BBa_K2066055
type: ComponentDefinition
persistent identity: BBa K2066055
display id: BBa_K2066055
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Promoter without RiboJ Promoter Characterization Part
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T08:22:26Z
mutable provenance: to be completed
owned by: james
owned by: myers
mutable description: to be completed
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: to be completed
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K2066019
component: BBa_K2066021
component: BBa_K2066520
component: BBa_B0012
component: BBa_B0010
component: BBa_K2066518
component: BBa_K2066527
component: BBa_K2066507
component: BBa_K2066519
component: BBa_K2066537
component: BBa_K2066018
sequence annotation: BBa_K2066018
sequence annotation: BBa_K2066537
sequence annotation: BBa_K2066527
sequence annotation: BBa_K2066518
sequence annotation: BBa_K2066519
sequence annotation: BBa_K2066507
sequence annotation: BBa_K2066520
sequence annotation: BBa_K2066021
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K2066019
sequence: https://synbiohub.org/public/igem/BBa_K2066055_sequence/1

BBa_K2066019
type: Component
persistent identity: component2523926
display id: component2523926
version: 1
top level: BBa_K2066055
definition: BBa_K2066019
access: public

BBa_K2066021
type: Component
persistent identity: component2523919
display id: component2523919
version: 1
top level: BBa_K2066055
definition: BBa_K2066021
access: public

BBa_K2066520
type: Component
persistent identity: component2523918
display id: component2523918
version: 1
top level: BBa_K2066055
definition: BBa_K2066520
access: public

BBa_B0012
type: Component
persistent identity: component2523922
display id: component2523922
version: 1
top level: BBa_K2066055
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2523920
display id: component2523920
version: 1
top level: BBa_K2066055
definition: BBa_B0010
access: public

BBa_K2066518
type: Component
persistent identity: component2523915
display id: component2523915
version: 1
top level: BBa_K2066055
definition: BBa_K2066518
access: public

BBa_K2066527
type: Component
persistent identity: component2523914
display id: component2523914
version: 1
top level: BBa_K2066055
definition: BBa_K2066527
access: public

BBa_K2066507
type: Component
persistent identity: component2523917
display id: component2523917
version: 1
top level: BBa_K2066055
definition: BBa_K2066507
access: public

BBa_K2066519
type: Component
persistent identity: component2523916
display id: component2523916
version: 1
top level: BBa_K2066055
definition: BBa_K2066519
access: public

BBa_K2066537
type: Component
persistent identity: component2523913
display id: component2523913
version: 1
top level: BBa_K2066055
definition: BBa_K2066537
access: public

BBa_K2066018
type: Component
persistent identity: component2523912
display id: component2523912
version: 1
top level: BBa_K2066055
definition: BBa_K2066018
access: public

BBa_K2066018
type: SequenceAnnotation
persistent identity: annotation2523912
display id: annotation2523912
version: 1
top level: BBa_K2066055
location: https://synbiohub.org/public/igem/BBa_K2066055/annotation2523912/range2523912/1
component: BBa_K2066018

https://synbiohub.org/public/igem/BBa_K2066055/annotation2523912/range2523912/1
type: Range
persistent identity: range2523912
display id: range2523912
version: 1
top level: BBa_K2066055
direction: direction/reverse
start: 1
end: 40
orientation: inline

BBa_K2066537
type: SequenceAnnotation
persistent identity: annotation2523913
display id: annotation2523913
version: 1
top level: BBa_K2066055
location: https://synbiohub.org/public/igem/BBa_K2066055/annotation2523913/range2523913/1
component: BBa_K2066537

https://synbiohub.org/public/igem/BBa_K2066055/annotation2523913/range2523913/1
type: Range
persistent identity: range2523913
display id: range2523913
version: 1
top level: BBa_K2066055
direction: direction/reverse
start: 41
end: 60
orientation: inline

BBa_K2066527
type: SequenceAnnotation
persistent identity: annotation2523914
display id: annotation2523914
version: 1
top level: BBa_K2066055
location: https://synbiohub.org/public/igem/BBa_K2066055/annotation2523914/range2523914/1
component: BBa_K2066527

https://synbiohub.org/public/igem/BBa_K2066055/annotation2523914/range2523914/1
type: Range
persistent identity: range2523914
display id: range2523914
version: 1
top level: BBa_K2066055
direction: direction/reverse
start: 61
end: 78
orientation: inline

