BBa_K2066100
type: ComponentDefinition
persistent identity: BBa K2066100
display id: BBa_K2066100
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter characterization part - R0011 with RiboJ
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T08:09:49Z
mutable provenance: This part was created using Gibson Assembly methods. We used K2066035 as a template and an amplicon containing the relevant promoter sequence +/- RiboJ combination as the insert. RiboJ sequence sfGFP sequence from Lou et al., Supplement section V. sfGFP sequence derived and modified from Lou et al., Supplement section V. The UNS sequences are from Torella et al. 2013.
owned by: james
owned by: myers
mutable description: The part is flanked by UNS 2 and UNS 3 as per the WM UNS gibson cloning standard. This part is used to characterize the promoter part Bba_R0011 with riboJ insulation so that the effects of RiboJ insulation on gene expression can be characterized using the corresponding WM part K2066102. This part on 1C3 can be cotransformed into cells along with K2066016 (preferably on pSB3K3 or other low copy backbone for ideal induction behavior) and the resulting IPTG-induction curve can be compared to those of other parts (K2066059 - K20660108) with different promoter / RiboJ variations.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 31541
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This part was designed to perform RiboJ characterization verifying and building on that performed in Lou et al. 2012 (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???). This part will be used in concert with WM16_015 (plLACO-1 Ribozyme Chracterization Part ??? cI GFP) to determine the influence of the RiboJ insulator sequence on gene expression.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K2066018
component: BBa_K2066506
component: BBa_R0011
component: BBa_K2066509
component: BBa_K2066527
component: BBa_K2066019
component: BBa_B0015
sequence annotation: BBa_K2066018
sequence annotation: BBa_R0011
sequence annotation: BBa_K2066506
sequence annotation: BBa_K2066527
sequence annotation: BBa_K2066509
sequence annotation: BBa_B0015
sequence annotation: BBa_K2066019
sequence: https://synbiohub.org/public/igem/BBa_K2066100_sequence/1

BBa_K2066018
type: Component
persistent identity: component2510510
display id: component2510510
version: 1
top level: BBa_K2066100
definition: BBa_K2066018
access: public

BBa_K2066506
type: Component
persistent identity: component2510516
display id: component2510516
version: 1
top level: BBa_K2066100
definition: BBa_K2066506
access: public

BBa_R0011
type: Component
persistent identity: component2510511
display id: component2510511
version: 1
top level: BBa_K2066100
definition: lacI+pL
access: public

BBa_K2066509
type: Component
persistent identity: component2510518
display id: component2510518
version: 1
top level: BBa_K2066100
definition: BBa_K2066509
access: public

BBa_K2066527
type: Component
persistent identity: component2510517
display id: component2510517
version: 1
top level: BBa_K2066100
definition: BBa_K2066527
access: public

BBa_K2066019
type: Component
persistent identity: component2510526
display id: component2510526
version: 1
top level: BBa_K2066100
definition: BBa_K2066019
access: public

BBa_B0015
type: Component
persistent identity: component2510525
display id: component2510525
version: 1
top level: BBa_K2066100
definition: BBa_B0015
access: public

BBa_K2066018
type: SequenceAnnotation
persistent identity: annotation2510510
display id: annotation2510510
version: 1
top level: BBa_K2066100
location: https://synbiohub.org/public/igem/BBa_K2066100/annotation2510510/range2510510/1
component: BBa_K2066018

https://synbiohub.org/public/igem/BBa_K2066100/annotation2510510/range2510510/1
type: Range
persistent identity: range2510510
display id: range2510510
version: 1
top level: BBa_K2066100
direction: direction/reverse
start: 1
end: 40
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2510511
display id: annotation2510511
version: 1
top level: BBa_K2066100
location: https://synbiohub.org/public/igem/BBa_K2066100/annotation2510511/range2510511/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K2066100/annotation2510511/range2510511/1
type: Range
persistent identity: range2510511
display id: range2510511
version: 1
top level: BBa_K2066100
direction: direction/forward
start: 41
end: 94
orientation: inline

