LOCUS       BBa_K2066099            1001 bp    DNA     linear       15-Jun-2024
DEFINITION  Promoter characterization part - R0040 without RiboJ
ACCESSION   BBa_K2066099
VERSION     BBa_K2066099.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     misc_feature    41..59
                     /label=TetR 1
     misc_feature    41..94
                     /label=BBa_R0040
     promoter        60..65
                     /label=-35
     misc_feature    66..84
                     /label=TetR 2
     promoter        83..88
                     /label=-10
     promoter        41..94
                     /label=BBa_R0040
     RBS             95..112
                     /label=BBa_K2066527
     misc_feature    113..832
                     /label=BBa_K2066509
     stem_loop       844..887
                     /label=stem_loop
     misc_feature    833..912
                     /label=BBa_B0010
     terminator      833..912
                     /label=BBa_B0010
     stem_loop       928..947
                     /label=T7 TE
     misc_feature    954..954
                     /label=stop
     polyA_site      948..961
                     /label=polya
     misc_feature    921..961
                     /label=BBa_B0012
     terminator      921..961
                     /label=BBa_B0012
     terminator      833..961
                     /label=BBa_B0015
     misc_feature    962..1001
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc tccctatcag tgatagagat
       61 tgacatccct atcagtgata gagatactga gcacaaagag gagaaatact agatgcgtaa
      121 aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa
      181 cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac
      241 gctgaagttc atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac
      301 gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt
      361 cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga
      421 cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat
      481 tgagctgaaa ggcattgact ttaaagaaga cggcaatatc ctgggccata agctggaata
      541 caattttaac agccacaatg tgtacattac cgcagataaa caaaaaaatg gcattaaagc
      601 gaatttcaaa attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca
      661 gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac
      721 gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt
      781 cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaatgat gaccaggcat
      841 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      901 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
      961 agcactgaag gtcctcaatc gcactggaaa catcaaggtc g
//