacgaaattggtgatatgccgctggatgtgcagacgcgtttgctgcgcgtgctggcagacggtcagttttaccgcgttggcggctatgcgccggtgaaagtggatgtgcggattatcgctgccactcaccagaatctcgaacagcgagtgcaggaaggtaagttccgtgaggatctgttccaccgcctgaacgttatccgcgttcatctgccgccgctgcgcgaacgtcgggaagatattccccgtctggcgcgccattttttacaggttgccgcgcgcgaactgggcgtagaagcgaagttactgcatccggaaaccgaagctgctctgacgcgtctggcgtggccaggcaacgtgcgccagctggaaaacacctgccgctggctaacggtgatggccgccgggcaggaagtgttgattcaggatttgcccggcgaactgtttgaatcaacggttgcggagagtacttcgcaaatgcaaccggacagctgggcgacgcttcttgcgcagtgggcagacagagcgctgcgttccggtcatcaaaatctgctttccgaagcgcagccagagctggagcggacgttactgacgaccgcgttgcgacatacgcaggggcataaacaggaagcggcgcggctactcggctggggccgcaacaccctgacgcgtaagttaaaagagctggggatggagtgattcacagcttgtgtgtaaagattgattattgagcgcaaattgctggtattttacgctttactgttccaggaggaaaaaaatggtgagcaagggcgaagaagataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggaggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066018_sequence/1
type: Sequence
persistent identity: BBa K2066018 sequence
display id: BBa_K2066018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgggagttcgtagacggaaacaaacgcagaatccaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066518_sequence/1
type: Sequence
persistent identity: BBa K2066518 sequence
display id: BBa_K2066518_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaagaagataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066543_sequence/1
type: Sequence
persistent identity: BBa K2066543 sequence
display id: BBa_K2066543_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacaattgcaccaacatggtgcttaatgtttccattgaagcactat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066544_sequence/1
type: Sequence
persistent identity: BBa K2066544 sequence
display id: BBa_K2066544_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaatttaaaagttggcacagatttcgctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066545_sequence/1
type: Sequence
persistent identity: BBa K2066545 sequence
display id: BBa_K2066545_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tatcttttttaattgtgagcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066546_sequence/1
type: Sequence
persistent identity: BBa K2066546 sequence
display id: BBa_K2066546_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtttatgcaacgagggatagtctgggtagtcgatgacgatagttccatccgttgggtgcttgaacgtgcgctcgctggggcaggtttaacctgtacgacgtttgagaacggcgcagaagtgctggaggcgctggcgagcaaaacgccggatgtgctgctttcagatatccgtatgccgggaatggacgggctggcgctgctcaagcagattaaacagcgccatccaatgcttccggtcatcattatgaccgcacattccgatctggatgctgccgtcagcgcctatcaacaaggggcgtttgattatctgcccaaaccgtttgatatcgacgaagcagtggcgctggttgagcgcgctatcagtcattaccaggaacagcagcagccgcgtaatgttcagcttaacggcccaacgaccgatatcatcggcgaagcgccagccatgcaggacgtgttccgtattatcggtcggctttcgcgttcttctattagcgtgctgattaacggcgaatccggcaccggtaaagaactggtcgctcatgccctgcatcgccacagtccgcgcgccaaagcgccgtttatcgcgctgaatatggcagctatcccaaaagatttgatcgaatcagaactgtttggccacgagaaaggcgcgtttactggcgcgaataccattcgtcaggggcgttttgaacaggccgatggcggtacattattcctcgacgaaattggtgatatgccgctggatgtgcagacgcgtttgctgcgcgtgctggcagacggtcagttttaccgcgttggcggctatgcgccggtgaaagtggatgtgcggattatcgctgccactcaccagaatctcgaacagcgagtgcaggaaggtaagttccgtgaggatctgttccaccgcctgaacgttatccgcgttcatctgccgccgctgcgcgaacgtcgggaagatattccccgtctggcgcgccattttttacaggttgccgcgcgcgaactgggcgtagaagcgaagttactgcatccggaaaccgaagctgctctgacgcgtctggcgtggccaggcaacgtgcgccagctggaaaacacctgccgctggctaacggtgatggccgccgggcaggaagtgttgattcaggatttgcccggcgaactgtttgaatcaacggttgcggagagtacttcgcaaatgcaaccggacagctgggcgacgcttcttgcgcagtgggcagacagagcgctgcgttccggtcatcaaaatctgctttccgaagcgcagccagagctggagcggacgttactgacgaccgcgttgcgacatacgcaggggcataaacaggaagcggcgcggctactcggctggggccgcaacaccctgacgcgtaagttaaaagagctggggatggagtgattcacagcttgtgtgtaaagattgattattgagcgcaaattgctggtattttacgctttactgttccaggaggaaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066547_sequence/1
type: Sequence
persistent identity: BBa K2066547 sequence
display id: BBa_K2066547_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacaattaagcttggcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066548_sequence/1
type: Sequence
persistent identity: BBa K2066548 sequence
display id: BBa_K2066548_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attggtgcaacattgtcttcgacgctagctccctatcagtgatagagattgggtccggccagactcgagctttgcgccaccgtctccgcgcgctgcgcgcggatcgcctgcacggagcgctagcgtccccatgataacgccttttaggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066019_sequence/1
type: Sequence
persistent identity: BBa K2066019 sequence
display id: BBa_K2066019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcactgaaggtcctcaatcgcactggaaacatcaaggtcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z