LOCUS       BBa_K2066109            1922 bp    DNA     linear       16-Feb-2025
DEFINITION  pTet GFP + TetR (weakened)
ACCESSION   BBa_K2066109
VERSION     BBa_K2066109.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     misc_feature    41..59
                     /label=TetR 1
     misc_feature    41..94
                     /label=BBa_R0040
     promoter        60..65
                     /label=-35
     misc_feature    66..84
                     /label=TetR 2
     promoter        83..88
                     /label=-10
     promoter        41..94
                     /label=BBa_R0040
     misc_feature    95..102
                     /label=BBa_K2066529
     misc_feature    107..110
                     /label=conserved
     RBS             103..114
                     /label=BBa_B0034
     misc_feature    115..120
                     /label=BBa_K2066530
     CDS             121..840
                     /label=GFP protein
     CDS             121..840
                     /label=BBa_E0040
     misc_feature    841..848
                     /label=BBa_K2066529
     stem_loop       860..903
                     /label=stem_loop
     misc_feature    849..928
                     /label=BBa_B0010
     terminator      849..928
                     /label=BBa_B0010
     stem_loop       944..963
                     /label=T7 TE
     misc_feature    970..970
                     /label=stop
     polyA_site      964..977
                     /label=polya
     misc_feature    937..977
                     /label=BBa_B0012
     terminator      937..977
                     /label=BBa_B0012
     terminator      849..977
                     /label=BBa_B0015
     misc_feature    978..997
                     /label=BBa_K2066507
     promoter        998..1032
                     /label=BBa_J23105
     misc_feature    1033..1040
                     /label=BBa_K2066529
     misc_feature    1047..1050
                     /label=conserved
     RBS             1041..1054
                     /label=BBa_B0031
     misc_feature    1055..1060
                     /label=BBa_K2066530
     CDS             1064..1680
                     /label=tetR
     misc_feature    1681..1714
                     /label=SsrA
     misc_feature    1721..1745
                     /label=Help:Barcodes
     CDS             1061..1745
                     /label=BBa_C0040
     misc_feature    1746..1753
                     /label=BBa_K2066529
     stem_loop       1765..1808
                     /label=stem_loop
     misc_feature    1754..1833
                     /label=BBa_B0010
     terminator      1754..1833
                     /label=BBa_B0010
     stem_loop       1849..1868
                     /label=T7 TE
     misc_feature    1875..1875
                     /label=stop
     polyA_site      1869..1882
                     /label=polya
     misc_feature    1842..1882
                     /label=BBa_B0012
     terminator      1842..1882
                     /label=BBa_B0012
     terminator      1754..1882
                     /label=BBa_B0015
     misc_feature    1883..1922
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc tccctatcag tgatagagat
       61 tgacatccct atcagtgata gagatactga gcactactag agaaagagga gaaatactag
      121 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      181 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      241 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      301 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      361 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      421 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      481 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      541 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      601 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      661 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      721 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      781 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
      841 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      901 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      961 gcctttctgc gtttatactc gttcgctgcc acctaagttt acggctagct cagtcctagg
     1021 tactatgcta gctactagag tcacacagga aacctactag atgtccagat tagataaaag
     1081 taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac
     1141 ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa
     1201 taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt
     1261 ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag
     1321 atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga
     1381 aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact
     1441 agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga
     1501 agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc
     1561 gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt
     1621 attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc
     1681 cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag
     1741 atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1801 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1861 ggtgggcctt tctgcgttta tagcactgaa ggtcctcaat cgcactggaa acatcaaggt
     1921 cg
//