LOCUS       BBa_K2066101            1147 bp    DNA     linear       15-Jun-2024
DEFINITION  Promoter characterization part - R0010 without RiboJ
ACCESSION   BBa_K2066101
VERSION     BBa_K2066101.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     CDS             41..128
                     /label=end of LacI coding region (inactive)
     misc_feature    41..240
                     /label=BBa_R0010
     misc_feature    129..166
                     /label=CAP binding site
     promoter        177..182
                     /label=-35
     promoter        201..206
                     /label=-10
     misc_feature    206..240
                     /label=LacI binding site
     misc_feature    213..213
                     /label=start
     promoter        41..240
                     /label=BBa_R0010
     RBS             241..258
                     /label=BBa_K2066527
     misc_feature    259..978
                     /label=BBa_K2066509
     stem_loop       990..1033
                     /label=stem_loop
     misc_feature    979..1058
                     /label=BBa_B0010
     terminator      979..1058
                     /label=BBa_B0010
     stem_loop       1074..1093
                     /label=T7 TE
     misc_feature    1100..1100
                     /label=stop
     polyA_site      1094..1107
                     /label=polya
     misc_feature    1067..1107
                     /label=BBa_B0012
     terminator      1067..1107
                     /label=BBa_B0012
     terminator      979..1107
                     /label=BBa_B0015
     misc_feature    1108..1147
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc caatacgcaa accgcctctc
       61 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
      121 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
      181 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
      241 aaagaggaga aatactagat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt
      301 ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa
      361 ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg
      421 gtaccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat
      481 ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag
      541 gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt
      601 gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaagacggc
      661 aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgtgta cattaccgca
      721 gataaacaaa aaaatggcat taaagcgaat ttcaaaattc gccacaacgt ggaggatggc
      781 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg
      841 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa
      901 cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat
      961 gaactgtaca aatgatgacc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1021 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1081 cttcgggtgg gcctttctgc gtttatagca ctgaaggtcc tcaatcgcac tggaaacatc
     1141 aaggtcg
//