BBa_K2066518
type: SequenceAnnotation
persistent identity: annotation2523915
display id: annotation2523915
version: 1
top level: BBa_K2066055
location: https://synbiohub.org/public/igem/BBa_K2066055/annotation2523915/range2523915/1
component: BBa_K2066518

https://synbiohub.org/public/igem/BBa_K2066055/annotation2523915/range2523915/1
type: Range
persistent identity: range2523915
display id: range2523915
version: 1
top level: BBa_K2066055
direction: direction/reverse
start: 79
end: 792
orientation: inline

BBa_K2066519
type: SequenceAnnotation
persistent identity: annotation2523916
display id: annotation2523916
version: 1
top level: BBa_K2066055
location: https://synbiohub.org/public/igem/BBa_K2066055/annotation2523916/range2523916/1
component: BBa_K2066519

https://synbiohub.org/public/igem/BBa_K2066055/annotation2523916/range2523916/1
type: Range
persistent identity: range2523916
display id: range2523916
version: 1
top level: BBa_K2066055
direction: direction/reverse
start: 793
end: 822
orientation: inline

BBa_K2066507
type: SequenceAnnotation
persistent identity: annotation2523917
display id: annotation2523917
version: 1
top level: BBa_K2066055
location: https://synbiohub.org/public/igem/BBa_K2066055/annotation2523917/range2523917/1
component: BBa_K2066507

https://synbiohub.org/public/igem/BBa_K2066055/annotation2523917/range2523917/1
type: Range
persistent identity: range2523917
display id: range2523917
version: 1
top level: BBa_K2066055
direction: direction/reverse
start: 823
end: 842
orientation: inline

BBa_K2066520
type: SequenceAnnotation
persistent identity: annotation2523918
display id: annotation2523918
version: 1
top level: BBa_K2066055
location: https://synbiohub.org/public/igem/BBa_K2066055/annotation2523918/range2523918/1
component: BBa_K2066520

https://synbiohub.org/public/igem/BBa_K2066055/annotation2523918/range2523918/1
type: Range
persistent identity: range2523918
display id: range2523918
version: 1
top level: BBa_K2066055
direction: direction/reverse
start: 843
end: 1076
orientation: inline

BBa_K2066021
type: SequenceAnnotation
persistent identity: annotation2523919
display id: annotation2523919
version: 1
top level: BBa_K2066055
location: https://synbiohub.org/public/igem/BBa_K2066055/annotation2523919/range2523919/1
component: BBa_K2066021

https://synbiohub.org/public/igem/BBa_K2066055/annotation2523919/range2523919/1
type: Range
persistent identity: range2523919
display id: range2523919
version: 1
top level: BBa_K2066055
direction: direction/reverse
start: 1077
end: 1116
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2523920
display id: annotation2523920
version: 1
top level: BBa_K2066055
location: https://synbiohub.org/public/igem/BBa_K2066055/annotation2523920/range2523920/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K2066055/annotation2523920/range2523920/1
type: Range
persistent identity: range2523920
display id: range2523920
version: 1
top level: BBa_K2066055
direction: direction/forward
start: 1117
end: 1196
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2523922
display id: annotation2523922
version: 1
top level: BBa_K2066055
location: https://synbiohub.org/public/igem/BBa_K2066055/annotation2523922/range2523922/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K2066055/annotation2523922/range2523922/1
type: Range
persistent identity: range2523922
display id: range2523922
version: 1
top level: BBa_K2066055
direction: direction/forward
start: 1197
end: 1237
orientation: inline

BBa_K2066019
type: SequenceAnnotation
persistent identity: annotation2523926
display id: annotation2523926
version: 1
top level: BBa_K2066055
location: https://synbiohub.org/public/igem/BBa_K2066055/annotation2523926/range2523926/1
component: BBa_K2066019

https://synbiohub.org/public/igem/BBa_K2066055/annotation2523926/range2523926/1
type: Range
persistent identity: range2523926
display id: range2523926
version: 1
top level: BBa_K2066055
direction: direction/reverse
start: 1238
end: 1277
orientation: inline

BBa_K2066018
type: ComponentDefinition
persistent identity: BBa K2066018
display id: BBa_K2066018
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 2 Sequence, from Torella et al., 2013
created: 2016-07-11T11:00:00Z
modified: 2016-10-19T05:41:43Z
mutable provenance: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
owned by: james
owned by: myers
mutable description: This is Unique Nucleotide Sequence 2, (UNS 2), from Torella et al., 2013. The William and Mary iGEM team has adopted this as our standard prefix; as such, all of our parts will have this sequence immediately following the BioBrick prefix. We took this measure in order to allow easier Gibson Assembly cloning of our parts. Primer sequences which can be used to clone with the UNS 2/3 standard can be found on our wiki.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: UNS 2 was chosen because it works well with UNS 3 and it is in accordance with the BioBrick standard.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K2066018_sequence/1