BBa_K2066506
type: SequenceAnnotation
persistent identity: annotation2510516
display id: annotation2510516
version: 1
top level: BBa_K2066100
location: https://synbiohub.org/public/igem/BBa_K2066100/annotation2510516/range2510516/1
component: BBa_K2066506

https://synbiohub.org/public/igem/BBa_K2066100/annotation2510516/range2510516/1
type: Range
persistent identity: range2510516
display id: range2510516
version: 1
top level: BBa_K2066100
direction: direction/reverse
start: 96
end: 176
orientation: inline

BBa_K2066527
type: SequenceAnnotation
persistent identity: annotation2510517
display id: annotation2510517
version: 1
top level: BBa_K2066100
location: https://synbiohub.org/public/igem/BBa_K2066100/annotation2510517/range2510517/1
component: BBa_K2066527

https://synbiohub.org/public/igem/BBa_K2066100/annotation2510517/range2510517/1
type: Range
persistent identity: range2510517
display id: range2510517
version: 1
top level: BBa_K2066100
direction: direction/reverse
start: 177
end: 194
orientation: inline

BBa_K2066509
type: SequenceAnnotation
persistent identity: annotation2510518
display id: annotation2510518
version: 1
top level: BBa_K2066100
location: https://synbiohub.org/public/igem/BBa_K2066100/annotation2510518/range2510518/1
component: BBa_K2066509

https://synbiohub.org/public/igem/BBa_K2066100/annotation2510518/range2510518/1
type: Range
persistent identity: range2510518
display id: range2510518
version: 1
top level: BBa_K2066100
direction: direction/reverse
start: 195
end: 914
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2510525
display id: annotation2510525
version: 1
top level: BBa_K2066100
location: https://synbiohub.org/public/igem/BBa_K2066100/annotation2510525/range2510525/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2066100/annotation2510525/range2510525/1
type: Range
persistent identity: range2510525
display id: range2510525
version: 1
top level: BBa_K2066100
direction: direction/reverse
start: 915
end: 1043
orientation: inline

BBa_K2066019
type: SequenceAnnotation
persistent identity: annotation2510526
display id: annotation2510526
version: 1
top level: BBa_K2066100
location: https://synbiohub.org/public/igem/BBa_K2066100/annotation2510526/range2510526/1
component: BBa_K2066019

https://synbiohub.org/public/igem/BBa_K2066100/annotation2510526/range2510526/1
type: Range
persistent identity: range2510526
display id: range2510526
version: 1
top level: BBa_K2066100
direction: direction/reverse
start: 1044
end: 1083
orientation: inline

BBa_K2066018
type: ComponentDefinition
persistent identity: BBa K2066018
display id: BBa_K2066018
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 2 Sequence, from Torella et al., 2013
created: 2016-07-11T11:00:00Z
modified: 2016-10-19T05:41:43Z
mutable provenance: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
owned by: james
owned by: myers
mutable description: This is Unique Nucleotide Sequence 2, (UNS 2), from Torella et al., 2013. The William and Mary iGEM team has adopted this as our standard prefix; as such, all of our parts will have this sequence immediately following the BioBrick prefix. We took this measure in order to allow easier Gibson Assembly cloning of our parts. Primer sequences which can be used to clone with the UNS 2/3 standard can be found on our wiki.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: UNS 2 was chosen because it works well with UNS 3 and it is in accordance with the BioBrick standard.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K2066018_sequence/1

BBa_K2066019
type: ComponentDefinition
persistent identity: BBa K2066019
display id: BBa_K2066019
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 3 Sequence, from Torella et al., 2013
created: 2016-07-11T11:00:00Z
modified: 2016-10-19T05:43:00Z
mutable provenance: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
owned by: james
owned by: myers
mutable description: This is Unique Nucleotide Sequence 3, (UNS 3), from Torella et al., 2013. The William and Mary iGEM team has adopted this as our standard prefix; as such, all of our parts will have this sequence immediately following the BioBrick prefix. We took this measure in order to allow easier Gibson Assembly cloning of our parts. Primer sequences which can be used to clone with the UNS 2/3 standard can be found on our wiki. The sequence for this part came from the following paper: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860. A huge thanks to all the researchers involved in its original creation!
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This UNS sequence was chosen to serve as the 3' primer in our standard because it works well with UNS 2 and it adheres to the BioBrick standards.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066019_sequence/1

