LOCUS       BBa_K2066040            1355 bp    DNA     linear       22-Nov-2024
DEFINITION  J23100 + RiboJ Promoter Characterization Part with 2x Broccoli
ACCESSION   BBa_K2066040
VERSION     BBa_K2066040.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     promoter        41..75
                     /label=BBa_J23100
     misc_feature    76..150
                     /label=BBa_K2066535
     RBS             151..168
                     /label=BBa_K2066527
     misc_feature    169..882
                     /label=BBa_K2066518
     misc_feature    883..912
                     /label=BBa_K2066519
     misc_feature    913..932
                     /label=BBa_K2066507
     misc_feature    933..1166
                     /label=BBa_K2066520
     misc_feature    1167..1186
                     /label=BBa_K2066521
     stem_loop       1198..1241
                     /label=stem_loop
     misc_feature    1187..1266
                     /label=BBa_B0010
     terminator      1187..1266
                     /label=BBa_B0010
     stem_loop       1282..1301
                     /label=T7 TE
     misc_feature    1308..1308
                     /label=stop
     polyA_site      1302..1315
                     /label=polya
     misc_feature    1275..1315
                     /label=BBa_B0012
     terminator      1275..1315
                     /label=BBa_B0012
     terminator      1187..1315
                     /label=BBa_B0015
     misc_feature    1316..1355
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc ttgacggcta gctcagtcct
       61 aggtacagtg ctagcagctg tcaccggatg tgctttccgg tctgatgagt ccgtgaggac
      121 gaaacagcct ctacaaataa ttttgtttaa aaagaggaga aatactagat ggtgagcaag
      181 ggcgaagaag ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
      241 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      301 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
      361 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
      421 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      481 gaggacggcg gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc
      541 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
      601 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
      661 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
      721 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
      781 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
      841 cgccactcca ccggcggcat ggacgagctg tacaagtaat aactctacga caacctcttc
      901 acagccaatc tcctcgttcg ctgccaccta agttgccatg tgtatgtggg agacggtcgg
      961 gtccatctga gacggtcggg tccagatatt cgtatctgtc gagtagagtg tgggctcaga
     1021 tgtcgagtag agtgtgggct cccacatact ctgatgatcc agacggtcgg gtccatctga
     1081 gacggtcggg tccagatatt cgtatctgtc gagtagagtg tgggctcaga tgtcgagtag
     1141 agtgtgggct ggatcattca tggcaaaata ctctacggtc acatacccag gcatcaaata
     1201 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1261 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatagcact
     1321 gaaggtcctc aatcgcactg gaaacatcaa ggtcg
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