BBa_K2066021
type: ComponentDefinition
persistent identity: BBa K2066021
display id: BBa_K2066021
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 5 Sequence, from Torella et al., 2013
created: 2016-08-24T11:00:00Z
modified: 2016-10-19T05:45:29Z
mutable provenance: This part was synthesized as part of a gBlock / using IDT DNA synthesis. Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
owned by: james
owned by: myers
mutable description: This is Unique Nucleotide Sequence 5, (UNS 5), from Torella et al., 2013. The William and Mary iGEM team has adopted this as our standard prefix; as such, all of our parts will have this sequence immediately following the BioBrick prefix. We took this measure in order to allow easier Gibson Assembly cloning of our parts. Primer sequences which can be used to clone with the UNS 2/3 standard can be found on our wiki. The sequence for this part came from the following paper: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860. A huge thanks to all the researchers involved in its original creation!
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: UNS 5 was chosen along with UNS 4 to serve as primer binding sites which will create an amplicon that contains the base sequence for our ICA monomer but does not contain USN 2 or 3.
star: false
creator: Joseph L Maniaci
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066021_sequence/1

BBa_K2066527
type: ComponentDefinition
persistent identity: BBa K2066527
display id: BBa_K2066527
version: 1
was generated by: iGEM to SBOL conversion
description: B0034 with scar TACTAG
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T11:39:15Z
mutable provenance: Bba_B0034 + TACTAG
owned by: james
owned by: myers
mutable description: This is part B0034 with the scar region TACTAG added onto the part so that the correct scar region will be present in WM composite parts K2066059 - K20660108. This has no other purpose, but is just necessary do the system of the registry.
discontinued: false
dominant: false
group u list: _2534_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Joseph L Maniaci
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_K2066527_sequence/1

BBa_K2066507
type: ComponentDefinition
persistent identity: BBa K2066507
display id: BBa_K2066507
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 6.1 (Torella et. al 2013)
created: 2016-08-30T11:00:00Z
modified: 2016-10-19T06:10:34Z
mutable provenance: UNS 6.1 sequence derived from (Torella et. al 2013) "Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly".
owned by: james
owned by: myers
mutable description: This part is used as a spacer. WM iGEM 2016 used this part as a spacer to connect two separate composite parts onto the same plasmid. For example, UNS 6.1 was used as a spacer to connect K2066022(TetR on UNS) and K2066023(pTET GFP on UNS) to make K2066053.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 31541
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Used this part as a spacer.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066507_sequence/1

BBa_K2066518
type: ComponentDefinition
persistent identity: BBa K2066518
display id: BBa_K2066518
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry (with start codon)
created: 2016-10-07T11:00:00Z
modified: 2016-10-08T10:50:28Z
owned by: james
owned by: myers
mutable description: Modified from the mCherry used in K2066028 and K2066029 to include the start codon. This modified mCherry is used in the construction of K2066040 and K2066041.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 31544
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The start codon was removed in K2066028 and K2066029 to follow the design from Synthetic Analog Computation in Living Cells (Daniel et al.) where the codon bias was being optimized.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K2066518_sequence/1

BBa_K2066519
type: ComponentDefinition
persistent identity: BBa K2066519
display id: BBa_K2066519
version: 1
was generated by: iGEM to SBOL conversion
description: spacer sB
created: 2016-10-08T11:00:00Z
modified: 2016-10-08T11:01:16Z
mutable provenance: Pothoulakis et al. 2013 (???The Spinach RNA Aptamer as a Characterization Tool for Synthetic Biology???).
owned by: james
owned by: myers
mutable description: The spacer sB is taken from Pothoulakis et al. 2013 (???The Spinach RNA Aptamer as a Characterization Tool for Synthetic Biology???) and used in the construction of K2066040 and K2066041.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 31544
owning group id: 9
status: status/Planning
bookmark: false
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066519_sequence/1

BBa_K2066520
type: ComponentDefinition
persistent identity: BBa K2066520
display id: BBa_K2066520
version: 1
was generated by: iGEM to SBOL conversion
description: F30 scaffold / 2xBroccoli
created: 2016-10-08T11:00:00Z
modified: 2016-10-08T11:16:28Z
mutable provenance: The broccoli sequence is from https://www.addgene.org/66843/.
owned by: james
owned by: myers
mutable description: This part is meant for use in Promoter Characterization and RBS Characterization subproject for WM iGEM 2016.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 31544
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The part does have a SpeI cutsite in the Spinach aptamer. We???re going to use the original spinach to replicate the results of Pothoulakis et al. as exactly as possible and try to move to the Broccoli aptamer (which is RFC10 compatible) as soon as possible.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066520_sequence/1

