LOCUS       BBa_K2066079            1228 bp    DNA     linear       07-Dec-2025
DEFINITION  Promoter characterization part - R0010 with RiboJ
ACCESSION   BBa_K2066079
VERSION     BBa_K2066079.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     CDS             41..128
                     /label=end of LacI coding region (inactive)
     misc_feature    41..240
                     /label=BBa_R0010
     misc_feature    129..166
                     /label=CAP binding site
     promoter        177..182
                     /label=-35
     promoter        201..206
                     /label=-10
     misc_feature    206..240
                     /label=LacI binding site
     misc_feature    213..213
                     /label=start
     promoter        41..240
                     /label=BBa_R0010
     misc_feature    241..321
                     /label=BBa_K2066506
     RBS             322..339
                     /label=BBa_K2066527
     misc_feature    340..1059
                     /label=BBa_K2066509
     stem_loop       1071..1114
                     /label=stem_loop
     misc_feature    1060..1139
                     /label=BBa_B0010
     terminator      1060..1139
                     /label=BBa_B0010
     stem_loop       1155..1174
                     /label=T7 TE
     misc_feature    1181..1181
                     /label=stop
     polyA_site      1175..1188
                     /label=polya
     misc_feature    1148..1188
                     /label=BBa_B0012
     terminator      1148..1188
                     /label=BBa_B0012
     terminator      1060..1188
                     /label=BBa_B0015
     misc_feature    1189..1228
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc caatacgcaa accgcctctc
       61 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
      121 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
      181 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
      241 agctgtcacc ggatgtgctt tccggtctga tgagtccgtg aggacgaaac agcctctaca
      301 aataattttg tttaaactag aaaagaggag aaatactaga tgcgtaaagg cgaagagctg
      361 ttcactggtg tcgtccctat tctggtggaa ctggatggtg atgtcaacgg tcataagttt
      421 tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct gaagttcatc
      481 tgtactactg gtaaactgcc ggtaccttgg ccgactctgg taacgacgct gacttatggt
      541 gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt caagtccgcc
      601 atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg cacgtacaaa
      661 acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga gctgaaaggc
      721 attgacttta aagaagacgg caatatcctg ggccataagc tggaatacaa ttttaacagc
      781 cacaatgtgt acattaccgc agataaacaa aaaaatggca ttaaagcgaa tttcaaaatt
      841 cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca aaacactcca
      901 atcggtgatg gtcctgttct gctgccagac aatcactatc tgagcacgca aagcgttctg
      961 tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt aaccgcagcg
     1021 ggcatcacgc atggtatgga tgaactgtac aaatgatgac caggcatcaa ataaaacgaa
     1081 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1141 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatagc actgaaggtc
     1201 ctcaatcgca ctggaaacat caaggtcg
//