LOCUS       BBa_K2066053            1920 bp    DNA     linear       17-May-2024
DEFINITION  pTET GFP + TetR on UNS
ACCESSION   BBa_K2066053
VERSION     BBa_K2066053.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     misc_feature    41..59
                     /label=TetR 1
     misc_feature    41..94
                     /label=BBa_R0040
     promoter        60..65
                     /label=-35
     misc_feature    66..84
                     /label=TetR 2
     promoter        83..88
                     /label=-10
     promoter        41..94
                     /label=BBa_R0040
     misc_feature    95..102
                     /label=BBa_K2066529
     misc_feature    107..110
                     /label=conserved
     RBS             103..114
                     /label=BBa_B0034
     misc_feature    115..120
                     /label=BBa_K2066530
     CDS             121..840
                     /label=GFP protein
     CDS             121..840
                     /label=BBa_E0040
     misc_feature    841..848
                     /label=BBa_K2066529
     stem_loop       860..903
                     /label=stem_loop
     misc_feature    849..928
                     /label=BBa_B0010
     terminator      849..928
                     /label=BBa_B0010
     stem_loop       944..963
                     /label=T7 TE
     misc_feature    970..970
                     /label=stop
     polyA_site      964..977
                     /label=polya
     misc_feature    937..977
                     /label=BBa_B0012
     terminator      937..977
                     /label=BBa_B0012
     terminator      849..977
                     /label=BBa_B0015
     misc_feature    978..997
                     /label=BBa_K2066507
     promoter        998..1032
                     /label=BBa_J23105
     misc_feature    1033..1040
                     /label=BBa_K2066529
     misc_feature    1045..1048
                     /label=conserved
     RBS             1041..1052
                     /label=BBa_B0034
     misc_feature    1053..1058
                     /label=BBa_K2066530
     CDS             1062..1678
                     /label=tetR
     misc_feature    1679..1712
                     /label=SsrA
     misc_feature    1719..1743
                     /label=Help:Barcodes
     CDS             1059..1743
                     /label=BBa_C0040
     misc_feature    1744..1751
                     /label=BBa_K2066529
     stem_loop       1763..1806
                     /label=stem_loop
     misc_feature    1752..1831
                     /label=BBa_B0010
     terminator      1752..1831
                     /label=BBa_B0010
     stem_loop       1847..1866
                     /label=T7 TE
     misc_feature    1873..1873
                     /label=stop
     polyA_site      1867..1880
                     /label=polya
     misc_feature    1840..1880
                     /label=BBa_B0012
     terminator      1840..1880
                     /label=BBa_B0012
     terminator      1752..1880
                     /label=BBa_B0015
     misc_feature    1881..1920
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc tccctatcag tgatagagat
       61 tgacatccct atcagtgata gagatactga gcactactag agaaagagga gaaatactag
      121 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      181 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      241 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      301 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      361 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      421 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      481 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      541 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      601 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      661 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      721 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      781 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
      841 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      901 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      961 gcctttctgc gtttatactc gttcgctgcc acctaagttt acggctagct cagtcctagg
     1021 tactatgcta gctactagag aaagaggaga aatactagat gtccagatta gataaaagta
     1081 aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc
     1141 gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata
     1201 agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt
     1261 gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat
     1321 gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa
     1381 aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag
     1441 agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag
     1501 atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc
     1561 cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat
     1621 tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg
     1681 ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat
     1741 cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1801 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     1861 tgggcctttc tgcgtttata gcactgaagg tcctcaatcg cactggaaac atcaaggtcg
//