LOCUS       BBa_K2066078            1082 bp    DNA     linear       29-Nov-2024
DEFINITION  Promoter characterization part -  R0040 with RiboJ
ACCESSION   BBa_K2066078
VERSION     BBa_K2066078.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     misc_feature    41..59
                     /label=TetR 1
     misc_feature    41..94
                     /label=BBa_R0040
     promoter        60..65
                     /label=-35
     misc_feature    66..84
                     /label=TetR 2
     promoter        83..88
                     /label=-10
     promoter        41..94
                     /label=BBa_R0040
     misc_feature    95..175
                     /label=BBa_K2066506
     RBS             176..193
                     /label=BBa_K2066527
     misc_feature    194..913
                     /label=BBa_K2066509
     stem_loop       925..968
                     /label=stem_loop
     misc_feature    914..993
                     /label=BBa_B0010
     terminator      914..993
                     /label=BBa_B0010
     stem_loop       1009..1028
                     /label=T7 TE
     misc_feature    1035..1035
                     /label=stop
     polyA_site      1029..1042
                     /label=polya
     misc_feature    1002..1042
                     /label=BBa_B0012
     terminator      1002..1042
                     /label=BBa_B0012
     terminator      914..1042
                     /label=BBa_B0015
     misc_feature    1043..1082
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc tccctatcag tgatagagat
       61 tgacatccct atcagtgata gagatactga gcacagctgt caccggatgt gctttccggt
      121 ctgatgagtc cgtgaggacg aaacagcctc tacaaataat tttgtttaaa ctagaaaaga
      181 ggagaaatac tagatgcgta aaggcgaaga gctgttcact ggtgtcgtcc ctattctggt
      241 ggaactggat ggtgatgtca acggtcataa gttttccgtg cgtggcgagg gtgaaggtga
      301 cgcaactaat ggtaaactga cgctgaagtt catctgtact actggtaaac tgccggtacc
      361 ttggccgact ctggtaacga cgctgactta tggtgttcag tgctttgctc gttatccgga
      421 ccatatgaag cagcatgact tcttcaagtc cgccatgccg gaaggctatg tgcaggaacg
      481 cacgatttcc tttaaggatg acggcacgta caaaacgcgt gcggaagtga aatttgaagg
      541 cgataccctg gtaaaccgca ttgagctgaa aggcattgac tttaaagaag acggcaatat
      601 cctgggccat aagctggaat acaattttaa cagccacaat gtgtacatta ccgcagataa
      661 acaaaaaaat ggcattaaag cgaatttcaa aattcgccac aacgtggagg atggcagcgt
      721 gcagctggct gatcactacc agcaaaacac tccaatcggt gatggtcctg ttctgctgcc
      781 agacaatcac tatctgagca cgcaaagcgt tctgtctaaa gatccgaacg agaaacgcga
      841 tcatatggtt ctgctggagt tcgtaaccgc agcgggcatc acgcatggta tggatgaact
      901 gtacaaatga tgaccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      961 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1021 ggtgggcctt tctgcgttta tagcactgaa ggtcctcaat cgcactggaa acatcaaggt
     1081 cg
//