LOCUS       BBa_K2066102            1002 bp    DNA     linear       25-Nov-2024
DEFINITION  Promoter characterization part - R0011 without RiboJ
ACCESSION   BBa_K2066102
VERSION     BBa_K2066102.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     misc_feature    43..59
                     /label=lac O1
     promoter        60..65
                     /label=-35
     misc_feature    66..82
                     /label=lac O1
     promoter        83..88
                     /label=-10
     misc_feature    41..94
                     /label=BBa_R0011
     promoter        41..94
                     /label=BBa_R0011
     RBS             96..113
                     /label=BBa_K2066527
     misc_feature    114..833
                     /label=BBa_K2066509
     stem_loop       845..888
                     /label=stem_loop
     misc_feature    834..913
                     /label=BBa_B0010
     terminator      834..913
                     /label=BBa_B0010
     stem_loop       929..948
                     /label=T7 TE
     misc_feature    955..955
                     /label=stop
     polyA_site      949..962
                     /label=polya
     misc_feature    922..962
                     /label=BBa_B0012
     terminator      922..962
                     /label=BBa_B0012
     terminator      834..962
                     /label=BBa_B0015
     misc_feature    963..1002
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc aattgtgagc ggataacaat
       61 tgacattgtg agcggataac aagatactga gcacaaaaga ggagaaatac tagatgcgta
      121 aaggcgaaga gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca
      181 acggtcataa gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga
      241 cgctgaagtt catctgtact actggtaaac tgccggtacc ttggccgact ctggtaacga
      301 cgctgactta tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact
      361 tcttcaagtc cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg
      421 acggcacgta caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca
      481 ttgagctgaa aggcattgac tttaaagaag acggcaatat cctgggccat aagctggaat
      541 acaattttaa cagccacaat gtgtacatta ccgcagataa acaaaaaaat ggcattaaag
      601 cgaatttcaa aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc
      661 agcaaaacac tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagca
      721 cgcaaagcgt tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt
      781 tcgtaaccgc agcgggcatc acgcatggta tggatgaact gtacaaatga tgaccaggca
      841 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      901 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      961 tagcactgaa ggtcctcaat cgcactggaa acatcaaggt cg
//