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K2066527
type: ComponentDefinition
persistent identity: BBa K2066527
display id: BBa_K2066527
version: 1
was generated by: iGEM to SBOL conversion
description: B0034 with scar TACTAG
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T11:39:15Z
mutable provenance: Bba_B0034 + TACTAG
owned by: james
owned by: myers
mutable description: This is part B0034 with the scar region TACTAG added onto the part so that the correct scar region will be present in WM composite parts K2066059 - K20660108. This has no other purpose, but is just necessary do the system of the registry.
discontinued: false
dominant: false
group u list: _2534_
m user id: 27446
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Joseph L Maniaci
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K2066527_sequence/1

BBa_K2066506
type: ComponentDefinition
persistent identity: BBa K2066506
display id: BBa_K2066506
version: 1
was generated by: iGEM to SBOL conversion
description: RiboJ (Ribozyme Insulator) Lou et. al. 2012
created: 2016-08-30T11:00:00Z
modified: 2016-10-12T12:27:33Z
mutable provenance: Part sequence is from Lou et al. 2012, Supplemental Section V (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???).
owned by: james
owned by: myers
mutable description: RiboJ is the sequence for a ribozyme studied in Lou et. al 2012 ("Ribozyme-based insulator parts buffer synthetic circuits from genetic context"). WM iGEM 2016 used this sequence between the promoter and ribosome sequence. One of our goals for using this part is moving it onto a Biobrick backbone. Furthermore, In Lou et. al, this ribozyme sequence was said to act as an insulator which generalizes protein expression levels for a given promoter. We used RiboJ to collect data for our Ribozyme characterization project as well as our ribosome and promoter characterization projects.
discontinued: false
dominant: false
group u list: _2534_
m user id: 27446
owner id: 31541
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We designed this part to use as an insulator and also move this riboJ sequence onto a Biobrick backbone.
star: false
creator: Likhitha Kolla
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K2066506_sequence/1

BBa_K2066509
type: ComponentDefinition
persistent identity: BBa K2066509
display id: BBa_K2066509
version: 1
was generated by: iGEM to SBOL conversion
description: sfGFP
created: 2016-08-30T11:00:00Z
modified: 2016-10-19T02:54:25Z
mutable provenance: The sequence for this sfGFP reporter gene is modified from Lou et al. Supplement section V. This is the sequence of superfolder GFP BBa_I746916, but with four codon modifications to match WM16_015: at position 441, G->T. At 446, C->T. At 495, T->C. At 562, C->A.
owned by: james
owned by: myers
mutable description: The part is a reporter used for K2066014.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 31541
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Design inspired by Lou et. al. 2012
star: false
creator: Likhitha Kolla
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence: https://synbiohub.org/public/igem/BBa_K2066509_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K2066100_sequence/1
type: Sequence
persistent identity: BBa K2066100 sequence
display id: BBa_K2066100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgggagttcgtagacggaaacaaacgcagaatccaagcaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacaagctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaaactagaaaagaggagaaatactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtgtacattaccgcagataaacaaaaaaatggcattaaagcgaatttcaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagcactgaaggtcctcaatcgcactggaaacatcaaggtcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066018_sequence/1
type: Sequence
persistent identity: BBa K2066018 sequence
display id: BBa_K2066018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgggagttcgtagacggaaacaaacgcagaatccaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066019_sequence/1
type: Sequence
persistent identity: BBa K2066019 sequence
display id: BBa_K2066019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcactgaaggtcctcaatcgcactggaaacatcaaggtcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066527_sequence/1
type: Sequence
persistent identity: BBa K2066527 sequence
display id: BBa_K2066527_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066506_sequence/1
type: Sequence
persistent identity: BBa K2066506 sequence
display id: BBa_K2066506_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaaactaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066509_sequence/1
type: Sequence
persistent identity: BBa K2066509 sequence
display id: BBa_K2066509_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtgtacattaccgcagataaacaaaaaaatggcattaaagcgaatttcaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z