BBa_K2066537
type: ComponentDefinition
persistent identity: BBa K2066537
display id: BBa_K2066537
version: 1
was generated by: iGEM to SBOL conversion
description: t7 promoter
created: 2016-10-13T11:00:00Z
modified: 2016-10-16T03:24:53Z
mutable provenance: ptm
owned by: james
owned by: myers
mutable description: tem
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 27446
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: p
star: false
creator: John Marken
type: DnaRegion
role: SO:0001207
role: partType/T7
sequence: https://synbiohub.org/public/igem/BBa_K2066537_sequence/1

BBa_K2066019
type: ComponentDefinition
persistent identity: BBa K2066019
display id: BBa_K2066019
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 3 Sequence, from Torella et al., 2013
created: 2016-07-11T11:00:00Z
modified: 2016-10-19T05:43:00Z
mutable provenance: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
owned by: james
owned by: myers
mutable description: This is Unique Nucleotide Sequence 3, (UNS 3), from Torella et al., 2013. The William and Mary iGEM team has adopted this as our standard prefix; as such, all of our parts will have this sequence immediately following the BioBrick prefix. We took this measure in order to allow easier Gibson Assembly cloning of our parts. Primer sequences which can be used to clone with the UNS 2/3 standard can be found on our wiki. The sequence for this part came from the following paper: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860. A huge thanks to all the researchers involved in its original creation!
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This UNS sequence was chosen to serve as the 3' primer in our standard because it works well with UNS 2 and it adheres to the BioBrick standards.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066019_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K2066055_sequence/1
type: Sequence
persistent identity: BBa K2066055 sequence
display id: BBa_K2066055_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgggagttcgtagacggaaacaaacgcagaatccaagctaatacgactcactatagggaaagaggagaaatactagatggtgagcaagggcgaagaagataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataactctacgacaacctcttcacagccaatctcctcgttcgctgccacctaagttgccatgtgtatgtgggagacggtcgggtccatctgagacggtcgggtccagatattcgtatctgtcgagtagagtgtgggctcagatgtcgagtagagtgtgggctcccacatactctgatgatccagacggtcgggtccatctgagacggtcgggtccagatattcgtatctgtcgagtagagtgtgggctcagatgtcgagtagagtgtgggctggatcattcatggcaagagccaactccctttacaacctcactcaagtccgttagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctcacactggctcaccttcgggtgggcctttctgcgtttatagcactgaaggtcctcaatcgcactggaaacatcaaggtcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066018_sequence/1
type: Sequence
persistent identity: BBa K2066018 sequence
display id: BBa_K2066018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgggagttcgtagacggaaacaaacgcagaatccaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066021_sequence/1
type: Sequence
persistent identity: BBa K2066021 sequence
display id: BBa_K2066021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagccaactccctttacaacctcactcaagtccgttagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066527_sequence/1
type: Sequence
persistent identity: BBa K2066527 sequence
display id: BBa_K2066527_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066507_sequence/1
type: Sequence
persistent identity: BBa K2066507 sequence
display id: BBa_K2066507_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgttcgctgccacctaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066518_sequence/1
type: Sequence
persistent identity: BBa K2066518 sequence
display id: BBa_K2066518_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaagaagataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066519_sequence/1
type: Sequence
persistent identity: BBa K2066519 sequence
display id: BBa_K2066519_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctctacgacaacctcttcacagccaatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066520_sequence/1
type: Sequence
persistent identity: BBa K2066520 sequence
display id: BBa_K2066520_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgccatgtgtatgtgggagacggtcgggtccatctgagacggtcgggtccagatattcgtatctgtcgagtagagtgtgggctcagatgtcgagtagagtgtgggctcccacatactctgatgatccagacggtcgggtccatctgagacggtcgggtccagatattcgtatctgtcgagtagagtgtgggctcagatgtcgagtagagtgtgggctggatcattcatggcaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066537_sequence/1
type: Sequence
persistent identity: BBa K2066537 sequence
display id: BBa_K2066537_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactataggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066019_sequence/1
type: Sequence
persistent identity: BBa K2066019 sequence
display id: BBa_K2066019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcactgaaggtcctcaatcgcactggaaacatcaaggtcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z